Inverse PCR

Inverse polymerase chain reaction (Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence.

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Wonderful teaching style

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Thank you

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[@anshikarajput2864]

Why we call this as inverse pcr??

[@priyathakur423]

Why there is smear in inverse PCR gel run?

[@a.n.5630]

How should you design the primers in this case?

[@animatedbiologywitharpan]

please refer to this one support.snapgene.com/hc/en-us/articles/10384197784084-Inverse-PCR

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Nicely explained 🌼🌼

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[@soumyaartz8912]

Why cann't we simply sequence it after restriction digestion?....why to amplify it by PCR?

[@animatedbiologywitharpan]

For sequencing as well you need good amount of starting material. Some times it's a technical issue with amount of sample

[@kamariweaver1536]

Can you do a video on western blotting?

[@animatedbiologywitharpan]

czcams.com/video/Yh69yHJMWPc/video.html check the I button for links to 2d PAGE and sds PAGE

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@@animatedbiologywitharpan Got it, thanks!

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[@suchikar7901]

When sequence is already unknown how to know which restriction enzyme required when do restriction digested?

[@user-kn7hc8xo8c]

You should choose the endonuclease restriction that doesn't cut your transposone or known DNA region

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3:04

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