How to Tissue Culture a Nepenthes Carnivorous Plant with
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- čas přidán 6. 07. 2024
- This has got to be one of the most requested videos on our channel: How to Tissue Culture a Nepenthes Carnivorous Plant ("Pitcher Plant")
This video is a carnivorous plant tissue culture collaboration with @plantsinjars ! We filmed how to tissue culture a venus fly trap carnivorous plant over on her channel, which you can watch here: • DIY Tissue Culture: Ca...
It is good to note that this process applies to most carnivorous plants, so feel free to venture out from just nepenthes and tissue culture more of your carnivorous plants!
👉 Save 10% off your first purchase from the PCT store to get the products you need to tissue culture: www.plantcelltechnology.com/?...
And if you're thinking, "This is awesome, but I want to learn even more," then you're in luck! We're hosting a master class that's literally designed to turn you into a tissue culture PRO in just two days. Check it out and sign up here: plantcelltechnology.com/master-classes/
Time Stamps:
0:00 Hello from the PCT lab
0:59 Making Carnivorous Plants Media
7:45 Explants Sterilization Materials
13:17 Cutting the Nepenthes
20:39 Nepenthes Explant Sterilization
21:33 Nepenthes Seeds Sterilization
26:33 Pouring Media in Petri Dishes
29:39 Laur Preparing Media Vessels
29:54 Inducing Nepenthes Tissue to Media
37:18 Planting Nepenthes Seeds in Tissue Culture Media
37:35 Inducing Nepenthes Leafs for Callus Formation
38:02 Final Thoughts
Don't miss out on all the fun and learning. Follow us on our social media channels for more tips, tricks, and plant science magic:
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🛒 Products We Used in the Video:
- Supreme-Grade Agar: bit.ly/3T4nZy3
- MS Media w/ Vitamins: bit.ly/3YPAaAp
- PPM™: bit.ly/3ZLhXES
- Gellan Gum: bit.ly/421HWto
- IBA Solution: bit.ly/3ZysQKA
- NAA Solution: bit.ly/3LsMP9p
- BAP Solution: www.plantcelltechnology.com/b...
- TDZ Solution: www.plantcelltechnology.com/t...
- Kinetin Solution: www.plantcelltechnology.com/k...
- Square Media Vessels: bit.ly/3J7yigg
- Round Snap-Lock Containers: www.plantcelltechnology.com/r...
- Biocouplers™: bit.ly/3J3aD0r
- Biocoupler™ Glass Set: bit.ly/3mHm6v3
- Biocoupler™ Bundle: bit.ly/3Jui3eR
- Laminar Flow Hood: www.plantcelltechnology.com/2...
#planttissueculture #plantcelltechnology #tissueculture #micropropagation #plantscience #tissuecultureplants #tissueculturemedia #nepenthes #nepenthestissueculture - Věda a technologie
Started crying at 14:35, but so happy you two made this video! I love both your channels and PCT store!
We are in awe! Thanks so much for your love and support!
Cutting the plants always tuggs at our hearts, but the good news is, you will get many more after tissue culture! Thank you for the kind words!
Thanks, realmente great.
Such a wonderful video! I absolutely love the advice. Would this sterilisation method for the nepenthes also work for staghorn fern spores by any chance?
Great Job Thank you both.
Thanks!!
Thank you. Great video!
Thanks. Happy to know that you find it useful!
I love your videos, i am a fellow plant and fungus lover. I culture fungi using some similar methods and i am moving back into plant tissue culture. It is amazing all the things we can do with agar and different types of sugars. Have you guys tried light malt extract for the sugar source? How about potato or rice water? Im cery interested to know
How much sugar would you recommend for a 20ml solution of diluted hydrogren peroxide? for helping with seeds germination
Your channel is great! I've been wanting to get into tissue culture so badly. I'm obsessed with plants, not just house plants but every little thing growing. We went to visit my wife's family in Philippines and I spent a ton of time just looking at plants. I'm gathering lab equipment, but I need a laminar flow hood. Im going to build my own, and I am debating on adding a bottom floor with holes, and an attached fan chamber to pull vapors down and out of the house through a vent. Are there any chemicals that have dangerous vapors in tissue culture, to warrant my concern? Or, would I be better off not adding the suction. I could see where the exhaust vent could suck contaminated air from the window that you put your hands through and into the sterile area of the workspace.
Why don't you give a try to our convenient portable laminar flow hood instead?www.plantcelltechnology.com/the-mini-portable-laminar-flow-hood/
This is a video I’ve been wanting for a long time 😁
We were thrilled to finally add it to the channel!
Love the channel. Learned so much and had some nice results. However, I don't have time to keep up with the hobby for now. I have a set of biocouplers (never used). I am in the dmv area. Can I donate them for someone wanting to get into tissue culture? Love what you guys are doing, would like to give back.
Thanks so much!! It's great what you want to do. Where do you live? We will communicate with our marketing team to see if we have any enthusiasts who are limited by budget but eagerly want to try tissue culture. We can help them with this donation of yours. How does that sound to you?
How can I dispose of the tc media?
I have been wondering this for a week or so, but would it be possible to sterilize the plants in a Sodium dicloro solution put in a vacuum chamber for a short period of time? or would the water in the plant just start boiling and kill the plant instantly? I thinking you don't even need full vacuum, just enough to break the surface tension. I know tween-20 is used for the surfactant but i was thinking a vacuum chamber would help also with getting the sanitizer into every crack and crevasse of the plant. food for thought I guess...
🌿🍀Thanks♡🙏🏽☘
Happy to help!
Can another cytokinin be used in place of TDZ?
Francisco uses TDZ for callus induction.
What species have you been able to get into tc from tissue? I heard that nepenthes, like the highlanders, have an internal bacteria that make them hard to place into tc from tissue. Also would they have to have a nighttime temp drip while in tc?
Nice video. where do you located? I want to get some information on tissue culture.
We are located in Washington DC. What do you want to know about TC?
@@PlantCellTechnology want to get information on if we can do tissue culture for curry leaf plants at home?
@@kasunrphoto You can do tissue culture at home. But, you need to have setup, clean environment, and a working protocol for the plant you want to grow in vitro.
I need update on this
Sure!
Thanks a lot and please update results! I hope they will be all successful👍🙏
Absolutely! Thanks for your love and support!
I've been watching you a week and already have things ordered to get started. However, I ordered a cannabis starter kit, as it was $99, and that was all I saw. Can I use that to get started with other plants, then move them over once the other media is here? I live in a rv now, planning a homestead. Do you have any advice on keeping plants around for a long time?
Hi,
Great to hear from you!
Before we suggest anything, can you tell what the kit actually contains?
@PlantCellTechnology it has a complete cannabis cloning mix, and multiplication mix. I've been looking at your channel, and I've come up with yes. I have other media materials on the way. Got a little over zealous and ordered plants too. I was checking in case anything came early or with issues.
@@FarmerShawns This is great! You seem super excited about your experiments.🤩🤩 We hope they turn out great. In any condition, do not neglect to maintain an aseptic environment for your cultures. The health of your plants depends on that, too. And, if you need any help, let us know!