TISSUE CULTURE 101 | PART 3 | Making Initiation & Multiplication Media + Sterilizing with Microwave
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- čas přidán 14. 08. 2021
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Says "Because I'm lazy" as if on que, the smoke detector with a dead battery chirps XD
Hahahaha accurate🤣
I finished my masters in this field and used medicinal plant, I can say its easy for me but the only thing you need just make everything clean 🌷🌷
You are so awesome! I love how you talk about this like it's all easy and you don't have a degree so anyone could do it, but it's really quite a bit of science. You did all the necessary research, built your own (very well organized) lab, developed good technique, edit good videos and run a business. Fucking epic.
Thank you🥹
I've watched all your series and it's really helpful! I just want to say thank you for your amazing job! Look forward to see your next videos!
Thanks for the tip on microwaving the media. I thought I needed to get a pressure cooker or autoclave, this saves me time and trouble!
The microwave is a lifesaver!
Apart from being incredibly informative (thank you so much for that), this actually looks fun! You look like you really enjoy what you do!
Thanks for sharing! I'm about to start as well.
Another great video. Enjoying the series.
Thank you so much, Frank! I appreciate you!
The videos have been very helpful. I started my first batch today. I bought florida beauties from Alice. Two problems I had. One, separating all the plants. Some were difficult to determine where to separate. Second, the medium did not get solid, so I then added agar. I bought the MS with gellum. I thought maybe I didn't wait long enough. I keep referring back to your videos. Thank you!
Hey girl how’s it going can we get an update?
Thx so much for these videos- you explain everything so clearly and make it look simple - fantastic content,
Subscribed because your working on a beer pong table haha great info though
Thanks again ! I really appreciate your videos ! Waiting for the next ones ! :-)
Thank you for the great tutorial 😍😍😍
I built a tiny 10"x10" flow hood. Just enough space to pour plates and cut up explants.
Youre amazing thanks
You're awesome thank you😭
Hi,
Thanks for sharing.
For me, the most difficult part will be to sit and work on the floor which I can't do and was too hard to do when I was young.
For info: when the containers are hot and if you open immediately the microwave, the containers will collapse. And due to a big difference in temperature, more fresh air with bacteria will enter inside and you will get more contaminations.
That's why it's better to wait for the containers to cool.
I use a pressure cooker and I have less contaminations when I leave the flasks cool before opening and removing my flasks.
Daniel Jheelan from France
Thank you so much for your videos. They are so informative. I'm in Canada and Im So excited to get started. I'll be receiving my stuff to make the media today from phytotech and I'm stoked to give this a try.
I would really love to see some more videos, I have so many questions 😆.
Heyyo thanks for making these videos!
Yea girl
Thanks for sharing! Busy figuring out everything I need for my first setup at the moment. These have helped a ton!
Thank you! I’m so glad my videos are helping you. I wanted to organize an easy to follow series to help people get started. I know for me personally, getting everything started was so overwhelming and I want to help ease beginners into TC❤️
@@TatumSweet You are doing a great job of that already, keep it up!
Would you use the same media for plant tissue or is this specific to plantlets?
You're awesome, thanks!
what are the concentrations of the hormone solutions you are using? I'd like to create a solution from hormone powder
Hi Tatum! I enjoy your videos! Please, can you name the brand of the media you are using? I can’t find it on amazon thank you
@Tatum Sweet do you ever sell the media you make? I want to try to put one of my TC babies in the media to try to have them multiply. I heard you still your plants so you have an etsy store or do you do pop-ups.
Where did you buy the rack for your plants? I'm looking to buy the same one.
Excellent Video, very inspiring.
I have a few questions:
When you order the MS media, NAA, and stuff,
Are they delivered to your doorstep?
Do they come in special packing?
and after you use them, do you keep them in the refrigerator? Or just keep it at room temperature?
Should they be used with extra care?
Love all your videos. Thanks.
You'll have directions for storage on the media bottles.
Hello after preparing the medium if i cut a piece of plant and drop it in the medium will it grow?
