Multiplex PCR
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- čas přidán 15. 07. 2024
- Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target. This technique requires two or more probes that can be distinguished from each other and detected simultaneously.
Thank you so much for the explanation.It clarified my confusions.
The best explanation, thank you so much!!!
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Great explanaation! Thank you for your time.
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Great video
To the point
Thnk u so much sir
The video is very informative and easy to understand. Thank you
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Is it that although the amplicon size must not differ too much it must still be different enough so that distinct bands may be observed during gel electrophoresis?
It is very clear explanation about multiplex, thanks
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Feels like this video could be sponsored by SpeeDx - same fundamental but totally different molecular biology
Great video!
2:47 If multiplex PCR requires that the template size does not vary too much, how would it be detected separately on the agarose gel as they would be having similar molecular weights?
Very Informative, I love it
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thanks for the explanation
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Thank you ❤️
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Super vid. Thanks.
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Best Sir. Easily you Explained... Thanks alot
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@@animatedbiologywitharpan Yes sir
Sir plzzz upload videos of primer designing with full details.
How can we identify rs no,
Second for RFLP and ARMS primer
designing videos also
thanks a lot sir.
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You are really best
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Thank you for the clear explanation.
Please share this in your college group
hello, I do not understand English very well I speak French but I found your video great however there is one thing that I would like to know in the tube we put the patient's DNA only then the primers of different viruses and we look at gel electrophoresis whether it's to amplify or not. my question is at no time we add the DNA or the viral DNA viruses can you answer me please even in English I will understand thank you
Il est nécessaire d'ajouter l'ADN du virus dans un autre tube et faire le même PCR pour le positive contrôle
Viral DNA is already present in the patient's sample. No need to add it separately.
Sir, please make a video on allele specific PCR technique...
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Good one just like @sciencepoint
So for covid we can use multiplex PCR??
Yes multiplexing can be done
Always best
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Awesome 3 minutes . Thanks a lot.
each of my samples contains one bacteria and one virus. Can I use multiplex pcr to quantify them in each sample and compare those ?
@@dr.urmibhattacharjee6919 yes you can, if the pcr product size is different lets say 400bp for bacteria specific gene and 600bp for virus specific gene then its possible .
@@dr.urmibhattacharjee6919 Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Sir, can we use multiplex PCR for early cancer detection??
Yes theoretically it can be used
Please make a video on dna fingerprinting
Thanks for your suggestion will make one. But i do have vodeo on southern bloting and RFLP analysis
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@@animatedbiologywitharpan I am following you on both
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