Tissue Culture Plants Basics & My First Experience Rehabilitating @Rareplantstissueculture
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- čas přidán 30. 06. 2022
- In this episode, I share everything I recently learnt about rare plant tissue culture. Just what is Tissue culture? How is it done? How will it shape our future market? And how do you even create variegated plants with tissue culture? I answer all these questions AND MORE. At the 2nd act of this video, I show my rehabilitation process of these plants. There's a lot to learn in this episode!
Supplier instagram: @rareplantstissueculture
My instagram: @bo.tan.ist
Support me on patreon: / seanfromonlyplants
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19:20 - I was just like "nooo, stop it!"
I've personally had zero issues with TC Thai Constellation babies, but I always only took them out of their jar when they had quite a good amount of roots. That into moist sphagnum moss and a cup over it for high humidity. So far I've had 100% success with this method.
They might have been damaged due to inconsistent care…
Hey, I have not worked with those specific plants, but I run a tissue culture diagnostic lab, I mostly culture viruses/viroids out of plant strains. You might have more luck with the survivability of the plants if you harden them while they are still in the original jars at least for a few days. I would start by just cracking the lids just a bit.
Our method is using Rockwool instead of moss, I take my rooted babies out of their culture tubes and rinse them with sterilized water. They then get placed into rockwool inside a little growing tray that has a vented lid, for the first week we open the valves halfway. 2nd week open the valves completely. If they look good slightly move the lid off over the next week. But our cultures take longer because we propagate from callus culture to a full plant from just meristem tissue. My babies need lots of hardening. I also in some of the plants we work on dark root hardening, either put them in an incubator or warm area covered from light then slowly increasing the light, but those babies look old enough to take direct light. If you want to increase root strength it is an option.
I am also concerned that one of those jars looked contaminated prior to opening. Normally a lab does all the culturing in a sterile environment using a laminar flow hood, there shouldn’t be any fungus or mold. Even if you are culturing from explant and not like what I do with meristem you sanitize the plant before use, no mold should be present in that magenta jar.
It doesn’t look like you plan to tissue culture, but if you want to save some of your babies from mold you could mix up some antifungal. Our labs orders from fisher scientific and Phytotech . I would recommend phytotech for when you are first starting out; look for Product ID: A119 Amphotericin B or Product ID: C1830 Crystal Violet. I would suggest going with crystal violet first it is less likely to shock your babies. If you need a recipe, feel free to msg me and I will send you mine.
Omg thank you for taking the time to share this. I am a curious observer of Tissue culture and will always bring it up on the channel as it is the future of houseplants. But I dont think I will be doing any tissue culture myself, nor will I consider buying them in jars for the foreseeable future (too busy to deal with the process 😅). Thank you one more time for the tips!
@@onlyplants actually TC is not new ,just house plant started late , been a long time for the aquascaping trade , what odd is we are not taught to acclimate it
Very interesting.. was thinking about buying a spiritus scanty.. but glad I watched this bc the reg plant is about the same price as a TC.
I am happy to see great looking product from that seller, I am currently waiting a first shipment and this is huge peace of mind!
Whoaaa good luck!
Rot/damping off is very common in TC babies and seedlings in general! The high humidity environment they need allows bacteria/fungus to spread rapidly and the lil plants are super weak, so you wanna keep everything as clean as possible early on in acclimation. A seller I purchased from in the past recommended incorporating a fungicide + B1 vitamin bath for the plantlets after taking them out of their vials. Can't wait to see more TC videos from you in the future!
I also use a fungicide to clean my explants after I deflask. Very helpful.
Shouldn‘t there be a last change of the medium to one that contains hormones to encourage root growth?
Sometimes it’s a PH issue, there are tricks to fix this.
Thanks for the tips that makes a lot of sense!
Sounds like it!
Thanks for being our tissue culture mafia guy on the inside.
😀😀
⁹⁸9⁹⁹9
THIS is what I’m currently into. I’m slowly feeling my way through it, and it’s amazing you included us in this next venture of yours!
CANT WAIT FOR ‘IN LAB’ CONTENT 😭🥺💚
Thank you!!
Hehe will be visiting some labs soon.
