How to ACCLIMATE Tissue Culture Plants!
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- čas přidán 10. 05. 2021
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In my experience, mixing a regular potting soil for cuttings with sand works well also. These options are a lot more sustainable than the peat moss. Normally I use regular trays with drainage holes in the bottom, fill them up with the soil-sand mixture and cover with a glass plate. I switch the glass plate a couple of times per day to vent off the majority of the moisture, and after a week (depending on the plant species and if plantlets have root primordia or not) start to leave a little bit of gap until you can remove the plate completely.
Also worth mentioning that plants from stage 2 (multiplication) are often not adequate for acclimation because of lingering cytokinins or strong synthetic auxins that inhibit root development. Not sure for the plant species you used in this video, but for a lot of ornamentals and woody plant species this is very important!
Thanks for making this video sir its very useful for me 😄🤗
Thanks so much!
Already wanting the updates
Awesome, more to come! Happy culturing!
Where is the store you visited located?
I didn't see you use any kind of rooting hormone. Is it not needed? If I were to take out my tomato plant out of the culture would I need to dip in hormone, then place in the perlite moss?
Super informative video! I was just wondering where one could purchase the tray you're using to acclimate these little guys. I recently purchased some Thai constellation monsteras that needs to be acclimated and I really want to keep these little dudes alive 😁
Hey, Thanks for your feedback!
I bought the trays from my local store.
Your working with my favorite group of plants! How do you know when the plants are big enough to be taken out of tissue culture?
Oh, that's amazing!
Plants are taken out from tissue culture after root and shoot systems are developed. First, they are transferred for acclimation, then to the greenhouse, and then can be transferred to the normal field or natural environment.
Hello! When the plant is fully acclimated to the humidity in my setup, how can I move it to soil? Can you please make a video about this? or just a reply would be really helpful, thank you!!
You can treat it as a traditional cutting, just keep the humidity high for a few days and slowly acclimate the plants to lower humidity, than you can move them outside to the shade and slowly move them to the sun.
+BONJOUR de france :) ! dites pour le transfere des plants tc , on peut utiliser de la sphaigne vivante + perlite ? ou on est obligé d'utiliser de la sphaigne "morte" deshydraté , compressé vendu en sac .. ? merci
How wet do you need to keep the medium when you’re finished?
Can you elaborate on your question a bit?
Just wanted to ask, is it necessary to wash the plants from the agar? I saw you mainly just removed the plants and then placed them on the media. Just want to find out if I can just remove as much excess agar without washing them? Great video by the way
It's always recommended to wash off the agar from the roots of the plants before acclimatization. Further, an attempt to remove agar by hand or forceps may cause some damage to plants.
What was composition of medium for TC of Pinguicula?
The tissue culture media 1/3 MS with 25 g of sucrose, 1.0 ml/l BA, 0.1 ml/l NAA, 1 ml/l PPM, and 3 g of gallan gum.
for the acclimation, 50-50 sphagnum moss and perlite.
Hi! Love your channel! Quick question, I'm currently acclimating some philodendron white knights. I'm at the point where my pwk are staying uncovered all day and all night. The process of acclimating was slow and I took my time and did what you suggested. My question is, is it normal for some plants to have the occasional yellowing leaves? I've been cutting them off, is that okay? All the leaves that yellow are always lower leaves, does this mean anything in particular? Thanks!
One of the reasons for the yellowing of leaves can be moisture stress (either too much or too little water).
@@PlantCellTechnology thank you so much! That is what I suspected.. I transferred them into their nursery pots yesterday and found the soil was pretty dry even tho it looked wet, it's tricky when you use alot of perlite.
Do you every add co2 to the tissue cups before closing them? Does that help them grow faster adding co2 gas? :)
Roots breath air, you are trying to develop roots. It’s the hardest part of the culture process in many cases , atmospheric co2 is more than enough
Do you have any videos that demonstrate meristem culture for eliminating viruses?
Hey Jason,
Currently, we do not. However, it's on our list and soon we'll create a video on the subject.
Hi! I bought some cephalotus and vft tissue culture online and I'm having lots of issues with mold during acclimation. I soaked and rinsed the tissue culture multiple times before transplanting so I don't think that is the problem. Do you have any advice?
Should I try sterilizing the media and soaking the plants in anti-fungus next time? Maybe I was keeping the media too wet?
I was also wondering if you have any losses during acclimation and how often.
you can try to makle new cuts after washing, using clear vegetal tissue extracted from the internal part, making cuts with a sterilized sharp and putting itronto your media
Did you ever figure out the mold issue during acclimation? I’m about to acclimate mine and am trying to gather as much info as I can ahead of time in case I run into issues. Thanks! 😊
@@mariapennylane I tried to be even more diligent with rinsing, while it's in a clump, while I'm separating into individual plants, and while I'm transplanting. In my experience, VFTs are just so much hardier than cephs. I haven't had much trouble with VFT cultures but still have not had great success with cephs.
Any concern that the moss or perlite has any type of bacteria within it?? I'm on the paranoid side so might consider using an ounce or two of hydrogen peroxide/water with the media in a gallon baggie....and shake...then maybe rinse with distilled vinegar.
Is this a legit concern...or overkill?? TIA
Use some fungicide to control any fungus or bacteria after deflasking.
Hey, what about fungus and agar🤔(root fungus cos of agar)
Fungal overgrowth in acclimation is probably more due to leftover sugars in the media that stick to the roots and the base of the plantlets. If you acclimate rooted plantlets make sure to wash out the roots properly in water before planting.
Keep it up brother 👍
Is that moss and perlite ? Why kind of moss?
Yes, right!
💙💙💙
I want to take class can you tell me where
You can go to any college teaching tissue culture or plant biotech. Or you can search for organizations that provide courses on the theory and practices of tissue culture.
Is tissue culturing a plant the same thing with cloning the plant? There is no genetic modification (GMO) is involved, correct? Some people sanitize and do this tissue Culture different than yours. I'm not sure what makes the difference?
No, cloning and tissue culture are two different techniques. In cloning, identical organisms are tried to be produced, however, tissue culture can be used for several other purposes, including enhancing plant productivity, obtaining disease-free plants, conservation of plants., and even achieving identical plants as well.
Tissue culture is a sum of many other techniques, in which similarity is culturing some plant tissues on a suitable culture media under lab conditions.
Tissue culture is simply a technique to grow plants in a lab environment for specific purposes.
In cloning, genetic engineering is involved. And, these cloned cells are often needed to grow into plants using tissue culture.
There's no genetic modification in tissue culture artificially unless you combine this technique with genetic engineering techniques that are used to modify organisms' genomes.
In this video, you are watching how plants are acclimatized to be transferred to a normal environment from the lab environment. So, it's the last stage of the tissue culture, that you are watching in the video.
Before acclimation, sterilization, culturing, multiplication, and rooting steps are followed.
I hope you've got answers to all of your questions.
Happy Culturing!!
I wonder even when you have >1.5k subscribers, you have views in less than 100?
🙄🤔
We have an excellent community in the tissue culture space. Our viewers are very engaged and want to learn about proper cultures. Best of luck with yours!
@@PlantCellTechnology wow thanks for the reply. Can I contact you regarding some issue with endophyte contamination? Thanks 😊