Thank you so much Francisco, I love plants they are my favourite hobby and the best things for my mental health. tissue culture growing is all I want to do, I aspire to make a living like you here in north of England where it's dark and gloomy for most of the year. The knowledge is priceless to me and I really appreciate you sharing your knowledge ❤ I'm most interested in self heading philodendrons because they are beautiful and I have far too many haha
Thank you so much for all of your videos!! You are amazing and I am so excited to do a consultation call once I get my lab set up. One question on the “half strength MS” in the recipe- do you have a video where you explain what half strength would be? Does it have to be ordered that way or is it a dilution ratio? Thank you so much!
thank you so much for your wonderful feedback. We will be delighted to have a chat with you about tissue culture and your lab setup. And, half-strength media is half the concentration you use in 1 liter while preparing full strength. So, for example, if you use 4.4 grams MS media/ liter, you will need 2.2 grams MS media/ liter for half strength.
Can you guide me with a protocol for ginger (for surface sterilization) please? And also having trouble of how to isolate the meristem from rhizome sprouts?
Meristem isolation is something that we cover in our master class. So, if you're interested you can join us in the next one: www.plantcelltechnology.com/master-classes/ About ginger, we didn't try it in our lab. But you can easily find many protocols online in research papers.
Hi, We haven't worked with blueberry plants in our lab yet, so we don't have a developed protocol for the same. However, you can try searching the internet, you will find many research papers mentioning the protocol that you can use.
Thanks Francisco for another great video. My plant is the family Costaceae which I had been unable to find in the literature until I tried Google Scholar and found a couple of papers which I hope to get from a botanist friend in India. I have also found some good information on the sister family Zingiberaceae but not sure it would be applicable. What I would like to know is whether or not there is a source with GENERAL information about the different protocols for different types of explant material - differentiated cells vs. non-differentiated cells. Seeds, leaves, meristems, etc., are there general requirements like starting in the dark vs. the light, using auxins vs. cytokinins, or both and in what general ratio? That would at least give me a starting point if I want to experiment with leaves, for example. Are seeds normally started at lower strength MS? - that kind of question. Do you know a good reference source to get that generalized information?
I’m not sure if there is a website like that. I think most people apply their own knowledge and experience working with similar plants to determine what would be a good starting point.
Currently, we are working on houseplants and carnivorous plants in our lab. But, if we get ourr hands on the plant. we'll definitely give it a try. Thanks!
Thank you for this video. Can you tell me if anyone is allowed to use protocols on plants that they purchase from stores if these plants have a license or patent. I don’t know if I am using the correct terminology
@@PlantCellTechnology You cannot "tissue culture" any plant you want. If it is patented or has some other intellectual property protection, it is prohibited to reproduce the plant asexually. Doesn't matter whether you're going to sell it or sell it but not name what it is. The act of asexual propagation is forbidden.
@@dikdebruijn5101 Patent is another topic. What we're saying is that in general any plant can be tissue cultured. So, if you can culture it or not, you'll have to check on your own. But theoretically, tissue culture can be used to grow any plant, provided an effective protocol. We're talking about the technique, not any specific plant.
Benthamiana! It remains me when I used to work at the university's lab 😄. It grows very fast from seed in TC. I think I could have plants big enough for transformations in 4-6 weeks.
If you are getting started, first look if you have ample space and a budget of at least $300-$500 to get started with the technique. Decide why you want to do tissue culture, what plant do you want to grow, and the materials you need. You can reach out to us at info@plantcelltechnology.com and our team will assist you with all your tissue culture requiremnets. Hope this helps!
I said the Goodyera, and I deliberately did not pick a species to not limit it and give more chance, the reason I told this one is because I try to search for it every few weeks and there are papers related to seed germination, which is useless because Goodyera seeeds is pretty much impossible to come by, pollinating them is also not an option. There is none that works with anything other than seeds and even most of those are related to european temperate species. The video is not over but I'll be super suprised if you found one.
Actually for the second time you drigted away and ended up with Cymbidium related papers, which is quite different. You actually find something I did not so far, but even though I copied the exact title to google scholar it's not listed for me, I don't if it could be some regional limitation.
@@norbertpometko Thanks so much Norberto. You can try different names of the plant, common names or scientific name,s or any regional name you know. sometimes those work.