20:00 step 1 of appreciating tissue culture specimens is shock going from 100% humidity & sterile environment to atmospheric. Best thing you could have done is to keep humidity high w transition (not yanked & dropped on spagnum)
Thank you for this
@@onlyplants I was not being flippant and have forgotten what you were doing exactly. Just think of yourself unable to handle temperature change of 50F (naked) for more than 1 hour. Well that is your mammalian interpretation. What if you leave shower at ~90F, ~100% RH and step outside and back into bathroom that is (assume proper fan operation)…~65-70F, ~50% RH. You assumed that is fine because you think like human too smart to think like plant. Minutes crash 💥 plants if you do not have them at right state to stage 3 (a plant in pot or landscape like any other).
21.10 - the lab should have told you how to rehap the plant. from what I see here, it seems too hot and you mentioned that you open it every 4-5 days, it should be in that plastic cup without interruption for a month.
After you took them out from the jar you have to clean all the gel from the plat and then submerge them in fungicide solution for a moment, longer if the plant has been contaminated the stick them in you planting medium(I normally use peat moss ) then leave them in my grow tent for a month. I did this with thousands of TC plants and they are fine. Hope this is useful to anyone reading.
Im taking notes with your advice, they are very sound. The fungicide solution sounds like a good idea
Would you provide some information on a fungicide solution? Is it home made or store bought?
@@sarah6479 any store bought is fine, just don't forget to wear gloves when use them.
Add a few drops of betadine is also a good idea, it helps with bacteria problem.
@@SkyPlants ty ty very much!
This is so interesting! Hoping to learn even more about tc
Seems like the next gold rush 😅
In orchid societies, mericlones are highly sort after and sell for more than seedlings.
( in Australia, the university of New England offers a one week course every year on the latest techniques.)
Cool! I also hear this is the best and easiest way to import plants into Australia.
@@onlyplants to my knowledge it’s only orchids , other plants have to pass bio security laws.
26:47 need to focus on keeping humidity high and rest (low indirect light). Not all the atmospheric screwing around (cover instantly with hermetic cover & progressive acclimatizing)
I started collecting my wishlist philos specially rare ones through baby TC and they're all doing well.I never watered them since the day I acclimated them(an squeezed moss is enough) after 1 month of acclimatization I slowly introduces them to the outside world🇵🇭🇵🇭🇵🇭♥️♥️♥️.and they're the toughest one once they're used to your local temperature.I have grown one and they're expose to direct sunlight and never experienced burned leaves🇵🇭 sometimes had 40°c burning hot here 😲
Thanks for the share! I have a feeling I didnt care for the jars consistently/correctly causing the plants to weaken
Amazing!!! 👍😍
Tissue has a long way to go before the consumer receives it . If they are selling tissue culture that small then the price should be much lower. It’s too much of a gamble for the average consumer . You , an expert is even having trouble .
Yeahhh, but they are easier shipped this way as they can pass phytosanitary test much easier. Also no more packing etc
@@onlyplants exactly
The point of the meristem is actually not only that‘s capable of cell division. It‘s also that these cells are totipotent which means that there are able to form every kind of tissue e. g. leaves, roots etc.
Oh yes, heard that in bloom and grow radio podcast😀 its a sexy word actually totipotency
So informative i liked.
Thank you 😀
SOOO COOL!!! 😎
I have some pink princess tissue cultures. When I took them out of the gell I potted them in regular potting soil in small paper cups, only lightly watered, and placed in a plastic zip lock bag. It's been 5 days and they are good so far. I wonder if your plant was already sick before you planted it. I believe if your tissue culture was really sterile, the gell would not have molded.
Its possible they were contaminated. Some suggest to spray fungicide when repotting to sterilize it. I should have done that 🥲
I just got my first tissue couture Thai constellation and I’m nervous because I only have I try . Tfs wish me success . ❤
Fungicide spray and slowly introducing to our environment air seems to be the key
Wowwww!!
I grow big my caramel marble, vat billi ang joepii from tissue culture but i bought them with moss already. But the moss is placed only at the root area not touching the leaves inside plastic cup with cover.
