Video

Nice

Hi pc pc p0

But how can you sterilize the parafilm?

it is helpfull

Very clear video

My teacher used your video as an instruction video, be proud

Why you adjust the pH to 8.8?

How do you clean the fritted glass after use?

This process needs practice more and more😮

You can add a little amount of resolving gel with higher percentage of TEMED to prevent leaky and after the thin layer of the bottom becom solid , you can add standard resolving gel

Can anyone here educate me how to prepare prf( protein rich fibrin) to use in infected wounds as I m working at periphery n not much facilities r available here , we try to heal n help patients on our own within our resources... I ve got a centrifuge old machine China made 800 centrifuge wth rpm:4000.n rcf:1790×g.capacity 20cc×5. I also wanna know test tube's length its material n time to spin to retrieve max benefit from my produce.though I have general info frm watching diff u tube video but wanna get know frm experts as reading comments here I noticed that quite an experts in centrifuge technique are commenting here. I wd really appreciate if anyone is to extend his knowledge to where it's really needed n be used productively.. Thanks in anticipation n prayers!

Thanks. Just what I was looking for. Don’t hold the Petri dish too tightly, they crack easily.

If the filter base stuck, how to remove it?

I love how early 2000's this video feels

Thanks for the nice video. Lately sometime I fail to make perfect well, and I realize it when I load my sample it won’t go through coz there are this sheet of the gel between the well. Might anyone have a suggestion? Many thanks

I do not see a sterile process ! The tape is already contaminated with ambient contaminants, So how to apply perfectly sterile ? Perfectly sterile. because the tape is not sterile as we can see. I need an idea!

You have a very good video. 2 things that would set you apart from others...1. list the glassware and tell where we can purchase. 2. Answer peoples questions. Those who ignore simple questions are only in it for themselves and I will not give any likes or watch any more of a persons work if they are so selfish.

Thanks mate ❤️

thanks 🙏🏻

Thank you for stopping my tears 😩

I had tried to filter the buffer through the same glass assembly, but I was facing a leakage problem from the filter base and funnel. I had tried so much to fix it but couldn't do so. Is there any technique to assemble the funnel and base with a clamp? Can you please help me out?

Thanks

Hi cations and anions, While loading my samples, the samples did not enter into the well but floated... Can you help me troubleshoot this? Thanks

Hi, I just came across this channel and The Lab channel. You guys are doing a fantastic and amazing job. However, what happened? Where have you been? It's been 10 years.

Can you please explain how to tell which side up for a 20micron membrane filter, thanks 🙏

PLEASE MAKE VIDEOS ON THE PROCESSES OF RECOMBINANT DNA TECHNOLOGY ?

WHAT IS REPORTER GENE ?

HOW DO WE KNOW CELL HAS BEEN TRANSFORMED ?

WHAT IS THE QUALIFICATION REQUIRED FOR THIS JOB , WHAT ARE THE PROSPECTS OF THIS AND WHAT ARE THE PRODUCTS THAT CAN BE MADE ?

Have u got any protocol for prepration of the reagents.

Thank you!

Thank you brother... helpful for me

Thank you so much. I wasted so much parafilm, i thought you would need a big piece xD

Legends are here from 2022__😂😂

How much is centrifuge

I saw my gel getting shrunk after staining & destaining (CBB). Staining- 10 mins- generally I stain it for 10 mins & destaining-30 mins.

Hi. I hv 2 questions. Why add isopropanol to removes bubbles and why is there stacking and resolving gel ?

Hello, I hope you're still active on this channel... Would there be an issue if you use 10X running buffer instead of 1X? Also, I noticed you had a very thin pipette tip that seemed to allow you to insert the sample past the short window and access very close to the bottom of the well. I was using a regular tip and found I couldnt get past the short window so I had to gently release the sample from the point of the short window. It appeared that the dyed sample 'fell' down into the well ok but I'm wondering if the sample may have dispersed more than I could see and could have contaminated other wells? Thanks for the video!

What does the gel look like in the end??

01:09

Thank You

Thank you for such informative video

thanks for the video...it's really useful for me

WAOUH

But I only have one pH meter that needs to be calibrated...

It's chemical transformation

Conk

can you able to provide detail protocol how i can run a page gel and try to do stain. please help me to learn

How much vaccume should be ?

Hi, exellent video. I have a question, how to tag a specific protein with antibodies?