Four Quadrant Streak procedure - How to properly streak a Petri plate for isolated colonies

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  • čas přidán 15. 06. 2024
  • In this video you will learn how to use proper lab technique to streak a Petri plate for isolated colonies using the four quadrant streak method. To learn more about Hardy Diagnostics visit our new website. 👉 go.hardydiagnostics.com/yt-ha...
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    00:00 Intro to streaking an agar plate
    00:21 What to know before beginning
    01:01 Preparation
    01:50 Four quadrant streak diagram
    02:29 Types of loops
    03:28 Collecting a sample
    03:43 How to do a four Quadrant Streak
    04:36 Using a swab
    05:28 Incubating the plate
    05:43 Using a plastic loop
    06:24 Close and ordering info
    Do you prefer reading when learning? Here is our blog:
    hardydiagnostics.com/blog
    Filmed and Edited by Brian Tom
    #Streakplate #Bacteria #Microbiology #HardyDiagnostics #4quadrant
    Tags:
    quadrant streak plate, streaking agar plate, agar plates, streaking agar plates, quadrant streaking, how to streak on agar, what is a four quadrant streak, four quad streak, how to streak a petri plate, petri plate streaking, streak plate method english, quadrant streak, spread plate technique, streek agar plate, what is a four quadrant streak, what is a four quad streak, help doing a four quadrant streak, study four quadrant streak, exam four quadrant streak
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Komentáře • 109

  • @Ms1101997
    @Ms1101997 Před rokem +231

    I’m a new microbiology student and I’ll do this for my tomorrow’s lab 😅 I was confused but thanks god I found this video

    • @HardyDx
      @HardyDx  Před rokem +16

      We love helping new micro enthusiasts! Feel free to browse our other tutorials for your class too!

    • @winispecial
      @winispecial Před rokem +5

      "Thank God you found this video "

    • @CrankyRayy
      @CrankyRayy Před rokem

      @@winispecial " " Thank God you found this video " "

    • @mdraihan1582
      @mdraihan1582 Před 3 měsíci

      What's your country name?

    • @Ms1101997
      @Ms1101997 Před 3 měsíci

      @@mdraihan1582 I’m from the US, California

  • @notjeb_9409
    @notjeb_9409 Před rokem +20

    its so wild how a lot of ppl commenting here r college students or professionals n here i am watching this for our lab activity in my high school biology class. cool video, helps a lot

  • @Faceless_Chat
    @Faceless_Chat Před měsícem +1

    Very helpful. I have a "unknown bacterium" assignment that starts today. This really helped bc I already forgot you have to sterilize the loop between streaking...

  • @daliadivicpuskarski7453
    @daliadivicpuskarski7453 Před rokem +28

    Yesteday I streaked a Petri dish for the first time in my life. I've watched this video before doing it and it really helped me 🧫 Thank you 😊

    • @HardyDx
      @HardyDx  Před rokem +4

      That's wonderful. Glad it helped!

  • @ry_n007
    @ry_n007 Před měsícem +1

    thank you for saving my ee broski 🙏🙏

  • @sloane.2747
    @sloane.2747 Před rokem +14

    This was so much fun when I did it, microbiology was my literal favorite class my first semester of college

    • @HardyDx
      @HardyDx  Před rokem +2

      Glad to hear it! Keep on learning!

  • @Dananq98
    @Dananq98 Před rokem +14

    So useful description, thanks a lot 👏🏻

  • @nina-ge6mn
    @nina-ge6mn Před rokem +19

    I love microbiology and honestly its just so satisfying to watch people streak plate
    I'm doing a microbiology project that involves e. Coli K-12 plating and im so excited

    • @HardyDx
      @HardyDx  Před rokem +5

      Nina, we love projects for the tiny humans! Please feel free to reach out to your local sales rep for product, we'd love to help out the next generation of micro-microbiologists!

  • @user-ou9kq6xf7k
    @user-ou9kq6xf7k Před 4 měsíci +1

    It's self explanatory and the visuals, helps for easy remembrance.
    Thanks.

    • @HardyDx
      @HardyDx  Před 4 měsíci

      You are very welcome. I am glad our video was useful for you.

  • @DanTheisen
    @DanTheisen Před 2 lety +31

    This is an excellent video. Thanks, Hardy for showing us how this is properly done. Much more to it that a squiggle of bugs on a petri plate!