Whoaaa thanks for the share
I have no idea of how to handle tc... but eventually you will conquer this.
Takes killing a few to learn
selling plants is a gamble and in deciding which plants are going to be hot. it's okay to flood the market, why should a select few have access? they already made a lot of money and it's all part of the game
❤️
I think it is good that there are a number of plants that have a higher price. As with everything a limited amount of something makes it special. Not that common plants are not special but they are less "intereting" for lots of people. Every kind of merchandising usually has a group of things that are cheap and a group that is more expensive. That is what keeps the market alive. People can enjoy common plants but should have the option to get some more prestige/rare/expensive plants
Correct. It works the same with fashion
My background is plant physiology & tissue culture. Ironically, I am just starting to leverage in U.S. Would love some production contacts in Thailand. First year will be a few thousand each of existing lines TBD.
Cool, this ones a good contact but theres hundreds maybe thousands of good tissue culture labs in Thailand
@@onlyplants wholesale only or good transition quick from market check 6 month sample quantities & quick 1-10k? I am not talking balcony botany (albeit entirely legitimate idea & highly encouraged…just sounded authoritative & less pejorative when I first wrote it
Hi i wanted to ask, if anthurium silver blush frequently blooms (infloresence)? Wether or not its good for pollination? Thanks in advance:), great content
Its actually used a lot in hybrids as parents. This suggests that they are quite good at blooming (and have the desirably traits people want for hybridizing)
After placing the clones do you close tight so that no air can go inside your clones, asking
Yes. No air should go inside. The humidity in there will automatically reach near 100%.
Im not an expert in propagating plants but I do have a feeling that they should thrive better in water?.. idk because in my case water prop is the safest methos
The media/gel doesn‘t only contain nutrients, it also contains hormons, pH puffers, antibiotics, and other substances to keep the conditions optimal for the young plants. Therefore, it won‘t work to place a tiny piece of a Philodendron‘s stem without a node and a roots in a jar filled with water and grow it into anything. It would simply rot due to being exposed to pathogens and other harmful factors
If they didnt do a mutation experiment to create a new hybrid with the TC approach, why would they take such an effort by doing it this way to propegrate?
TC is way faster and cheaper to propagate than even seedlings 😀 some plants also do not produce seedlings easily.
Philodendrons for example are notoriously difficult to pollenate and set seed
Hi Sean. Will the plan lose their variegation after grown up?
Mine did lose variegation 😅 but it depends on your care, environment, and luck. With a few exceptions, most (98%) variegated plants will revert in low light
You need to keep everything sterile until it gets a really good root system. Taking it out and putting it on that counter and not sterilizing your hands etc probably contributed to the first one rotting...
Wooh that makes sense. Some have commented to give it a fungicide bath immediately, which makes sense
What is the medium you use to feed ??? Thank you
Im not sure about the TC producers medium… most of them might keep it in industry secret 😀
@@onlyplants what do you use ? How much sugar, ms basal medium, kinetin, and agar? Please :)
@@onlyplants nvm I got one of the top companies to tell me. Thank u but no thank u.
Now why would they sell them in this TC jars? why push the risk of this rehab/acclimatization to customer?
Cheaper to ship, no packing fee, and also easier to get phyto approved. Sending plants bare root can also have some risks… i think either way its best to buy established plants in local plant shops rather than stressing out with importing risky plants (at least thats how I would view it moving forward)
@@onlyplants it's always good to experiment Sean... I'm thrilled to see those cute plants thrive & grow.. .
Also sad to see that first plant lose its leaves. hope it recovered.
I am currently acclimating Joepii, Billietiae, and Ring of Fire. All 20 of my White Princess died.
Oh mannn thats a lot of deaths. Look like we need to master acclimatization
@@onlyplants or maybe replating is needed.
They died because of my negligence. They melted because of too much heat.
@@lonestartropicalstx I wouldn’t worry too much , white princess have already lost there value. A$10 each in my part of the world.
Market forces will always dictate the prices .
