How to Prepare a batch of Petri Plates from Dehydrated Culture Media

Take an in-depth look at how petri dishes are prepared using dehydrated culture media. In this example, we are preparing Hardy Diagnostics Criterion™ MacConkey agar, a medium intended for the isolation and differentiation of Gram-negative bacteria. Use our preparation tips to prevent bubbles or other flaws from forming in the agar. To learn more about Hardy Diagnostics, visit our website. go.hardydiagnostics.com/yt-ha...
Hardy Diagnostics manufactures culture media and rapid identification kits for microbiological testing in clinical and industrial laboratories.
Hardy Diagnostics CRITERION™ MacConkey Agar
hardydiagnostics.com/catalogs...
Dehydrated culture media, CRITERION™:
hardydiagnostics.com/criterion
See our complete list of catalogs in the link below
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We are 100% employee owned and have been serving microbiologists for over 40 years. To learn more about Hardy Diagnostics and the products we offer, visit us at hardydiagnostics.com/
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00:00 Intro
00:24 What you will need
00:54 Getting started
01:18 Weighing the powder
01:30 Select a flask and add ingredients
02:16 Place flask on hot plate stir
02:54 Autoclave media
04:08 Remove from autoclave and cool
04:32 Adding supplements
05:01 Pour into plates
05:31 Tip: removing bubbles
05:37 Allow plates to settle
05:42 Storing plates
06:21 Sterility check
06:46 Cleaning
07:00 Close
Filmed and Edited by Brian Tom
#HardyDiagnostics #PetriPlate #microbiological

[@ZainabLambe-dj5hx]

Thank you for the video

[@NiyibugingoValentin-dk7sk]

Thank your brother is here with me and I will get it more information

[@Aakif862]

Love from India ❤

[@BAD5RO]

Informative

[@JimohRahama-ur6kz]

Thank for the video, I really benefit from it

[@HardyDx]

You're welcome Jimoh Rahama! I'm glad you found it helpful

[@antonsorkin]

This is a very good tutorial, thank you!

[@HardyDx]

Glad it was helpful!

[@MargretWanjiru-yo1zc]

❤ easy to understand good work

[@kaelthuzad4640]

thank you for the video , i usually never autoclave the media , specially not the selective. i test a small quantity for contamination and if it is okay i do not autoclave the , i just heat it for 10 muntes after the agar has totally melted and it does the job.

[@HardyDx]

For sterility assurance, we do recommend autoclaving the media. If you have any questions or want to dive deeper into the "why" of it, feel free to reach out to our Technical Services team at [e-mail], they're an amazing team who'd be happy to explain more!

[@mirhina8727]

Thnks for such useful video

[@HardyDx]

I am glad it was helpful.

[@user-mj4co3ll6t]

4:34 Is it okey to add the supplement outside of the clean bench?

[@ceciliaovansa2738]

It was nice watching

[@HardyDx]

Thanks!

[@Vijay-ol5se]

Should we add additional amount of agar agar for solidification?

[@md.mehedihasansiam4489]

Make a video of spread plate & pour plate method.

[@user-ch4wv4nn3c]

Очень интересная плита для приготовления сред

[@miaab7143]

Is it ok to autoclave for 1 hour and a half??

[@salahaldinmohamed6959]

Thanks from Libay

[@HardyDx]

You are very welcome!

[@redvelvet3479]

I made cetrimide agar and there are moisture on media but idk why

[@user-hm2rl9ef5w]

Why did you put the hose inside the autoclave into Erlen?

[@HardyDx]

Thank you for your question. The hose is actually a temperature probe. As the video explains the probe can be placed directly into the media (which is in the Erlenmeyer flask aka “Erlen”). As the directions state: “Place the flask of boiling media into an autoclave to sterilize. If your autoclave is equipped with a temperature probe, the temperature probe can be placed directly into the media and the cycle parameters will be based on the readings of the probe.”

[@coffee_sloth]

Why does boiling for too long damage the media, but autoclaving at 120 degrees doesn't?

[@selenabulanart]

You can boil it longer than one minute, we do it all the time with no issues. However bubble formation should be avoided

[@miltonkunda8912]

Why can't I work in one of your labs

[@raomasoodahmad1221]

❤❤❤🎉

[@roshanjannath6954]

How to pop up bubbles if don't have flame.....

[@blueraine13]

try using a pipette :)

[@HardyDx]

You can also try misting the inside of your empty flask with 75% ethanol, see if this helps!

[@ChrishaMacreeba]

Sterile Loop may help you out

[@kaelthuzad4640]

quicjly blow torch it , i use it in my lab

[@LandonStrauss-hc1sc]

Sounds like John Lithgow.

[@majumundurchanel2908]

gak ngecek ph mas?

[@HardyDx]

Good eye! We do actually check the pH of all our media for specific pH ranges. That video is in the works, and is coming soon. Stay tuned!

[@user-ju8jk1kl5w]

You are make a only false media make a real culture media

[@AmanExplorerBoy]

What do you mean by that