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Love your channel and the way you make difficult concepts easy. I had a question about primer property checking using Primer-BLAST. I have a primer that contains degeneracy/wobbles and I want to check its properties such as Tm, self-complementarity, self 3’ complementary using the primer- blast… However, when I use the primer Blast and search, it states that “ambiguity letters other than N are not allowed in the primer sequence” So that forced me to replace all the degenerate/wobble letters with N. Would that still make the property results correct? Am I still getting the right answers if they are replaced with N? Thank you so much, I would really appreciate some insight on this, as I can not find an answer anywhere.
Clear explanation. But do add some more text in your slides, like principle of the technique & lable each & every component in the slides. So that it will be much useful to us. Thank you.
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Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
If I get a annealing temperature 66.4 and got single peak of melting curve, can I use that annealing temperature? While the Melting curve temperature range is 65_95’c ….what you suggest?
No no it can bind to any double stranded dna, but the pcr will selectively amplify the target gene so in the reaction tube there will be huge quantity of target with which sybr green can bind
The baseline of the real-time PCR reaction refers to the signal level during the initial cycles of PCR, usually cycles 3 to 15, in which there is little change in fluorescent signal. The low-level signal of the baseline can be equated to the background or the “noise” of the reaction
I am not sure that if he is an ( whatsoever you have mentioned ). I know him personally, he had to do a lot of struggle in his life . Thanks for your compliments though.
Good explanations.......what if the ct value of control and treated sample are same say 26 for both. Does it mean that the transcript level is same in both.
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sir abhi window period mai hu sir actually next month mai canada jaaa rha hu pr m glti se hepatitis C ke contact mai agya hu … sir confirm nhi horha….. pls btao sir kuch 25 febuary ko needle use ki thi hep c postive ldke ki sir fr 10 din baad hcv pcr rna quantitative test krwaya …. Usme not detected aya … sir plss btao next test kab karwau ki accurate aajaye jldi mne canada jana h.. pls help sir🙏🙏🙏
Sir i recently done test rna pcr the sample was collected in heparin tube will this affect my result? Or the person carrying this test how will he know about inhibitors ?it was qualitative test.
This is that guy on youtube that explains ambiguous concepts very well, which you are searching for. Amazing!
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Really well done, thanks! I wish someone had walked me through qPCR this well when I first started running them!
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I gave different playlists on different techniques , so don't forget to check out all of that
Using this to prep for a mock interview tomorrow. You're a lifesaver. Thank you!
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very good overview. can you please make a detailed one on melt curve analysis and primer designing
This is the best explanation,I have ever seen I can now understand Real time qPCR
Vincent Kibet please share
Thank you so much Arpan! I've never understood qPCR like I did in this 8 mins video!
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Omg i love you! Really wish my professor explained this well.
Lots of love from India 🇮🇳. Please share my channel link with your friends and help me to reach big audience
Love your channel and the way you make difficult concepts easy.
I had a question about primer property checking using Primer-BLAST.
I have a primer that contains degeneracy/wobbles and I want to check its properties such as Tm, self-complementarity, self 3’ complementary using the primer- blast…
However, when I use the primer Blast and search, it states that “ambiguity letters other than N are not allowed in the primer sequence”
So that forced me to replace all the degenerate/wobble letters with N.
Would that still make the property results correct? Am I still getting the right answers if they are replaced with N?
Thank you so much, I would really appreciate some insight on this, as I can not find an answer anywhere.
Clear explanation. But do add some more text in your slides, like principle of the technique & lable each & every component in the slides. So that it will be much useful to us. Thank you.
Really glad to know it was useful. Please share my channel with friends. You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
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Great job, dude!
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This video is really well explained and helpful
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
If I get a annealing temperature 66.4 and got single peak of melting curve, can I use that annealing temperature? While the Melting curve temperature range is 65_95’c ….what you suggest?
Please make a video on 3' RACE and 5' RACE
than you man, it helps
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You are great teacher, !!!
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Very good lecture !
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The explanation was really good. N easy to understand. Thank you very much most of my doubts was cleared ❤
Watch the detailed video czcams.com/video/tH_ozcFwQ_Q/video.htmlsi=Y78Ps-hNVHvNBjoM
You are gonna touch the sky 🌙
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What is the need to do qpcr over pcr sir?
Watch this video to learn czcams.com/video/_pMCwREQ22I/video.html
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@@animatedbiologywitharpan this link is not opening sir
with PCR you collect RFU data once the 40 cycles are complete vs qPCR you collect RFU data across all cycles; hence why q stands for quantitative
Thank u so muchhhh sir
Is sybr green specifically attached with the target gene?
