Total Plate Count (Total Aerobic Bacterial Count)_A Complete Procedure (BAM, Ch-3)

Total Plate Count (Total Aerobic Bacterial Count) is a very important Microbiological testing parameter for Food, Feed, Water, Environmental and other samples analysis. This video represents a detailed procedure for the enumeration of total bacteria in a sample.
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[@dasarisailaja-uv3lq]

Very good explanation thanks a lot

[@efeodjegba4215]

this is amazing thank you

[@gousiagulzar7002]

Your videos are saviour for people like me who are not from pure microbiology field 🥹

[@MicroChemsExperiments]

Thank you so much.

[@SachinYadav-sk3fh]

Wow very useful video
Thank you for your all the videos.
Love you ❤️

[@MicroChemsExperiments]

Thanks

[@mahnoorsohail8479]

best video 😍

[@officiallighthouse]

Excellent, very clear and to the point presentation.

[@MicroChemsExperiments]

Glad you liked. Thanks

[@biannekaulf9221]

Wow, thank you very much for sharing this.

[@MicroChemsExperiments]

You are welcome

[@KhalidSaleem-un8hd]

Wow it's Amazing and helpful❤

[@MicroChemsExperiments]

Thank you

[@afolabipeter7073]

Am blessed to have watch this❤

[@MicroChemsExperiments]

Thank you

[@deedee4527]

Thank you so much, well explained.

[@MicroChemsExperiments]

Thank you

[@user-bu1ki1sm5b]

can you help me with what TPC count should be considered in acceptable range
for frozen fruits and vegetables?

[@zevanoc.sibarani8295]

Can you tell me what is the standard method you use to do this procedure? Thank you 🙏

[@asmeretkidane4269]

very nice presentation continue with clear

[@chauhanasmita4651]

It's really helpful thank you

[@MicroChemsExperiments]

Thank you

[@nankwopmarkus4884]

Why using phosphate buffer?
Can one use distilled water instead?

[@ashapatil992]

Very nice procedure thank you

[@MicroChemsExperiments]

Thank you

[@rakibhassan2451]

Your video's always awesome 😘😘😘

[@MicroChemsExperiments]

Thank you

[@GMCLABORATORY]

Why we didnt used first formula? is there any reference to take cfu between 50 to 250 cfu?

[@fathimasidhiky4412]

very well explained

[@MicroChemsExperiments]

Thank you

[@purushotamgorasiya7109]

Very good and in detail explanation. Can you make same video for total coliforms. How to count and which method is highly accurate. MPN OR Plate?

[@MicroChemsExperiments]

Video for coliform is already uploaded. Please find from the video list of this channel. Plating method is more accurate

[@hamidnazari2694]

Love you 😀🙃🙂😁😃 its so usefull

[@MicroChemsExperiments]

Thanks

[@mdsajibhossain747]

Same methods can we use for Drinking water or juice analysis?
One more request if you possible please upload determination of yeast and Molds and Magnesium analysis in Drinking water.
Thank you very much.

[@MicroChemsExperiments]

Same method is for Drinking water or juice analysis.
We will make video for yeast and Molds and Magnesium analysis

[@ervingopo5734]

Thank you for sharing such a great video. May I know why plates are left opened while solidifying? Thanks.

[@MicroChemsExperiments]

To avoid condensation of water vapour on the lid of the dish because the media is still hot

[@setiawanalwisius-tu6vx]

Good video, i have quation while open plate lid until the liquid agar be solid have u ever the plate countaminated, what u to do to make sure that open lid plate agar until solid could'n countaminated ?

[@MicroChemsExperiments]

There is no chance of becoming contaminated inside the biological safety cabinet. So, use a calibrated Biosafety Cabinet and monitor it's microbiological quality by settle plate technique periodically.

