How to prepare MS media//Stock preparation with some tips and alternatives//Tissue culture media

#plant_biotechnology​ #plant_tissue_culture #Murashige_and_Skoogs_Medium​
Watch this video to understand how to prepare stock solutions and make MS medium for plant tissue culture techniques
Click to watch
--Cell suspension culture: • Cell Suspension Cultur... ​
--Embryo culture: • Plant EMBRYO Culture//... ​​
--Callus induction: • Callus induction proce... ​​​​​
--Callus induction from seed: • Callus induction from ... ​​​
--The stages of in-vitro seed germination: • Stages of In-vitro see... ​​​​​​
--To learn the process of in-vitro seed germination watch this video: • Invitro seed germinati... ​​​​​​
-- To learn basics of sterilization techniques watch this video: • Types of Sterilization... ​​​​​​
-- To learn all about the Tissue culture lab, the instruments used watch this video: • Plant Tissue culture L... ​​​​​​
-- Learn how to fumigate the lab by watching this video : • Fumigation of Lab ​​​​​​
-- Visual tour of microbiology lab: • Microbiology lab and t... ​​​​​​
-- Visual tour of molecular biology lab: • Molecular biology lab ​​​​​​
-- Visual tour of a general life science laboratory: • Life science research ... ​​​​

[@ashusingh6813]

You efforts show... How much you are interested in imparting knowledge. Good work

[@strange9673]

We appreciate you brother thank you for sharing your knowledge of the plant tissue culture techniques. Much love from Oaxaca, Mexico🤟🏽🍄🐍 -Dr. Strange

[@Dr.Rakesh.B]

Thank you for the support 🙏

[@joyfull244]

Hi
I am also working on bacopa monnieri plants tissue culture and also cell suspension culture coincidence I see your video today from Karnataka ❤️

[@Dr.Rakesh.B]

Great to hear, wish you all the best 👏

[@nxtlvlyt4466]

I love your videos 🙌😎 very informative and inspirational. One day I hope to work in a lab such as yours😄.

[@Dr.Rakesh.B]

Means a lot 🙏

[@makemoney4386]

Excellent video,sound,tropics And important is your effort..
Lots of thanks for your clear understandable communication.

[@Dr.Rakesh.B]

Welcome:)

[@mudavathpadma4976]

Thank you so much for ur effort sir ur realy genious sir👌...u considered our request and updated video we r very thnk ful to you:)

[@prabhdeepkaur7777]

Great video sir 👍👍👍

[@sanathekanayake503]

Yes ,thank you very much . good lesson as bigginers like me for tissue culture

[@Dr.Rakesh.B]

:)

[@ankitachowdhury5268]

Thank you so much for your effort sir.......your all videos are very beneficial and informative ❤️

[@ExplosiveFists]

so glad i came across these videos

[@prabhdeepkaur7777]

Learnt many new things due to this video ✨✨ thanks a lot

[@Dr.Rakesh.B]

Im happy it was useful:) This makes my effort and hardships valuable 🙏

[@abiabi-hx6vx]

Very usefull sir

[@reformerjugnetech7370]

very informative

[@SantelWood]

Can you suggest the agencies supplying the equipments and the spice required for installing a most modern well equipped lab for Research purpose.

[@gethsamanegardens909]

Very informative session brother...thank you

[@amtd3808]

Sir please make video on preparation of Harmone stock solution for PTC..

[@keerthic9692]

Great explanation.Thank you Sir.

[@Dr.Rakesh.B]

:)

[@creatodayin4924]

Thank you sir really informative video 👌❤

[@AliHaider-fq8mx]

Great video

[@Dr.Rakesh.B]

Thank you :)

[@foxhixwebsite62]

मोरल ऑफ द स्टोरी, आप भाई ये बताओ कि इन सभी केमिकल के ऐसे नाम जो सरल हो और ये केमिकल मिलता कहां है।

[@anushkagupta4271]

Thankyou sir.

[@SantelWood]

I want to follow your class

[@shravanigali4739]

If we get individual chemicals of ammonia nitrate and potassium nitrate, should we take both of 35.5 gm separately

[@richisika4989]

Nice vedio.Please also make a video on estimation of beta-corotene

[@darshanav5072]

Really informative vedio...plz give me the details about ready made MS media power and which is the good brand?

