Serial dilutions and pour plate technique

Serial dilution involves repeatedly mixing known amounts of source culture with (sterilised) liquid. 1 ml added to 9 ml gives a 10-fold dilution; 1 ml added to 99ml gives a 100-fold dilution. When fixed amounts of this dilution series are mixed with an appropriate agar and incubated, then different numbers of colonies will be obtained. By working back from an easily counted plate and using the appropriate dilution factor, the number of micro-organisms in the original source culture can be calculated.
In a pour plate technique, a small amount of inoculum from a broth culture is added by pipette to the centre of a Petri dish. Cooled, but still molten, agar medium in a test tube or bottle is then poured into the Petri dish. The dish is then rotated gently, or moved back and forth, to ensure that the culture and medium are thoroughly mixed and the medium covers the plate evenly. Pour plates allow micro-organisms to grow both on the surface and within the medium. Most of the colonies grow within the medium and are small in size and may be confluent. The few colonies that grow on the surface are of the same size and appearance as those on a streak plate.

[@techpinnaple3545]

Thank you so much for putting together this tutorial. It’s very helpful for my online Microbiology class 👏

[@yashikamittal5095]

Thanks for providing such a well explained video

[@gihan4550]

It was very clearly explained. Thank you for giving knowledge for us🙏.

[@blessingnelson4926]

Been looking for a video that talked about a solid sample. Thanks.

[@Woman_of_valor]

This was so detailed ,thank you so much ,keep up the good work 👏

[@usamahvictor479]

Thanks for the teaching I appreciate it's so explanatory and clear

[@37shivammaurya]

Very well explained each aspect in the video. Thank you.

[@wepsisaiah2238]

thankyou so much for simplifying this aspect, it was hectic for me..

[@maureenbandawe8808]

Thanks for a good explanation.

[@hafizumar5732]

Excellent method for juniors please carry on the world need u thanks for all

[@mightiestmouse8833]

GREAT VIDEO!!

[@sanirabiatualiyu9756]

Thank you so much this tutorial really help me alot as am using this method to isolate bacteria for my undergraduate project

[@nazaninmoazeni4227]

Thank you so much for excellent explanation

[@1024DS]

Thanks very well explained .a lay man can also understand this

[@pragatijha9363]

Thankyou fr this video.

[@user-wk2gp3tu8h]

Thank you very much

[@Alaa-cv8pj]

So helpful thank you ❤

[@olufemiidowu7840]

Very illustrative

[@amailyakter6589]

excellent explanation, thank you lecturer

[@amailyakter6589]

Glass rod spreading plate technique and pour plate technique 👉 can get same result?

[@ilyasulhaq50]

Thanks for very will explain.
we understand easily.

[@sevdaqurbanova8339]

Thank you!

[@chrisstemr7962]

Thank you 👍👍❤

[@sagaljp7148]

Thanks
Very clear

[@shubhashreejenajena9291]

Thank you

[@anushamalik8371]

best explanation

[@user-qq8lu4bk1r]

I love this

[@ravidhyani1011]

Good video

[@furkanmorgul3699]

useful video,thanks

[@DenizY18]

Great comment Furkan Purplerose ! Good luck.

[@furkanmorgul3699]

@@DenizY18 thanks to our teacher for these opportunities

[@mumamaureen102]

Very helpful

[@zemenuachamyeleh45]

thank u very mach guys

[@wt3377lyn]

for the solid sample, if I pour sterile liquid into the stomacher bag and stomach with the solid food, does that means after stomacher, the liquid itself is already 10^-1 ?

[@chrisstemr7962]

Thank you find this vedeo very helpful to me 🙏👍💗💗

[@muhammadasimkhankhattak97]

Great

[@vicidiah]

how many colonies are there in each petri dish?

[@sabasiddiqui1492]

Please suggest me which media is suitable for fecal coliform test by pour plate method and how we can prepare this media?

[@ShopperPlug]

That petri dish looks like a galaxy of different milky ways... Can you make a video on how to isolate multiple bacteria in one petri dish from a sample that contains multiple bacteria?

[@inactive392]

look for morphology ,color of the colonies in same petriplates.Mark it.Prepare nutriet agar and pour to petridish. Pick the colony and do quadrant straking .If not make slants in test tube.

[@ShopperPlug]

@@inactive392 That seems like a valid idea, thanks.

[@chathuanushki6331]

Very gd

[@johnpeegodiya9310]

Thank you somuch...its quite understood.

[@gauravgawas1987]

Hi Microbial zoo.......how much amount of diluted sample is mixed with melted agar medium...??

[@gauravgawas1987]

Thank you for the nice video...🙌🏼

[@rickym40]

How much inoculum to use to pour on the Petri dish

[@Kanishkzip]

0.1 preferably

[@rahuldevchauhan9451]

Will you please reply for this doubt that I'm having...
Why we always take the reciprocal of the dilution factor (eg:- 10^6 instead of 10^-6 )while multiplying it with no. of colonies for getting CFU count ?

[@amnamohayyuddin6326]

In 7:06 when last 4 dilutions were transfered to petri plates, how much quantity of dilutions was used to transfer n why only last four dilutions were transfered, not all?? Kindly answer

[@drehernandez05]

1 ml of sample.

[@anbarulhaque4720]

How to convert cfu/g to cfu/ml

[@unknown-zk7xx]

by such far distance from flame my samples got contaminated if i close pippete near flame while making dilution pipette burns what to do😣

[@chopyouup]

Minimize the time that the petri dish lid is off.

[@kocaksuricak9391]

ga bisa basa enggres, tugasnya kek gini, gils semangat kawan ngabrio kuu

[@kocaksuricak9391]

untung tadi gaada absen gan, rodok ndredeg yo lur

[@zulfayunita-3a321]

Tolong terjemahan ke bahasa indonesia!!

[@darshanavarma4399]

in this vedio bacteria has not been inoculated

[@yaswanth7950]

basically she is doing the exiperiment outside of the laminar airflow. then how would they say that do aseptic techniques automatically that will be got contamination. thank you. But thanks for the lecture its more informative.

[@milanbergh]

aseptic technique requirements are met by manipulating samples near the Bunsen burner

[@erastombilinyi1175]

Ni mulele oglo