[@balurajnadeesh2788]

Very useful vdo

[@MicroChemsExperiments]

Thank you

[@abdallahadam9734]

I loved it

[@MicroChemsExperiments]

Thanks

[@IzzyRoxy]

Very clear explanation, really helped me alot thankssss 🥹💖

[@dessienatalia1193]

Is that phosphate buffer can be used as diluent solution for all products ? Including carrageenan products ?

[@MicroChemsExperiments]

Yes

[@ervingopo5734]

Hello, thank you for sharing such great video. May I know why plates are left opened while solidifying?

[@MicroChemsExperiments]

To avoid condensation of water vapour on the lid of the dish because the media is still hot

[@microbegist7918]

I find this content helpful as a student doing her project.

[@paleshwongshrestha2798]

Plz make a video of testing milk powder fat, solubility index, wettability acidity bulk density , sediment test and all ..regarding the test needed for milk powder

[@MicroChemsExperiments]

Noted

[@user-xs2gk3yj9t]

nice peresentation

[@MicroChemsExperiments]

Thanks

[@asmeretkidane4269]

how many sample is done in the same time ?

[@yosam9393]

sir can you make video on porosity,bulk and true density, car's and compression index for pulses and cereal determination?

[@MicroChemsExperiments]

Will make leter

[@999_finesse]

I’m stuck at a calculation. My plate count is 52 and Dilution Factor, 10^-3
Volume of sample plated is 0.1ml. My final answer is 5.2x10^4CFU/ml
The accepted count is

[@MicroChemsExperiments]

There is no wrong in your calculation. Your sample contains higher load of bacteria which exceeded the standard limit

[@venkybunny1554]

would you please make a video on the hanna ph meter

[@MicroChemsExperiments]

Already made. Link: czcams.com/video/PRxJgmzd5jE/video.html

[@rajuahmmed1257]

Could you please upload the quantification video of beta carotenoid from carrot sample by UHPLC?

[@MicroChemsExperiments]

Noted.

[@HK-Media1]

Nice

[@MicroChemsExperiments]

Thank you

[@manjiripatwardhan336]

Can you please make video on MPN method for detection of Esheresia coli.

[@MicroChemsExperiments]

Noted

[@yen4310]

What if my acceptable counts is in the first dilution, will the formula be C1/1xd?

[@MicroChemsExperiments]

Yes. Please share your final results here

[@kotaspecial9047]

Can you guide me how to test tri basic lead sulphate testing method

[@MicroChemsExperiments]

Will upload soon

[@rjedey658]

Mam can I use this technique for khoaya,Chenna or any milk based sweets?

[@MicroChemsExperiments]

Yes you can

[@mani-ls7kj]

Please share the determination of ammonium acetate assay method

[@MicroChemsExperiments]

Noted

[@callistaemeali5052]

Can I use plate count agar and peptone water? Is that acceptable?

[@MicroChemsExperiments]

Yes. You can use.

[@rutujajadhav9446]

Which medium should be used total viable count of milk

[@MicroChemsExperiments]

Plate Count Agar

[@gousiagulzar7002]

Can we use peptone instead of buffer you mentioned

[@MicroChemsExperiments]

You can use peptone salt solution

[@suzaava3691]

In the formula, how did you get 1 and 0.1 please.
Thank you

[@MicroChemsExperiments]

The formula is given by BAM, CHAPTER 3

[@ynen_405]

Can i use Nutrient agar and peptone water in cookies? Is it acceptable?

[@MicroChemsExperiments]

You can use and will get results

[@gargireghunath2114]

Is this method used for testing shrimp?

[@MicroChemsExperiments]

Yes, applicable

[@karthiyayanikrishnan5675]

How can i calculate cfu if i got less than 25 colonies in 10^1 dilution?

[@MicroChemsExperiments]

Make report as

[@khushiyadav6723]

Does the same method used in industries?

[@MicroChemsExperiments]

Yes. You can use

[@Madhuu151]

If there is a video that calculates the histamine level in a fish sample using the Elisa method, can you please provide the link?

[@MicroChemsExperiments]

No video was made on this test yet.