[@Dr.Rakesh.B]

Himedia. I brought it through online.

[@SantelWood]

Since you told me that your lab is for post graduate students.

[@vawcreations5887]

I REALLY LIKE HOW YOU EXPLAIN.
GREAT WORK.
PLS MENTION THE LINK TO THE CALCULAION VIDEOS.
THANKS AGAIN.

[@Dr.Rakesh.B]

Thank you
czcams.com/video/co4U3vJmFP4/video.html

[@ZK292]

@@Dr.Rakesh.B thank u sooo much sir...Very helpful

[@neelamsingh8882]

Sir plz add the link from where u brought that, Supplement for Ms media.?? I need alternative for Nh4NO3

[@Dr.Rakesh.B]

Buy from Himedia, browse in internet

[@neelamsingh8882]

Yes I had searched on HIMEDIA but Sir I can't find any product like this.. Plz sir can u tell the cas number?? Which is written on that bottle..??

[@neelamsingh8882]

Sir plzz 🙏 reply.. I want to know 😓 from where u brought this supplement for Ms medium..!! Because I have searched alot in Himedia.. Even I got ur cas no.. But then thus.. They are saying we don't have any products like this.. So plz 🙏help me sir.. Plz snd me the link or anything from where u brought this product..????

[@Dr.Rakesh.B]

@@neelamsingh8882 I didn't buy, my lab had it already. And now I'm not in my lab anymore.

[@neelamsingh8882]

Then Sir plz.. Tell me any alternative chemicals name which I can use instead of ammonium nitrate..???

[@bianconiglio1235]

❤

[@mayankrawat2934]

Any substitute for ammonium chloride, tell if any ??

[@Dr.Rakesh.B]

There's no equivalent substitute for these compounds of MS media but you can alter for eg, u can take some other chloride sources like NaCl and ammonium sources like ammonium nitrate and check.

[@poornimajoshi5537]

chart is not clear

[@TheOrganicSpade]

Hi, I want to learn TC. Ive managed to find most ingredients except for PPM in india. Could you tell me where can one buy plant tissue culture ingredients in india?

[@Dr.Rakesh.B]

We order online from Himedia

[@TheOrganicSpade]

Oh ok...but I'm sure they only sell to labs. Do you know of any suppliers who will sell to individuals?

[@Dr.Rakesh.B]

No idea

[@greenhut1642]

What light is used, which is the container used. From where can I buy. Is it possible to at home

[@restrictedendonuclease4340]

Hello sir I just did my 12th and I'd always had intrested in recombinant organism..so as I was getting bored I took leaves of citrus lemon and psidium guajava and isolated their protoplast ...and I'd mixed those protoplast in a tube containing polyethylene glycol (peg) for protoplast fusion ...now in a day I see that there is something green brown colur mass or coagulate is formed ...can you tell me what it is or if it's a hybrid protoplast how to culture it

[@Dr.Rakesh.B]

Im sorry,.i haven't done protoplast culture . I do not have practical exposure. Nevertheless u can read articles to know their culture conditions.

[@restrictedendonuclease4340]

@@Dr.Rakesh.B see I'm doing some incredible mutation in animal cell as well as plant ... I'm extracting gene which are responsible for cellular totipotency in plant cell

[@restrictedendonuclease4340]

@@Dr.Rakesh.B and I don't know much things practicality ... I would love if you could tell me something

[@restrictedendonuclease4340]

@@Dr.Rakesh.B how to extract gene from a plant cell like from agrobacterium tumificences or such kinda vectors

[@ranajitghorui6376]

Sir all product Himedia?

[@Dr.Rakesh.B]

Yes. Our lab has Himedia but u can use other companies too

[@kishorregmi2265]

sir could you send me the chart of ms media shown in your video

[@Dr.Rakesh.B]

I do not have that chart dear. You can take it from Google.

[@shravanigali4739]

Plzz reply

[@jamesjoseph5662]

Hlo sir I want to contact u

[@Dr.Rakesh.B]

Mail me at [e-mail]