Yes 😀
First yah ,,,,,,!,,,,
❤️❤️
Hello, do you have experience in palm cultivation?🌴🌴🌴🌴
Unfortunately no 😅
hello, I make this message in order to know if someone has already ordered with this company or to know if it is reliable. thank you for your feedback
I hope someone in the comment replies to this 😀
Link pembelian tanaman kultur jaringanya ada om?
ada instagram nya kak di video description
Avez vous acheté ?
Cute Plants ^^
Like 86
MY friend, have a good day
Thank youu
This seems way more difficult than putting a cutting in water 🤔🤷🏼♀️
Yesss haha and I enjoy water propagation more. But with tissue culture you can clone thousands of plants in 9 months.
I digress lol I would be so discouraged losing even one 😂
Can you visit our company
DM me on instagram
@@onlyplants where is your Instagram page
Oh no! Treating plants as disposable and replaceable yearly is terrible from an ecological and carbon footprint perspective. A good balance is needed.
Hahaha!
You’re right but no one cares.
If you’re in the US then try this as an experiment in horror; go thru the garden center at least 3 times per week, take note of the plants they have, mostly look at the more tender or sensitive perennials but many of the annuals and houseplants too. Then on your way out drive past the dumpster and take a peek inside, employees may tell you no, so don’t ask, many don’t care, don’t fret, it’s not illegal or anything. They usually have 2 dumpsters, one that stays just outside the employee door where they bring soil and plants inside with a forklift, that’s the dumpster they throw things away into all day, then they’ll take that smaller dumpster and dump it into a bigger dumpster, it’s usually all the way around back.
You’ll certainly see MANY plants!
Again, just take note of what you see in there, (you’ll be shocked!), then repeat these steps as often as you can, to get a good picture you’ll want to check a few times a week.
By the 3rd trip you’ll almost certainly see rhythm… Lowe’s has policies that often aren’t followed. They are supposed the dump out the “trash plants” meaning they should be removing the pots and save them to return to vendors instead of sending plastic (and fancy ceramic pots) to the landfill, there should also be another dumpster for “organic material”, meaning they’re supposed to dump the dead plants and soil into a separate dumpster so they’re not sending that stuff to the landfill, it should at least be used to make mulch, it’s a waste of landfill space but it’s also a massive source of methane production.
In reality most Lowe’s don’t dump the pots, they simply pick out the “dead plants” and throw them into the landfill dumpster, many of the plants are fine, just struggling due to poor care and just need some water, or less water, or more shade (they love to cook plants that prefer shade).
Anyway in most cases I’ve found that they just throw the whole thing away!
They don’t dump the soil, they just grab the plant and pot and throw them away all together. With the more fancy houseplants they are often growing in a plastic pot but they place that pot into a fancy and very heavy ceramic cover pot. Those cover pots are usually shipped from China, theyre almost always shipped from somewhere overseas, and theyre rarely a shape that can nest into each other, so they take up a ton of space and require lots of fuel and resources to make and ship… and in that dumpster you’ll find that they don’t even remove that cover pot! They have similar pots for sale on the shelf for big bucks! They’re not cheap!
But Lowe’s can’t resell even those cover pots since there’s no CPU in the system for them…so to Lowe’s they’re just trash… and that’s how they’re treated…. Plant, soil, plastic pot and ceramic cover pot just tossed in a pile and dumped… it is appalling!
The plants are usually not dead at all, the soil is just fine, and obviously the pot and cover pot are just fine too… but they’ll just pick up the whole thing and send it to the landfill.
I’ve been “kicked out” of my Lowe’s 5 times this year! I don’t care, I barely pay attention.
There’s often so many plants that I’ll fill my vehicle as full as possible, race home to unload it then race back to gather another load… often I end up leaving way more plants than I take just because I can’t use them for anything.
This is just regular day to day stuff at most chain box stores.
The waste created is incredible!
So much human labor was required to get that plant to the store in good shape, but the resources wasted is beyond comprehension.
Peat most isn’t really renewable, most soil mediums aren’t really renewable, the plastic is simply a byproduct of the oil industry, they “need” to make plastic stuff, otherwise that byproduct would just “go to waste” since we don’t have other uses for it (that’s not true, it’s just an oil industry lie), worst of all is the a tie between the the Chems like pesticides and fertilizers the greenhouse industry relies on, they use ALOT! But fuel is likely the worst waste involved! All the materials (soil, pots, Chems, and even the plants themselves) get shipped to the greenhouse, a lot of is shipped on container ships then shifted to trucks to get it delivered , they then grow the plants out, after that they’re loaded back in a different truck and shipped cross country, then the garage truck gathers up the dumpsters and drives it all to the landfill.