No no it can bind to any double stranded dna, but the pcr will selectively amplify the target gene so in the reaction tube there will be huge quantity of target with which sybr green can bind
Can it is use for rabies detaction...
thank you so much was very very helpful
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Best explanation about real time pcr.... Really good 😍😍😍😍😍
Do subscribe to my other channel czcams.com/channels/4IpyopsGWSjaPACNTZLuqg.html
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@@animatedbiologywitharpan of course sir👍👍👍👍👍👍
Can qrt pcr be used to determine the exact number of viruses that have infected the sample?
No …a plaque assay can determine that.
Very nice video, but it would be great if there was subtitles
Thanks for your feedback
Sir I am checked hiv RNA quantitative pcr test result is target not dedicat meaning is negative hiv ? This is conclusive result ? Pls answer me😒
What you mean by base line
Like the first florescent ever or when it is above the threshold number?
The baseline of the real-time PCR reaction refers to the signal level during the initial cycles of PCR, usually cycles 3 to 15, in which there is little change in fluorescent signal. The low-level signal of the baseline can be equated to the background or the “noise” of the reaction
@@animatedbiologywitharpan thanks alot dr
Hi. Is there a cell type qPCR only uses? Or is available to use with all cells?
For all cell types
He is a legend ....ARPAN is a LEGEND ....
I am not sure that if he is an ( whatsoever you have mentioned ). I know him personally, he had to do a lot of struggle in his life . Thanks for your compliments though.
@@animatedbiologywitharpan rectified ..felt sorry
sukriya , good video
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Well understood thank you
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Good explanations.......what if the ct value of control and treated sample are same say 26 for both. Does it mean that the transcript level is same in both.
Absolute CT value does not mean any thing the delta CT value is the key measure.
Thank you......
thank god your channel exists ahha
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Excellent work
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sir abhi window period mai hu sir actually next month mai canada jaaa rha hu pr m glti se hepatitis C ke contact mai agya hu … sir confirm nhi horha….. pls btao sir kuch 25 febuary ko needle use ki thi hep c postive ldke ki sir fr 10 din baad hcv pcr rna quantitative test krwaya …. Usme not detected aya … sir plss btao next test kab karwau ki accurate aajaye jldi mne canada jana h..
pls help sir🙏🙏🙏
Can anyone suggest me a book from which I can learn about Real time pcr
Why did you like the comment, but didn't suggest any literature to this person?
Introduction to biotechnology. By William J. Thieman and Michael A. Palladino.
@@danaahmed8158 Thank you
@@sadiarajpoot7433 😊😊😊
Dorak's real time PCR
thank you
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great
Please share among friends and help me to reach big audience
Thank you
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Sir i recently done test rna pcr the sample was collected in heparin tube will this affect my result? Or the person carrying this test how will he know about inhibitors ?it was qualitative test.
It's very difficult to tell, it depends what you want to detect ultimately
@@animatedbiologywitharpan it was related to virus detection about sti it was sent to thyrocare lab?
I cant find the Value analysis video
It's a paid content now
Awesome bro nice
Thanks glad it helped you
I used to watch your videos bro this year i have secured AIR 256 but now I am pursuing biotechnology 2nd year i hope at the final year rank will be up
Sure you would do great....stay optimistic
Thank u bro😇
Bro will u make a video on NMR (conceptual lecture)
Thank you sir
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is mlpa a qpcr type?
Could you please tell me the full form of mlpa
is qpcr same as gradient pcr?
No no gradient PCR is completely different from qPCR
@@animatedbiologywitharpan what's the core difference please
I will upload a video on that. Just wait till tomorrow.
@@animatedbiologywitharpan is bio rad the only manufacturer of gradient real time pcr?
How you got melt curve chart?
you can add melt curve after the standard PCR cycle
@@animatedbiologywitharpan can you explain more how?
@@animatedbiologywitharpan i use ncbl website
How can I contact you ,do you have what's up for contact sir?
Now we talked .
sir can i get this ppt?
You can buy it from our collection @3$ . Email us at arpanparichha1994@gmail.com if you want it. We will let you know how to do the payment
The inventor of PCR, Kary Mullis, said that quantitative PCR was an oxymoron.
why
You mean you are torturing me electrinclyim a living man not an lab rat is this why my brain has been damaged
too many ads
czcams.com/video/tH_ozcFwQ_Q/video.htmlsi=p9BlewlmahXyZbEt qpcr details
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