MAKE UP TO $5000 USING THE BIOCOUPLER™!
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- čas přidán 21. 09. 2022
- Want to learn how you can make up to $2,500 using just a single BioCoupler? - Our Lab Director Francisco shows you how in this video! Get 10%OFF the tissue culture products you need using my code: www.plantcelltechnology.com/?...
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We are Plant Cell Technology, a leading plant tissue culture industry organization since 1995. We develop, produce, and market innovative solutions for global plant tissue culture operations and hobbyists. Our well-researched, certified, and trusted products keep your cultures healthy and contaminate-free.
#PlantCellTechnology #TissueCulture #Propagation #MicroPropagation #Plants #Labratory #Lab #TCLab #LiquidMediaCultures #MicroBiology #Cultures #LiquidMedia #TC #planttest #tissueculturetest - Věda a technologie
i purchased bios and all the stuff it is so much fun and relaxing, you guys are great
ooo.. That's so amazing! Great to know about it. Hope your experiments do great!
I've watched every episode of Francisco's videos and i've finally caught up. i'm inspired to come back to this profession after 30 years.
My question for the biocoupler is,
do you use the same liquid media for the whole of the life of the culture, or do you have to replenish after a few months?
Thankyou in advance.
Thank you so much for your kind words. We have forwarded this question to Francisco. so, will provide you with the answer as soon as he responds. Thanks!
He has a video that goes over it where he upgrades and moves plants to larger bio couplers with new media, it's just like tissue culture without the gelling agent, he also said exactly that in this video. Also, based on his videos, he has left things in tissue cultures for as much as a year or in an extreme case, two. So every few months, no. If the media remains sterile, and there is enough to coat the plant when you invert it, you're fine.
Hope you get better quickly. I have been missing your videos. Do you have a protocol for any Hostas? I have been doing the traditional propagation method but would like to move to TC for my Hosta plants. I am still new to all this. I have your starter kit but have not tried it.
Currently, we aren't working on Hostas plants in our lab. However, here's an article that you may find useful: sites.psu.edu/horticulture202/2015/10/18/herbaceous-plant-tissue-culture-hosta/
Very interesting. I would like to see the bio coupler used in a protocol
Sure!
I have built about 200 bio-couplers in the same style as your original version, but I couldn't find the filter material, but I have been working on a system for being able to add time released oxygen, it involves porphyrins and fine copper mesh, still not sure how it will go... I want to make something like a bio ball(filter media) that converts co2 to oxygen, that would be so cool. I feel like the bigger issue is finding a way to increase c02 in the system to feed the plants though, I am not sure what would be a better thing to try and achieve, since I assume the plants are creating oxygen from the CO2 at least half of the time. Hrmmmm...
I love these videos
Thanks, @gabrielbronsztein9467! Glad you're enjoying the videos!
hey, ive always wondered why don't you put plants in both sides of the bio coupler, or 2 container TIB systems in general
The concept of a temporary immersion bioreactor is that the plants are immersed in media solution for only a temporary period. It avoids problems like vitrification and gives your cultured plant tissues more room to grow without hindrance by absorbing just enough nutrients. So, for this, you need this kind of system.
After doing the multiplication media in the biocoupler, do you then do rooting media in the biocoupler and then after that, transplant to the fluval stratum? Or do you go from the multiplication media directly into the fluval stratum? Thanks!
We move for rooting and then acclimation. However, for some species, rooting is performed in the fluval stratum. So, it's based on the type of plant you are working with.
Like, some plants root readily in Fluval Stratum, while others benefit from a dedicated rooting medium.
So, research the specific needs of your plant.
Could you please do a video on embryo extraction from a dormant seed and moving it to tissue culture. Thanks
Sure! We can try in near future!
@@PlantCellTechnology Excellent, thank you
So there is no separate rooting stage?
The same media is used from start to finish? I definitely want to order some and try this out but I'm not sure what mix to start with since it looks like an all-in-one. Anthurium are my primary plants
I absolutely love your channel. I will place some supply orders from you for the content you provide
Hi,
No, the same media is not used for all stages. Tissue culture has four stages: establishment, multiplication, rooting, and acclimation.