You can find colleges that provide tissue culture education. Some offer it mix with other subjects whereas some provide it as a certification course. But, the first step is to list out such colleges and apply for the course.
We aren't working on potatoes currently in our lab. But, using the method we told in this video you can search for a protocol for potato tissue culture.
Yeah, right! Sometimes even when you are working with the same species, the same protocol "might" not work for you, because the plants are grown in a different environment, and handled in a different way.
In 1983 I was propagating orchids in tissue culture. We just blended banana, potato and agar with a few hormones. Great for all cymbidiums.
Amazing! It's really great to know how you have expanded to different plants as well.
Just found you're page and so thankful!
You're the best man, thank you.
Thank you so much for watching our videos.
Que bueno que tienes éstos momentos tan bello como das tus comentarios y millones de bendiciones
Thank you so much Francisco, I love plants they are my favourite hobby and the best things for my mental health. tissue culture growing is all I want to do, I aspire to make a living like you here in north of England where it's dark and gloomy for most of the year. The knowledge is priceless to me and I really appreciate you sharing your knowledge ❤ I'm most interested in self heading philodendrons because they are beautiful and I have far too many haha
I was trying to find the protocol for fancy Gloxinia. …. I thought it interesting that the protocol you found said the AGE OF LEAF was very important
Yes. it does matter.
Thank you so much for all of your videos!! You are amazing and I am so excited to do a consultation call once I get my lab set up. One question on the “half strength MS” in the recipe- do you have a video where you explain what half strength would be? Does it have to be ordered that way or is it a dilution ratio? Thank you so much!
thank you so much for your wonderful feedback. We will be delighted to have a chat with you about tissue culture and your lab setup.
And, half-strength media is half the concentration you use in 1 liter while preparing full strength. So, for example, if you use 4.4 grams MS media/ liter, you will need 2.2 grams MS media/ liter for half strength.
Can you guide me with a protocol for ginger (for surface sterilization) please? And also having trouble of how to isolate the meristem from rhizome sprouts?
Meristem isolation is something that we cover in our master class. So, if you're interested you can join us in the next one: www.plantcelltechnology.com/master-classes/
About ginger, we didn't try it in our lab. But you can easily find many protocols online in research papers.
Hello sir.
I needed blue berry plant tissue culture protocol and Ms midiya proportion.
Hi,
We haven't worked with blueberry plants in our lab yet, so we don't have a developed protocol for the same. However, you can try searching the internet, you will find many research papers mentioning the protocol that you can use.
39 minutes !!! WTF 😂 Thx a lot
PS : I love your new hair cut 😜
haha! Thanks!
Thanks Francisco for another great video. My plant is the family Costaceae which I had been unable to find in the literature until I tried Google Scholar and found a couple of papers which I hope to get from a botanist friend in India. I have also found some good information on the sister family Zingiberaceae but not sure it would be applicable. What I would like to know is whether or not there is a source with GENERAL information about the different protocols for different types of explant material - differentiated cells vs. non-differentiated cells. Seeds, leaves, meristems, etc., are there general requirements like starting in the dark vs. the light, using auxins vs. cytokinins, or both and in what general ratio? That would at least give me a starting point if I want to experiment with leaves, for example. Are seeds normally started at lower strength MS? - that kind of question. Do you know a good reference source to get that generalized information?
I’m not sure if there is a website like that. I think most people apply their own knowledge and experience working with similar plants to determine what would be a good starting point.
boss u r great
please make another content for astarica potato tissue culture
full system and many more details
Currently, we are working on houseplants and carnivorous plants in our lab. But, if we get ourr hands on the plant. we'll definitely give it a try.
Thanks!
thank u for your think
Hi Fancisco, great seeing your vedios, can you suggest any protocols for propagating Pear and apples? your guidance is appreciated
By using the approach we have taught in the video, you can find protocols of any plant if available over the internet, including apple and pear.
Evaluate the potential of tissues culture on endangered plant apeciss
There are multiple papers on it available on the internet. Are you working on endangered species?
Good day, Sir! May I ask how many months it take for cacao and coffee? Tissue culture
It depends on the plant species. Generally, a plant takes 3-6 weeks to initiate.
Thank you for posting :)
Thanks!
Thank you for this video. Can you tell me if anyone is allowed to use protocols on plants that they purchase from stores if these plants have a license or patent. I don’t know if I am using the correct terminology
Hi! Couldn't get your question. But, you can tissue culture any plant you want.