    • @HardyDx
      @HardyDx  Před 2 lety +2

      Glad it helped!

    • @DanTheisen
      @DanTheisen Před 2 lety +1

      As required by law, I’m disclosing that I was employed by Hardy Diagnostics at the time of this comment.

    • @Sean-ur4cb
      @Sean-ur4cb Před rokem +1

      @@DanTheisen what did you sign that told you to disclose this info?

    • @DanTheisen
      @DanTheisen Před rokem +2

      I don’t recall where I saw it but this is, as far as I know, meant to prevent large companies from having their employees promote their products as if they are customers. I have not signed anything from the company compelling either a comment or a disclaimer about the comment. The law as I read it made sense to me from a consumer protection point of view, which is why I happily added it.

    • @Sean-ur4cb
      @Sean-ur4cb Před rokem +1

      @@DanTheisen ahh I see, thanks for the reply. Have a good day!

  • @leannajulien4175
    @leannajulien4175 Před rokem +5

    I didn't know I have place it in the heat everything I learned something new. Thanks

    • @HardyDx
      @HardyDx  Před rokem

      We're always here to help you learn! We have a great Technical Services team should you ever have questions!

  • @clairem290
    @clairem290 Před rokem +7

    I'm a college instructor and I'm sending this video to my students before they do their first-ever cultures!

    • @HardyDx
      @HardyDx  Před rokem +3

      We love our instructors and our students! Feel free to reach out to us should you need materials for your students as well!

    • @Flowers12660
      @Flowers12660 Před 3 měsíci

      Well as an instructor why don't you show your students this yourself? Isn't this supposed to be your job to teach? It would be alot better. I really dislike and hate how college so called instructors and lecturers are relying so much on youtube videos instead of doing the work themselves.

    • @clairem290
      @clairem290 Před 3 měsíci

      I did streak some plates myself, which came in handy later. However, a nice CZcams video they could watch the night before was also really helpful. To make up for your previous instructors who didn't explicitly show you enough things, I will now show you how to spell the phrases "so-called" and "a lot."

  • @ayesha-it5yw
    @ayesha-it5yw Před rokem +4

    I learnt this method today in my microbiology lab.

    • @HardyDx
      @HardyDx  Před rokem

      That's great Ayesha! Keep up the proper four quadrant streak method, you will be a pro in no time!

  • @jessical4866
    @jessical4866 Před rokem +14

    I’m not a teacher or a student… why am I so fascinated with this? 😂

    • @HardyDx
      @HardyDx  Před rokem +9

      I mean we can't blame you, we do this for a living. Our whole lives are this, welcome to the obsession!

    • @GaneshKunayak
      @GaneshKunayak Před rokem

      Who are you 🤣😂

    • @affiliatemarketinginnigeri7617
      @affiliatemarketinginnigeri7617 Před rokem

      I'm a passer-by as well and I am entranced ❤️

  • @KHALILLABSKILLS
    @KHALILLABSKILLS Před rokem +1

    I really appreciate you sir.

  • @johnson1262
    @johnson1262 Před rokem +4

    My universities professor , didn’t tech to put the petri disk , up side down when going into incubator. Thank you for sharing.

    • @HardyDx
      @HardyDx  Před rokem +5

      Oh no! When media is incubated "upside down" like that, with the media facing upwards, condensation can accumulate on the face of the agar and inhibit proper growth and developing colonies. If you invert your plates, you'll definitely have better-looking plates than the rest of your class!

  • @ruchika_899
    @ruchika_899 Před rokem +2

    Gorgeous explanation 💥❤️

    • @HardyDx
      @HardyDx  Před rokem +1

      Thank you, glad you liked it!

  • @AL_ayyubi1137
    @AL_ayyubi1137 Před rokem +4

    I'm medical laboratory technician(MLT) and 11 lecture is included for simple technique in tomorrow thank you for this video
    I appreciate it 🤩

    • @HardyDx
      @HardyDx  Před rokem +4

      You are welcome, I am glad the video was helpful. Keep up the great work as a MLT. You are important.

  • @tayyabirshad3029
    @tayyabirshad3029 Před rokem +2

    Today may microbiology practical exam it's help alot!!🥰🥰🥰🥰

    • @HardyDx
      @HardyDx  Před rokem +1

      Glad to hear the video was useful. You are very welcome. Good luck with your exam. You will do great!