You use the Biocoupler for the multiplication of plants.
To get started in anthurium, check out some of our videos on our channel:www.youtube.com/@PlantCellTechnology/search?query=anthurium
Also, here's the link to the blogs we've created on the plant: www.plantcelltechnology.com/search.php?search_query=anthurium§ion=content
Our starter kit will be great for you to get started in tissue culture. And, then as you proceed to the multiplication and rooting stage you will need plant hormones that also you can buy from our store.
Hope the info help!
Let us know if have any further questions.
Happy culturing!
You can do a rooting stage if you wanted to, if you don't feel there are enough roots, but in a biocoupler that can be a mess, might wanna move to a humidity dome for root development and acclimation, the two can and should happen at the same time under the correct conditions. Major tissue culture producers move plants to new cups with a much lower dose of multiplication hormones, or none at all, and an appropriate amount of rooting hormones during the last stage before sale. Some will also add hormones that make plants grow larger at the same time. Multiplication is just multiplication, plants can only do so much at one time.
Hey! Like your videos.
Quick question: Does basic-MS Medium already contain sucrose? I read it contains 30g per liter but I have seen reports where people said they supplemented it with 3% w/v sucrose for Philodendron...
No. PCT MS media is not supplemented with sucrose.
@@PlantCellTechnology So do you mean there is no sugar in there or I dont have to supplement it? Thanks
@@adrikong8923 I am saying that the commercially available PCT MS media do not contain sugar. But, as you definitely need sugar in the tissue culture media, so you will have to add it from the outside.
Just found your channel . I am looking for a consultant to get a lab started in Jamaica. It would nice if we link up . Would purchase those philodendron.
Hi. We do have consultation services that enable you to talk to Francisco about the lab setting or the tissue culture process. This will be immensely useful for you in this case when you want to build a lab. If interested, book a call here: www.plantcelltechnology.com/regular-plant-consultation/
Will the Biocoupler Bundle Work for Mini Sinningia/ Gesneriads? Or should I find a different liquid media mix to use with PPM?
For different plants, the type and concentration of hormones vary. So, you should definitely try to customize it.
The biocoupler works wonders for the multiplication and rooting stages, so it would be best to use it.
Were can I buy this biocuplar in Johannesburg South Africa
You can order from our website or contact our local distributor. You can find their details on this page: www.plantcelltechnology.com/distributors/
Could you use a biocoupler to multiply online bought plants that I receive in TC? Just take it out and put in multiplication media in the biocoupler?
Yes, you can subculture plants directly to the BioCouplers
Can I use biocoupler for potato tissue culture
Of course, you can! You can use Biocoupler to multiply any plant you want in tissue culture.
what is the that soil in seedling tray?
Pretty sure thats fluval stratum
I tried emailing the europe division of plant cell technology asking where I can get biocouplers here in the UK, no answer at all unfortunately. Since you are the man, could you try on your end to see what is going on? could be cool if rest of the world could actually buy some. thx
Hi, sure! I'll be helping you with the query.
I am forwarding your message to the concerned team of our company. Please hold for some time while I come back with the answer about this issue. Appreciate your patience!
Thanks!
Also, in the meantime can you please also forward your query to orders@plantcelltechnology.com
@@PlantCellTechnology cool thx I'll try that, I'm in no rush
@@RusticRaver Of course! Let me know if you need any other help!
Hi,
The team wants to learn more about you to help you with your order and the details of the distributor you tried to contact. Can you share them whenever you get some time?
But what is a biocoupler?
Biocoupler is the simplest temporary immersion bioreactor. It helps in the efficient multiplication of tissue culture plants by providing an optimum environment and nutrients they need to grow and develop in labs.
Read these blogs for a better understanding:
1. www.plantcelltechnology.com/blog/simplifying-temporary-immersion-systems-for-plant-propagation/
2. www.plantcelltechnology.com/bloghow-to-use-the-biocoupler-temporary-immersion-bioreactor/
It's a double lid with drain holes that can couple two jars together.🤪
@@capnsmashem1554 Simplest way to say that!