@@PlantCellTechnology You cannot "tissue culture" any plant you want. If it is patented or has some other intellectual property protection, it is prohibited to reproduce the plant asexually. Doesn't matter whether you're going to sell it or sell it but not name what it is. The act of asexual propagation is forbidden.
@@dikdebruijn5101 Patent is another topic. What we're saying is that in general any plant can be tissue cultured. So, if you can culture it or not, you'll have to check on your own. But theoretically, tissue culture can be used to grow any plant, provided an effective protocol. We're talking about the technique, not any specific plant.
what about the cold plasma treatment
What do you want to know about them?
Chat gpt work ?in this field
Can u tell me how much time the tissue culture of nicotiana Benthamiana take ?
Benthamiana! It remains me when I used to work at the university's lab 😄. It grows very fast from seed in TC. I think I could have plants big enough for transformations in 4-6 weeks.
Where would you get just a basic laminar flow hood? Im looking to get one just am not sure really where to start
If you are getting started, first look if you have ample space and a budget of at least $300-$500 to get started with the technique. Decide why you want to do tissue culture, what plant do you want to grow, and the materials you need. You can reach out to us at info@plantcelltechnology.com and our team will assist you with all your tissue culture requiremnets.
Hope this helps!
@PlantCellTechnology thank you for the advise. Appreciate it. I'm just vegetable gardening
@@JordonCope, Of course! Happy to help!
@@JordonCope, of course! Happy to help!
I said the Goodyera, and I deliberately did not pick a species to not limit it and give more chance, the reason I told this one is because I try to search for it every few weeks and there are papers related to seed germination, which is useless because Goodyera seeeds is pretty much impossible to come by, pollinating them is also not an option. There is none that works with anything other than seeds and even most of those are related to european temperate species. The video is not over but I'll be super suprised if you found one.
Actually for the second time you drigted away and ended up with Cymbidium related papers, which is quite different. You actually find something I did not so far, but even though I copied the exact title to google scholar it's not listed for me, I don't if it could be some regional limitation.
Otherwise I think it was useful information and good video.
@@norbertpometko Thanks so much Norberto. You can try different names of the plant, common names or scientific name,s or any regional name you know. sometimes those work.
@@norbertpometko pro tip, use a VPN service so you can access the WHOLE internet.
Sir What is the media for cymbidium orchid?
Hi,
We don't work on the plan, so not aware of the media recipe. However, you can search it online. You will find many papers around the plant.
Francisco, how do i get a college degree in plant tissue culture?
You can find colleges that provide tissue culture education. Some offer it mix with other subjects whereas some provide it as a certification course. But, the first step is to list out such colleges and apply for the course.
Are they protocols for fruit trees too?
Yes, there are. You can find plenty in google search.
May I ask your protocol for nepenthes seeds? Thanks
By using the approach we have taught in the video, you can find protocols of any plant if available over the internet. Thanks!
Can we use water agar for tissue culture? If we can use agar water then how much amount of agar agar is used in water??
Do you mean liquid media? Can you explain what you mean by water agar?
@@PlantCellTechnology water agar mean only agar and water
@@momnaaesthetic No. You need any nutrient media, like MS media, to grow your plants in lab conditions.
@@PlantCellTechnology ok thanku
Please tell me the best media for potato tissue culture, tell me the exact amount that makes up to a litre please
We aren't working on potatoes currently in our lab. But, using the method we told in this video you can search for a protocol for potato tissue culture.
we do have some blogs that you might find useful for you: www.plantcelltechnology.com/search.php?search_query=potato§ion=content
@@PlantCellTechnology can you please send me the distributor's physical address in South Africa
I finally got the media,MS, Agar microwave,pH reader and water based fertilizers suitable for my future plants
what is the recipe for canna?
Even after he made a whole video on how to find protocols, you still want someone else to do the work for you.
@@Origmist that's 2023 for you 😜
@@jonesrdh7170Too-Funny but absolutely on point and hilarious how lazy people can be nowadays. 2023! Woot Woot
21:20 so what you're saying is every protocol regarding tissue culture are not compatible with the same species?
Yeah, right! Sometimes even when you are working with the same species, the same protocol "might" not work for you, because the plants are grown in a different environment, and handled in a different way.