Please answer me
Where do u preserve the hormone stock solution ?
Do u use IAA hormone?
What is Ba and NAA? Not listed in your Amazaon Lab
Hi. When the medium is cooled, the lid is wet with droplets of water. Is that ok to put the lid on as wet as it is?
I'm really enjoying the videos.
Wondering if you use a separate rooting media after the multiplication media?
Yes, there are different media recipes used for the two stages!
Do you use this initiation media for philodendrons?
Get a ring light homie. Thx 4 vids
Can I ask how you know to microwave it for 7 minutes? My microwave is 1000watts and I can’t find any info online..
You are great 👍 , if you have time please make a list of the things we need to acquire especially for total beginner like me. Please Tatum
She has a list of everything she uses for the lab, click the link in the description that says my Amazon lab
Can someone please answer , what do I need for the first media to make it grow , m&s , bap , naa , ? I saw also ppm? Please 🙏
Hi For making my initiation media do we need BA and NAA? Hi
Where do you buy the actual media and chemicals from? None of that is listed
Did you ever get that smoke alarm battery changed?
How do you know when to move from initiation into multiplication
Can this recipe be used for medicinal plant?
whats the concentation of BA and NAA 1mg/ml???
I'm looking for this all in MS+vitamins+agar, but can't find it. You have an idea where to find it? or what's it called so i can search for it? thx for the video!!
excuse me what kind of plastic container for heat resistant ?
Would you be able to help me with any info about the media for figs?
I need too take a lesson can you tell me where
Hi there! Thank you for uploading such helpful content!!
I just started doing research and wanne know: For each stage of growth is there a different type of media used?
That said initiation media is for the first stage and only needs ms?
Wounder same aswell someone answer please 🙏
I'm curious about this too
What is the temperature for doing this
Hello! Where do I find the pre-dissolved BA and NAA stuff you used?
It's in the description
Hi, thanks for video, Im a beginner so i have a lots of questions😊
- so the process you did here is for multiplication process not rooting process is that right? How is the rooting process done then?
- can you store the media if you end up making more than you needed?
Thanks
I wounder same aswell someone answer please ! 🙏
Pytotech have so many premixed, I am bit confused on there websites can you clearly mention the name of the ms medium
When do you wrap the lids with plastic wrap? After you add the media or after adding the plants
Congratulations! You have been selected as one of the winners for the giveaway:) Please reach out to me on one of my socials or email me at tatumsweet94@gmail.com so we can make arrangements to ship your prize! Thank you for your support💙
Also, to answer your question, I wrap them after adding the plants:)
@@TatumSweet omg !!! I will thank you
Hi , Where do you store your prepared media ? How long can you store it for
Wounder same someone answer please 🙏
Could you share the Philo protocol?
I just posted that video! Go check it out:)
Can someone tell me what the name of the Tissue Culture group on Facebook?
You don't worry about the microwave destroying the cytokines?
What actually is all the stuff you are using
Do you sterilize your deli cups before you use them?
Nope!:)
@@TatumSweet okay thank you!!
Im going tu start TC my self and your videos are amazing!!!
The videos are great but your eyes are better😛
just an idea, maybe remove entire carousel plate with all 10 containera at once. im gonna try it.
Interesting! Keep me posted on the results!
Change the battery in your smoke detector..
Idk what that is
@TatumSweet I guess more proof reading is in order before I comment lol
Haha all good! The battery got changed😁
Thanks for sharing yet again!! by any chance are you a zodiac sign Cancer? Thats what I am sensing haha
Haha I’m actually an Aries but cancers are my favorite🫶🏻
@@TatumSweet dang i shoulda known because im an Aries too! And we into the same hobbies
hmmmn making tissue media on a carpeted floor sounds like a recipe for disaster
Let’s put on our listening ears, friend!😃 I mentioned in the first 60 seconds of the video that the carpet isn’t my normal workspace and was only used for filming purposes:)
wearing gloves while wearing swimsuit. Lulz.
Very amateurish.
Yes, that is the point:)