  • @vennelagovindugari5420
    @vennelagovindugari5420 Před 2 lety +5

    Perfect!!

  • @ruchika_899
    @ruchika_899 Před rokem +1

    Excellent explanation ❤

    • @HardyDx
      @HardyDx  Před rokem +1

      I am glad our video was useful for to you.

  • @KHALILLABSKILLS
    @KHALILLABSKILLS Před rokem +1

    It's absolutely Amazing sir.

    • @HardyDx
      @HardyDx  Před rokem

      Thanks, it's our pleasure.

  • @KHALILLABSKILLS
    @KHALILLABSKILLS Před rokem +1

    It's well explained.wow

  • @Manthab
    @Manthab Před rokem +1

    Don’t know how I got here at 1am but it was fascinating and informative if I ever need to know this I’m covered😁

    • @HardyDx
      @HardyDx  Před rokem +2

      Welcome down the rabbit hole of microbiology, Mantha! We've got a whole channel of videos if you ever need more obscure information to get you even more hooked 😃!

  • @anycamenzind
    @anycamenzind Před 6 měsíci +1

    Will there be a video available that talks about the microscope and Khoeler illumination? Unfortunately, part of the practical microbiology and laboratory sessions were taken in times of pandemic. Likewise, I would greatly appreciate if you could explain how the UFC colony counter works. Your explanations are great!
    Thank you 🙌🙌🙌

  • @jennisagala1263
    @jennisagala1263 Před rokem +1

    thanks a lot, i hope, my thesis will be finisih

  • @1alex92
    @1alex92 Před rokem +5

    Wish I seen this 2 weeks ago when I had a test!
    ...nevertheless.. I just subscribed ☺️

    • @HardyDx
      @HardyDx  Před rokem +1

      Welcome to the online Hardy family! Stay tuned for more great content soon to come!

  • @nithinmuluka7987
    @nithinmuluka7987 Před rokem +2

    Thank you sir very nice video... Pls upload more videos

    • @HardyDx
      @HardyDx  Před rokem

      Yes, stay tuned. More video are on the way!

  • @todddillerson4053
    @todddillerson4053 Před rokem +6

    I do this every day for the hospital I work at :)

    • @HardyDx
      @HardyDx  Před rokem +1

      That's great! Heroes wear lab coats not capes. Which hospital do you work at?

    • @Hellamoody
      @Hellamoody Před rokem +1

      @@HardyDx boundaries Dwight!

  • @apokalypthoapokalypsys9573

    I'm in an IT MSc but this is perfect ASMR

    • @HardyDx
      @HardyDx  Před rokem +1

      Love it! That's a whole new industry we should break into!

  • @Szaisthatyou
    @Szaisthatyou Před rokem +1

    I love my major ❤

  • @FarzadSalim
    @FarzadSalim Před rokem +1

    thank you

  • @RaiSaysS
    @RaiSaysS Před 2 lety +5

    Thank you

  • @AliyuNanaaishat
    @AliyuNanaaishat Před 2 měsíci

    Please can I see a video that will teach me more on how to carry out indoor air quality using SDA and PDA

  • @user-ot7km4px5n
    @user-ot7km4px5n Před 7 měsíci

    How do you report colony growth on a 4 way streaking? along with its size, shape, transparency, consistency, and color.. Thanks

  • @ceciliaovansa2738
    @ceciliaovansa2738 Před 4 měsíci +1

    Nice one

    • @HardyDx
      @HardyDx  Před 4 měsíci

      Thanks for watching

  • @nangsanbhalangblah333
    @nangsanbhalangblah333 Před rokem +3

    Great vid, I've a question though. Why is the media red in color? Is there a different type of agar used?

    • @918mounika7
      @918mounika7 Před rokem +2

      It's probably blood agar medium, blood added to regular nutrient agar medium

    • @HardyDx
      @HardyDx  Před rokem +3

      Nangsanbhalang Blah, Great question about the coloration of the agar! This was streaked on our Blood Agar, an all-purpose growth agar with hemolytic properties. Some organisms will hemolyze the blood in the agar, leaving clear zones around the colonies, something we won't see on traditional TSA. Hope that helps!

