Tissue Culture 101 - How to Acclimate Monstera Thai Constellation Micropropagation
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- čas přidán 21. 07. 2024
- Here we go over Tissue culture and the acclimation process of Thai Constellation Monstera.
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In this video we go over:
How to Acclimate Tissue Culture Plants
What is Tissue Culture?
Tissue Culture for Houseplants
Micropropagation for rare plants
0:00 Intro
0:10 Tissue Culture is Micropropagation
0:30 Philodendron Heartleaf Variegata Tissue Culture
1:00 Pink Princess Tissue Culture
2:00 It's important to gently acclimate tissue culture plants
3:00 Use a sterile cup with Stratum and Perlite
4:00 Lightly water with a squirt bottle
4:38 Thai Constellation Tissue Culture Damping off
5:30 Healthy Rooting Thai Constellation Monstera Tissue Culture
6:00 Mist Tissue to maintain humidity
6:20 Gently acclimate baby tissue culture to grow room humidity
7:20 Slowly increase the amount of lights on your tissue culture plantlets
7:49 LECA A+B Nutrient should start low dose
8:16 Eventually transplant to Aroid Mix or LECA / Perlite
#TissueCulture #thaiconstellation #micropropagation
I do earn a small amount money from amazon affiliate links if purchases are made (and I mean pennies). This does not create incentive to make videos like this. My goal is to make reliable, honest, plant content viewers can trust. - Jak na to + styl
Replaying this video on repeat till my white wizards arrive 😬
😅😅😅
so cool ! I am bachelor in biotechnology I love TC being doing it since 2010, got my own lab nowadays !
Thank you for your video! I’ve ordered some tissue culture and watching your video makes me feel like I can succeed in acclimating them!
😄
Thank you so much for this video! I got a Monstera Thai Constellation Tissue Culture and I needed to see this before I made my purchase.
How is your culture/plant doing? I’m looking into buying one.
Just received mine today! Thank you for the info 🥰
Absolutely! Glad you found it helpful
Thank you for the video! I bought a philodendron white knight and didn’t do my research so I didn’t realize that I was buying a tissue culture. Opened the dome the first day and leaves started dying so I found this video and realized what has happened. Managed to save it and now a couple months later I’ve finally worked up the nerve to start transitioning it to ambient. So far so good. Can’t wait for the Albo Show to make it to San Diego!
Awesome!! Sounds like the White Knight is doing great! Nice work! Yes we’ll be in San Diego on Nov 13th-you can dm us on instagram or text us to get on VIP! Hope to see you soon 😄
Aww that’s brutal the seller didn’t tell you!! I just bought a Thai constellation tissue culture I have to acclimate also
Thanks for this excellent explanation and process of Tissue culture....
😄
Love your channel! Curious where you found the "recipe" (protocol) for monstera tc mediums?
Do you have better results with the 50/50 stratum/perlite mixture than with stratum alone? I’d imagine the perlite provides better drainage and aeration but that’s just a guess.
I just bought a tissue culture Thai constellation yesterday! Thanks for this video! Luckily the seller told me I have to acclimate it! Lol
Awesome!! Good luck with those little ones! Keep the humidity high and scale them slowly into your growing space. We have a care guide coming out on the Thai Con at 10am today too!
Where did you buy one?
Do you rinse the stratum first?
Is copper fungicide a good option? Copper octanoate? Va mancozeb?
Thanks for this info! My thai constellation is my first attempt with a tissue culture, and I'm nervous, but hopeful!
Absolutely!! Good luck with it 😄 Make sure to watch our newer video breaking down the damping off / fungal issues that can affect it
@@prettyingreen will do!!
@@ckandrewhow did it turn out? Getting one today and I’m nervous
@@akarimian1 it was rough... But I'm not knowledgeable enough, I think, to have started with tissue culture.
How about pumice instead of perlite?
My Thai’s roots are rotting how do I get it to grow more roots can I do this?