[@karntokaali9778]

Can I replaced by nutrient agar media?????

[@MicroChemsExperiments]

You can but without any reference

[@vikashganvit3548]

Ma'am how to do antimicrobial activity of polymer films?

[@MicroChemsExperiments]

Same as shown in the video

[@vikashganvit3548]

@@MicroChemsExperiments polymeric film is solid

[@MicroChemsExperiments]

@@vikashganvit3548 dissolve in solvent at a certain concentration. Make a bore hole into the agar surface. Add 50 microliter of sample solution to the bore hole

[@akramasgari]

Best 👌

[@sashikalawanasinghe1021]

Can this procedure use to butter sample?

[@MicroChemsExperiments]

No

[@sashikalawanasinghe1021]

What is the procedure used to butter sample ? 😢

[@DESIVOCALTV]

Why we make two/more petri plates of same dilution ?

[@MicroChemsExperiments]

There is a possibility of cross contamination in one plate. 2 plates from each dilution were chosen to avoid the doubt of cross contamination of the PCA plates

[@saurabhdeshmukh2605]

Of which sample you had calculated this cfu

[@MicroChemsExperiments]

Soyabean meal

[@zarqaarshad411]

Plz upload video of campylobacter, staphylococcus,fecal coliform protozoa and helminths

[@MicroChemsExperiments]

Noted

[@MrLongKR]

hi. may i know the reference for this procedure?

[@MicroChemsExperiments]

BAM, Chapter 3

[@safeedhasaidalavi9786]

Can you pls tell the limit for total bacterial count in the case of biscuits

[@MicroChemsExperiments]

100CFU/g

[@neethuaneethua941]

I have a doubt regardung the buffer preparation.is the 500 ml buffer convert into 1000ml and from that only 1.25 ml is then add to 1000ml distilled water for working buffer dilution preparation?please rectify my doubt as soon as possible.

[@MicroChemsExperiments]

Buffer preparation is correct. Please read the reference protocol (BAM, Chapter 3) to justify it. Thanks

[@neethuaneethua941]

​@@MicroChemsExperimentsthank you

[@neethuaneethua941]

Can you please upload a video about enrerobacteriacea (IS 17112 part ll

[@MicroChemsExperiments]

@@neethuaneethua941 noted

[@neethuaneethua941]

I got only 4 colonies in 10^1 dilution(sum of 2 trial plates).what will be the CFU/ml?please reply mam.its very urgent for me.

[@meenageeths9730]

Very easy planas

[@MicroChemsExperiments]

Thanks

[@bulakochebo7636]

please whose protocol is it?

[@MicroChemsExperiments]

Reference Method is already mentioned. Please check again

[@sonal3124]

Which method use? Pls send the method name

[@MicroChemsExperiments]

Reference is given in this video

[@degontoislam925]

could you please share the reference for this work, thank you

[@MicroChemsExperiments]

BAM chapter 3

[@degontoislam925]

@@MicroChemsExperiments thank you

[@anbarulhaque4720]

How to convert cfu/g to cfu/ml

[@MicroChemsExperiments]

These two units are same. CFU/g is used for solid sample and CFU/ml is used for liquid sample

[@anbarulhaque4720]

3.21 cfu/g is my reading
.: Then how many colonies are there

[@MicroChemsExperiments]

There are about 3 colonies in 1g of your sample.

[@ankitsaxena8738]

Ky ap ye video hindi me nhi bna skte hain aur 🙏❤️

[@megharaghuwanshi4102]

How to perform B.et test

[@MicroChemsExperiments]

Please write the full name of the test

[@somnathchanda1250]

Use natural accent

[@MicroChemsExperiments]

Ok. Will try

[@dr.shashankbaghel]

thanks but you should avoid fake accent

[@anbarulhaque4720]

3.21 cfu/g is my reading
.: Then how many colonies are there

[@MicroChemsExperiments]

There are about 3 colonies in 1g of your sample.