Preparation of MS Media for plant tissue culture (Demonstration video)

Composition of MS media file: imgur.com/I32r1F9
Note: The media after autoclaving looks yellow in this video. For demonstration purposes, we used a different media afterwards to save time, however, please keep in mind that MS media does not change color like this while you do this experiment in real.

[@harshbhosale4344]

What is stock A %

[@pushpalatha9867]

Excellent work

[@shantiagrotech]

very good video for Beginners. Medam where can I purchase the MS MEDIA.

[@prathapagiriharish2687]

Nice explanation man

[@jesussaves3904]

clear explananation thank u

[@kishanmanch1094]

Please kaha melega

[@x1Conan3Spartan0x]

I was confused on the recipe until I seen this video. Does the Pre-made MS powder have rooting hormone?

[@steamerpowered]

no, you add whatever required hormones for what your are growing.

[@mohammadasadullah5171]

If I dissolve all the materials into water and adjust ph than also add agar. But I have no time to autoclave. how many days can keep the media in 4 degree? Or I need to prepare again

[@Floral39]

It should be fine at 4C for 1 or 2 days

[@meetasrivastava3852]

What is the amount of sucrose to be added if it is absent in MS powder ?

[@babusona3217]

30 gm

[@avisiktachakraborty3438]

Chemical biological products like waterpowder

[@neilsontamang3251]

What did u add to lower the pH

[@babusona3217]

Naoh

[@burjisbhat8633]

Autoclave temp for media?

[@babusona3217]

15 psi pressure

[@surenderchilwal1018]

what is the composition of stock B,C,D

[@biomedgp]

I have attached a file in the description of this video. You can find the detailed composition in that. Please do subscribe to my channel. Thank you.

[@yashikanalla299]

Can you please make a video on how to prepare mineral salts media liquid and how to prepare stock of micronutrients, also how yo use ph meter acid base and calibration..
Thank you

[@biomedgp]

Sure :)

[@yashikanalla299]

Thanks ❤

[@sambitbanerjee7843]

How to measure ph

[@biomedgp]

Using a pH meter.

[@seethamahalakshmi6651]

😊

[@urwaali3696]

Did you add Agar at the end when it solidifies? Please reply!

[@frienson_]

The media does not solidify until agar is added as it is the solidifying agent

[@babusona3217]

Or use gellan gum

[@prathapagiriharish2687]

Mam

[@izzapz]

Why MS medium is brown?

[@biomedgp]

Hello. I have mentioned this in the description. The media shown is some other media. This was only a demonstration.

[@danieljheelan5256]

It depends on the agar you are using. Some looks more brown.