Thank you Thank you for this info,
You are so welcome
This video is great on explaining tissue culture. I just ordered a TC tissue culture off Etsy. Wish me luck 😅
Wooo!!! Good luck with it. Make sure to keep all the parameters in range (temp, humid, water content in media, nutrients). And if you lose 10-20% that would be considered “normal” in my experience
@@prettyingreen thanks for the tips! I think it’s only 1 culture so no room to mess up! I ordered your leca kit so I can use the nutrient solution included in it. Hopefully everything arrives at the same time
@@tyrajames how did it go??
@@diamondprincss02 the plant has died :(
@@tyrajames oh no I'm sorry to hear that! 😢
Whats that 20% AB solution ?
I recently had my first "very lazy" attempt at doing a big batch of tissue culture aquarium plants for my fish tanks and about 95% failed / were assaulted by moulds and fungus😭🌱👨🔬! I really misunderstood the assignment, you're really personally responsible for creating a sterile environment for the plants to grow 100X faster than they typically can outside, but, you can't slip up at all with sterilising equipment or shocking them with big changes in temperature or lighting! However it's very much worth it when you get it right, you can multiply thousands of plants from a single leaf once you know what youre doing!
I can relate to this so much! I’m on about my 8th batch of TC and it’s very challenging to get the sterilization correct. I also lost all of the first few batches to contamination. But now I’ve had a few 80% success rate runs which is encouraging.
Agreed it is an incredibly difficult but rewarding process for multiplying plants! Hopefully we can dive into it more in future videos!
Part of the trick is to grow the plants sterile. When you grow your plants to cut tissue from use hydrogen peroxide to water them with, I use 10 ml a gallon of 35 percent hydrogen peroxide, I also clean the plants by spraying them with suppler, I also recommend zerotol. The plant must be frowns clean and as sterile as you can before the cuttings are taken. I’m not sure how aquatic plants would work.
Sulphur
Awesome video! Thank you. Can you say more about how you sterilize the plant before planting?
Use a fungicide and general antiseptic! I want to make a video diving deeper into this topic
@@prettyingreen And I want to order one of your Thai Cons! I was enjoying your website after posting my comment here.
This video was perfect timing! Thank you for this.
I bought a Thai constellation TC about a month ½ ago. I kept it with the lid on until it put out a new leaf which was touching the lid, so i removed it. She is in a high humidity area 70+%, and seems to be healthy but I'm not seeing roots on the bottom yet. Should I let her be or try to get a different dome on her? TIA.
Which media is she planted in? Make sure the cup has drainage holes-I’ve found it improves the quantity of rooting by having the extra air exchange at the root zone
@@prettyingreen 50%perlite 50% soil, and yes I have a drainage hole. TY.
Edit: I should clarify, I don't see roots in the bottom of the clear cup yet, the plant itself has roots. It had decent roots when I got it.
You will need to start moving her towards more lighting to encourage growth
@@Lee-nq6mh thank you!
Thanks for the sharing! Are you able to easily grow thousands of Thai con with tissue culture? What are some issues you’ve faced so far?
Damping off / infection has proven to be the most challenging issue. Costa Farms failed to grow thousands of these out, and they definitely know what they’re doing. The systemic root rot that comes with Thai con from tissue culture creates a massive hurdle also
Hello I have my Thai con marinating in its container and I see roots coming to the top, do I need to cover or just leave alone?
Whenever the roots grow up through the medium, I just cover with more mix over top of them 😄
Do you put holes in the parfait cup for drainage?
Not immediately-but later on, yes
Is there any way you can sterilize the perlite and stratum? Like baking it in the oven?
Yes! We are testing this exact method
Hi, should I soak perlite in a sterilising solution (Betadine/Hydrogen peroxide) as well as flush the plant? I will be receiving my first lot of 5 Thai con TC in about a month and really don’t want to kill them all. 😬
It would be best to do so. Just be careful with the strength of sterilization solution when applying it to plants / roots.
Can one node produce multiple tissue culture plants? Or do you even have to use a full node?
You can use a meristem ,
How long did it take from this smal thai con, to a big plant like in the back with big leafs, with your equipment?
That Large Thai Con is about 4 years old, but I would say it likely takes about 2 years to get to that size (in optimal conditions)
I appreciate your video. Question: Can you reuse status/perlite for acclimating other tissue cultures and if so, how would you sterilize the media? Thank you.
