Heyy, I did LAMP today. The experimental setup was pretty straightforward. BUT, trying to understand the principles behind it, as concisely explained in your video is quite a challenge for me to wrap my head around it. I'll have to do more reading for sure.. 😂 Thanks for the explanation!
In PCR there are 3 cycles which combined take up some time to generate the daughter strands. In LAMP the synthesis continues constantly.. further: using 6 primers in case of LAMP, provides more contacts for polymerase recruitment!
The animation is very helpful. It will be even better if was shown at least one cycle after the annealing of external primers, indicated in 3:14. Unfortunately, I live in Brazil and my financial situation is not so good as I would like, but your animations deserve much more than $ 2.00. And thank you for answering my questions!
@@mariociencia12 Why do you donate, when your financial situation is not so good? I am extremely thankful, but I have to make that clear: I would not want anyone to donate if they have to keep an eye on their money!! Thanks anyways :) *And you´re right - I spared myself the animation of the cycle
@@henrikslab I would like to donate more! You deserve! But the donation that I did will do not compromise me. Do not worry. I also did it as an example, hoping that other viewers do the same
I read that most DNA denatured at 75 degrees, does the DNA denatured at 60-65 degrees in LAMP or just some location? Can this become a limitation of LAMP?
Thanks for the work that you put in. Hit me a massage if you like. I am a master in biochemistry and molecular biology maybe we can arrange something together.
This is the clearest explanation I've found. THANK YOU!!
This was giving me a migraine and now i only have a small headache. Thank you for the help!
Same here.. it was the reason why I did the video :D
Although it seems simple, it's difficult to understand.. You made it really easy. Thank you for the explanation. :)
How is each amplicon produced? How is the strand displacement accomplished, is it a function of the polymerase? Why is the loop formation necessary?
the most clear explain ..
thanks
How both DNA denaturation and primer binding is occuring at same temperature?As both of them are opposite reaction?
Heyy, I did LAMP today. The experimental setup was pretty straightforward. BUT, trying to understand the principles behind it, as concisely explained in your video is quite a challenge for me to wrap my head around it.
I'll have to do more reading for sure.. 😂
Thanks for the explanation!
Hello, I need your help.... ahaha
Can u explain me plz
Thank you for your video. i praise you to talk slowly and good pronunciation!! i'm about to subscribe your channel.
Loved this explaination!!!
Very good!!!! Thank you!
Excellent! Finally, I understood the loop structure formation. However, how does it makes the DNA duplication faster?
In PCR there are 3 cycles which combined take up some time to generate the daughter strands. In LAMP the synthesis continues constantly.. further: using 6 primers in case of LAMP, provides more contacts for polymerase recruitment!
perfect , thank you
Hi there. Is there any way you can turn on captions for this video? Thank you
Like and subscribed. Very good job 👏👍
Valeu!
The animation is very helpful. It will be even better if was shown at least one cycle after the annealing of external primers, indicated in 3:14. Unfortunately, I live in Brazil and my financial situation is not so good as I would like, but your animations deserve much more than $ 2.00. And thank you for answering my questions!
@@mariociencia12 Why do you donate, when your financial situation is not so good? I am extremely thankful, but I have to make that clear: I would not want anyone to donate if they have to keep an eye on their money!! Thanks anyways :)
*And you´re right - I spared myself the animation of the cycle
@@henrikslab I would like to donate more! You deserve! But the donation that I did will do not compromise me. Do not worry. I also did it as an example, hoping that other viewers do the same
@@mariociencia12 That´s very kind of you! But it helps me also so much when people like, subscribe and share the channel with friends!
perfect!
great👍👍👍
I read that most DNA denatured at 75 degrees, does the DNA denatured at 60-65 degrees in LAMP or just some location? Can this become a limitation of LAMP?
Why do you only have the first product after the steps at 2:50. I thought the very first product is the one at 2:00 ?
I don't know how the first strand invasion occurs
capooooooooooooooooooooo
yey
Thanks for the work that you put in. Hit me a massage if you like. I am a master in biochemistry and molecular biology maybe we can arrange something together.