  • @user-bw1uz7oy1w
    @user-bw1uz7oy1w Před 6 měsíci

    Quand utiliser la methode en stries?

  • @sunny4483
    @sunny4483 Před rokem

    Can we not streak circular on plate instead of streaking zig zag? I'm curious

    • @MrDubbindan
      @MrDubbindan Před 7 měsíci

      There is an instrument that does streak with a plastic "comb" after pipetting a volume on a plate and it does make circles ... The Previ Isola

  • @M313_1
    @M313_1 Před 4 měsíci

    *ثانكيو* 🦦

  • @pastmonster446
    @pastmonster446 Před rokem +1

    Watching this after I took my lab practical exam, and I definitely fucked it up

  • @joannadevung4358
    @joannadevung4358 Před 2 měsíci

    If i accidentally tore the surface of the agar using the loop during streaking, will the bacteria colonies still able to grow?

    • @HardyDx
      @HardyDx  Před měsícem +1

      Colonies will still grow, yes. However, it is usually best to restart on a fresh plate as the puncture could lead to difficulties in reading/counting colonies or could cause unwanted areas of heavy growth. Don't worry though! Marring the surface is extremely common, especially in beginners. Keep practicing and you'll be able to do it as if it were nothing!

  • @mohanzm12
    @mohanzm12 Před rokem +3

    What do we call the thing she used to flame the loop ? 😅

    • @DadaClaire
      @DadaClaire Před rokem +1

      I'm curious too. I'm working with unicellular fungi and bacteria since time, streaking them to isolate but it's the first time ever I'm seeing that instrument.

    • @vanessadang4674
      @vanessadang4674 Před rokem +2

      It is called a bacticinerator :)

    • @DadaClaire
      @DadaClaire Před rokem +1

      @@vanessadang4674 Thank you!

    • @HardyDx
      @HardyDx  Před rokem +1

      @Mohan The tube the loop was inserted into is a special type of sterilizer. Placing non-sterile metal loops into this tube for a few seconds will sterilize these loops so you can re-use these again and again. Hope that answers your questions!

    • @mohanzm12
      @mohanzm12 Před rokem +1

      @@vanessadang4674 wow .thank you

  • @SOPHIA-yf7ws
    @SOPHIA-yf7ws Před 9 měsíci

    A++++

  • @GaneshKunayak
    @GaneshKunayak Před rokem

    Bdw i think this must be carried out inside a laminar air flow

  • @kasisfile
    @kasisfile Před rokem

    😮

  • @hebahariri9936
    @hebahariri9936 Před rokem +1

    Hello, I'm a microbiologist and a content creator on Instagram educating microbiologists in Arabic, can I translate your video and post it on my account? with sharing your video link of course. thank you.

    • @HardyDx
      @HardyDx  Před rokem

      Yes, that would be fine as long you tag and credit Hardy Diagnostics in your video description and link it back to the original video. Keep up the great work in microbiology.

  • @chrisk6469
    @chrisk6469 Před rokem +1

    Watching them tear up the agar like that was a bit painful ngl

  • @mtado4933
    @mtado4933 Před 17 dny

    yeah i fucked it up so badly

  • @lilianmumbo880
    @lilianmumbo880 Před 2 lety +3

    5 method of preparing specimen

  • @akritikuikel8506
    @akritikuikel8506 Před 8 měsíci

    how to get a job or internship of microbiology in europe. I have done bachelor in microbiology and working in a restaurant since 4 years. Did not even get any inernship. Already forgot all the techniques and skills in these 4 years . sad sad

    • @BlueFox61
      @BlueFox61 Před 2 měsíci

      Build a portfolio of your interests and maybe your thesis paper too. It's hard to get an internship now

  • @user-me1hh5po6h
    @user-me1hh5po6h Před rokem +1

    عربي وين

  • @sriningsih4808
    @sriningsih4808 Před rokem

    kangen haha

  • @alvinxyz7419
    @alvinxyz7419 Před rokem

    I thought the title said steak

  • @John-fr6wo
    @John-fr6wo Před rokem +5

    For God so loved the world that He gave His one & only Son, that whoever believes in him shall not perish but have eternal life. For God did not send His Son into the world to condemn the world, But to save the world through Him. (John 3:16-17)✝️