Yes you can! I actually bake it in the oven
@@prettyingreen would you mind sharing what temperature and for how long? Thanks so much. I am a new fan of your channel!
@@lorisoergel5286 Honestly I do like 30 mins at 350, but thats a kinda random amount. Make sure to mist it before you do so that the water droplets boil a bit! Please be careful baking it 😅
Do you think there's a way to increase your success rate? Have you tried activated charcoal in your mix? I don't really have any experience with this just trying to learn all I can before I start my own journey with a few hundred plants
Yes I think activated charcoal is a great addition. It’s all about the balance of cost, reuse-ability, and environmental impact for us. It’s a careful balance
@@prettyingreen do you think it would help with the rot
I root in rock wool plugs, the ones with the cut in them. This allows more air directly to the stem to develop calyxes and roots. I also mix my water I spray the plants with with 10 ml a gallon of hydrogen peroxide. This helps keep the sample sterile as possible and also helps the sample root.
The rock wool plugs are nuked in a microwave to sterilize them also for around 6 minutes.
hi i messaged you about a year ago coz i wanted to get your stuff for hydroponics and i was in canada, i decided not to go through with it that time but now i did! i got it like 10 days ago! shipping was good! and a lot of my plants im using it on are looking good so far! fingers crossed. thank you!!!!! and is there anyway you could pls share tips on how to acclimate plants imported from thailand? im planning to import some variegated raphidosphora tetrasperma from there. and maybe some monsteras. would really appreciate tips on how to acclimate them, im in canada by the way and we have super cold winters. thank you!!!!
Hey! Yes I remember!! So glad it actually arrived to Canada!!
Acclimating imports has to be one of the most challenging things in plant care. First you have to debug everything. Then, there’s always root rot. Treat with h202. Then need to prop and regrow roots. Then get into a safe rot free media, and attempt to grow leaves. 3-4 month process for the plant to look normal-ish.. but it can be done! Just long and tough to do
@@prettyingreen omg thanks!!!!!!!!!
7:24 great explanation of acclimatizing; my theory is that tissue culture luxury environment limits lignin / structural integrity…the “humidity hardening”, in my opinion, is shifting metabolism toward more lignin / production which you need to give time to happen. It is reason why plants can’t handle fast transition to atmospheric conditions.
It is sometimes call “hyperhydricity” or “glass”…it is where plant looks see through like a giant green glassy balloon that seems like it would melt instantly. The symptom is “apparent” sensitivity to water loss. I would suggest the plant does not have enough structural material so you need to kick 🦵 a little bit. Slow exposure to lower humidity is one way. I think future is in elicitors that you simply spray on to plants while still sterile (I have my own recipe). Happy to connect further on the technique. It is not universally used by plant tissue culture industry which costs them millions annually in yield losses, “acclimation time”, etc. Yes, an entire industry can be wrong.
Interesting for sure.
Are your lights on a rotator? I've thought about doing that too lol
They’re actually on a mover! I’ll show it In tomorrows video 😄
Did the stratum have to be rinsed off beforehand? Is it better to rinse it with distilled water before mixing it with the perlite?
I never rinse mine straight from the bag :)
Thank you!
@@prettyingreenjust wanted to give an update, my Thai con has survived the stratum and has grown 3 new leaves! Very healthy!
I want to see someone take the initial culture
What brand of fungicide do you use and where can I get it?
I forget the name of it, but there’s limited options for California. I would just check on Amazon or online for agricultural fungicide 😃
One of the best fungicides you can get is cheap sulphur ,
-like taking care of a brand new baby
-i lose 20-30%
🧐😪
legit though this is really helpful, i ordered my first tissue culture the other day (thai con, of course). i was just gonna put it in the fucking soil, so im a lot more prepared now.
thanks! (subscribed)
It's a super tough process with the Thai Constellation! But treat them like babies and you'll lose a smaller percentage. Thank you for the comment!
wow this seems like way too much work for something that can just easily die
The😢😅😢 analogy of the baby is great until you realize 30% of newborns die according to the analogy!😢😅😢
🥲😭😅
It is not like taking care of a brand new baby. Bad analogy. 😆
Hahah I guess it wasn’t as stressful as having my new born! But they definitely need specific biology introduction, warmth, humidity, and nutrition.