How to Make Tissue Culture Media (Demo)
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- čas přidán 3. 02. 2023
- Happy to answer any questions in the comments! Making tissue culture media is the easiest part of the micropropagation process because it doesn't need to be done in sterile conditions, since we autoclave it. Be careful with the pressure cooker. In the future, I do plan to make a video specifically about using a pressure cooker as an autoclave.
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awesome introduction vid, just learned this was a thing. brings me back to chem lab freshman year of college XD
Thank you so much this was really informative ‼️
Awesome video! Do you have plans to create protocols to follow along with these videos?
This helps me alot, thank you
Thanks the clip is very informative. Got two questions: 1)do Auxins break down under high temperature and 2) at what temperature do you normally set the magnetic stirrer? Massive thanks in advance
Awesome, many thanks
Great video! Do plants grow in those little plastic containers after a while?
Can I use jam or mayonnaise bottles with solid caps
How long it takes from zero to 100 percent? I mean to do through all stages of TC to the point of acclimation? Like 30 weeks?
Cool video!
Which plants are feasible for these protocol?
Can I use hoosier hills farms agar for tc
Can you plz help me the hormones needed and quantity for callus formation
where could i find the protocols for cannabis? thanks.
Plant hormones are heat labile , wont it degrade during autoclaving?
Great videos! Is there a recommended temperature for storing the jars of media with cuttings?
The protocol that you follow will specify the optimal temperature! I don't have the AC in my home lab zoned separately from my home, so these plants stay around 72 - 75 degrees, but some protocols will call for a slightly higher or lower temp.
Я правильно понял, что крышки на банки в автоклаве просто накладываются, не закручиваются, а закручиваются только после автоклавирования?
Can you make a video or comment a recipe for cannabis.
She just showed you
Please can you list the ingredients
Hello Laur, Thank you for the quality content! I’m hooked! I’m from Brazil, there is zero information in Portuguese abou the subject. How can I get a pdf of the book you mentioned?
When you google "plants from test tubes pdf" the first result should be the 3rd edition of the book as pdf.
Very helpful! You just earn a sub. I have questions. For tc jar, is it necessary to have small ventilation hole? Ive seen some use air tight glass jar. Which one better? Do tc plants even need ventilation?
For the multiplication and rooting stages, you want the jar to be completely sealed with no ventilation hole to maintain 100% humidity. To prepare the plants for the final stage of tissue culture, acclimatization, you may change the lid to a vented lid, to gradually acclimate the plantlets to lower humidity before you take them out of tissue culture and plant them in soil.
@@plantsinjars got it thanks!
Has anyone found the protocols for cannabis?
How do you find out what PGR you need for specific plants?
I use research gate and google scholar to find protocols
Can you suggest the full set ingredient for making the medium for cannabis?
Do the plastic pipette attachment things just go in a normal trash can?
Yes. I have a special container for disposing scalpel blades but the pipettes get thrown in the normal trash
Great
Bravo, I like your educational videos. Please make a video about cloning bamboo and burma teakwood.
Thank you for this! Silly question: None of the components added to the media are degraded by the autoclave process?
Some plant growth regulators are slightly degraded when autoclaved, but it is ok. Antibiotics on the other hand, should be filter sterilized and added after autoclaving.
Thank you!@@JesusGonzalez-gr9wz
Wish the ingredients were cheap and easily available as well ;)
So you are using 1:1 auxin and cytokinen? Does it has particular goal with the ratio? Thankyou.
I was just following a stage I african violet protocol that I found in a book. Different plants / stages of tissue culture will have different ratios though
@@plantsinjarswow thank you for your rapid reply😆 i mean auxin and cytokinen affect each other right? So if it's in the same ratio what is the aim, is it to grow the root or shoot?
Could you help me find a media for monstera / philodendron please 🙏
I share the recipe in my most recent vid :)
What is the brand and model of your PH meter?
And how often does it need calibration?
Do you store it in KCl solution?
Are u satisfied with its accuracy?
It's called VIVOSUN 3-in-1 Digital pH Meter with ATC, ±0.1 pH Accuracy on Amazon. I calibrate it every time I use it. This one doesn't get stored in kcl. I will probably replace it within the year though, they are known to become inaccurate after awhile.
@@plantsinjars do u recommend vivosun ph meter for beginners like me? I'm so confused about which brand I should buy......😵💫
Ps: I can't spend more than $50.
@@mahyarshokraeian I would just get a cheap one like this: www.amazon.com/VIVOSUN-0-05ph-Accuracy-Readout-Temperature/dp/B06XKMH86J/?_encoding=UTF8&pd_rd_w=93qkp&content-id=amzn1.sym.bc5f3394-3b4c-4031-8ac0-18107ac75816&pf_rd_p=bc5f3394-3b4c-4031-8ac0-18107ac75816&pf_rd_r=4E96BEPDMG0F0Z0KMR1B&pd_rd_wg=si6PP&pd_rd_r=cfff3a88-e779-4f4f-9dbd-3328e0c64201&ref_=pd_gw_ci_mcx_mr_hp_atf_m
Does anyone know if they are available in Germany? Under which name? Couldn’t find thisstuff….
There are some links to EU suppliers on my discord server! Link to join is in the description of my more recent vids
So wonderful!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! =)
❤❤❤❤
Hi
Shouldn't you wear gloves when handling these hormones ?!
Usually… yes. But I’m pretty sure she uses kinetin as her cytokinin of choice. While it’s not 10000% harmless it’s naturally derived and a lot less harmful than say BAP etc. I personally don’t even want to be in the same house as BAP.
@@andrewdarland8600Why? BAP isn’t radioactive. It’s not some deadly poison. It’s no worse than the other common PGRs.
No, because she is a strong independent woman.
@andrewdarland8600 is kinetin same thing as bap? For rooting