Truly it is the circle of life, death being the biggest part of life.
It’s disgusting! I do not buy plants!
I either dig them out of the trash and rescue them, or stroll through the garden center with a razor and take cuttings, jam them in my pockets or purse and riot them up at home.
My conscience guides me, I lose sleep over all that waste I described, but I don’t feel a little bit bad about having hundreds, maybe thousands of plants under my care that I didn’t pay for.
I think if the public knew about that waste, a few would be concerned, but only a few… if they knew the impact, I think the practice would be made illegal but that is not the world we live in!
If we keep this up there won’t be a world left for us to live in, or our kids… THATS WHAT REALLY KEEPS ME UP AT NIGHT!
Cheers
Oh my god that sound horrible. We don’t practice this kind of large scale commercial growing here Indonesia but I can see how that can become irresponsible and unsustainable.
There are ways to do it sustainably, that would beat buying plastic plants or cut flowers. I would love to explore more in this channel soon as I move into my new home. Styling is my passion, and so is sustainability! Thank you for the reminder
12:37 gatekept?
Yea kaylee Ellen speaks about this a lot in her videos too
Gatekeep gaslight girl boss plant industry
@@oliverpiong6558 yup. Companies are greedy. Nobody is in it to actually get people plants. they are in it for money.
@@oliverpiong6558 maybe eric c is a girlboss....
😅
There’s absolutely a right and wrong. Plants are in nature. There is no way it should be come an elitist, rare, “hard to find” capitalist game.
I hate to see that because these plants are indigenous to their areas and simply harvested by people who have the means to do so. It’s not fair or ethical to hold the cards on them and charge prices that even people on the land where they are forested couldn’t afford it. That’s so sad to me. I don’t like variegated plants as a personal plant lover and sometimes that’s a good thing because I couldn’t afford them anyway. And I also refuse to play the “rare plant” game. There’s a dark side in ever community I guess.
It is actually more complex than that.. while some rare plants are found in nature, a few of them were actually selectively bred, or hybridized, or tissue cultured to achieve a physical character that cannot be found in regular plants. This process is often one in a million and could take years to master.
But looking at it historically, all rare plants eventually become common plants. There’s a cycle… it is advisable to simply wait for prices to stabilize. Its also perfectly ok to admire plants from afar. Rare plants that cost a lot actually brings anxiety and stress sometimes. 😀
I guess everyone have their own views and relationship with rare plants. I would only keep a few but I actually enjoy my common plants more. 😛
@@onlyplants I appreciate you replying to me. I love your channel so much! I agree with you that if a plant is bred then yes absolutely charge for the care and creation. That’s something different entirely, you are right 💚
I don't know why I'm so amazed by people interested in artificially created crippled plants with missing chlorophyll with no benefits by all means. It's mind blowing to me.. I would totally get it if the plants would be somehow actually better or superior to the natural ones, but now it's just all silly. waste of money, waste of materials and human force while creating these. this market distorts economy in my point of view. there are so many regions all this knowledge and money could do good in the human existence (...). But yeah, I guess it's all subjective to judge, since we're living in an unregulated capitalist world. sorry if this sounds negative. this is the first video from you I actually don't like. though I did learn something.
Every time I see some expensive variegated plants I'm reminded how ****** humanity is, lol.
This is a controversial and complicated subject matter that has a wide spectrum of debate. I am neither for, nor against it. This technology has pros and cons depending on how one sees it. I am merely an observer and story teller 😀 and I love to see people have conversations on this so thank you for the time to share. ❤️❤️
@@onlyplants we're all here to educate ourselves, nontheless ❤ and I totally get your neutral position on the topic. though, I wish it wasn't so 😅however, we're all greatly appreciate your insights and reviews, of course!!
best .)
This seems really shady and corrupt lol
Very.
lol. Its a bit unregulated and competitive