🔬 How to stain cells with Methylene Blue | Amateur Microscopy
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- čas přidán 10. 07. 2024
- Here I use Methylene Blue (according to Löffler) to stain a variety of different cells: Cheek cells (epithelium), yoghurt bacteria, plaque from the teeth, and white blood cells.
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Concentrated Methylene blue is available very cheap at pet shops as it is used to treat fungal infections in fish.....Thank you Oliver for making such video....
@@NerdyNEET I have found 3g on ebay for under 10€
Also cheap is relative. If you work in a professional lab then the 400€+ for a vial of alexa fluor is cheap.
@@NerdyNEET Sure, the prices can vary a lot, but it doesn't have to be expensive. Also be aware that a few g of this stuff goes a long way, it really is quite potent, especially since you only need a drop per slide.
Of course if your only option is to buy 50g, then that isn't ideal.
Thanks bro...im using fish blue and green/yellow to put in my urine...its work..thanks atharva..
I actually asked you how to do this in you last video. Thank you very much!
Best chhanel for microbiology on CZcams 👍🏻
Thank you so much! Learned a lot of amazing things from this video 😁
Thank you so much for these great videos.
Very clear explanation, thanks!!!!
What an amazing channel! ♡
Hi thanks to do this video i use the Methylene Blue when i bought my first toy microscope and the second color is Mercurochrome and Methyl Green
nice video bro, you explained it very well
thats wonderful, good job
Hello, thank you for your great content! Could you please make a video about the different kind of dies, how they differ from one another and which ones you suggest for hobbyists? I am a bit confused about why there are so many dies and i bet their different color is not the only reason to use them.
Your video really helped me, Thank you🙂
Thank you very helpful!!
Great video! Thank you
Great information for anyone with a scope.
Thank you so much for the staining video....but I will be using pen ink since it is easy and cheap...🙃
Interesting video. Thank you. I have just purchased a microscope and will try this.
Sir great and nice and good job and Thank you 🌷🌷🌷🌷🌷
Can you use methylene blue to stain the onion root tips like you did in the last video? If so, what changes to the procedure would you need to make (particularly about the water bath and acid)?
Thanks!
Hi oliver
Can you please make a video on hematoxylin eosin staining with an animal tissue...
Im really interested to know how it is done. Also i couldnt find any good videos on youtube that do this.
Hi, thank you for this video. Can you do a video on the trypan blue?
Thank you for your amazing content! I got methylene blue from a pet store. This is used in aquariums to treat fungal infections in fish. It worked really well on cheek cells!
I tried this on onion cells, however, while the cell walls got stained, I could not see the nucleus. Any ideas on what could be going on/ how I can fix this?
Thanks sir
Regarding microscope donations, if you could include PayPal as a method of payment then it would make it a much easier and quicker process to make donations. Thank you for another interesting video. I bought some methylene blue a while back and needed this video to encourage me to use it with the chldren!
Methylene blue also is a photosensitizer, an efficient producer of singlet oxygen so that's another thing to have in mind when staining organisms, it can make them very sensitive to light in the 580-700nm range (orange to deep-red).
Is this the same stuff as Tryphan blue?
Is it good for keeping filters...after... Staining the specimen??
Hi, thank you for this video. Can you do a video on the trypan blue? the overall difference with methylene blue and health hazards. Thank you
I agree with your proposal
What dilution factor do you suggest ? Thanks!
hi bro am india my NAME is RAJA . i love your all videos . am big fan of you
Hi sorry for dustubing you but i would like to know if we don't have any stain like the methylene blue so how can we make one at home.
Is there an household alternative to methylene blue for the purpose of staining cheek cells?
The 50mls of Methylene Blue I bought is 1% aqueous. I assume that means the 50ml of liquid has 0.5ml of water dilution in it. However, does this mean it is a prepared solution ready straight from the bottle to use as stain with no need to dilute it further? There is no information regarding its use on the bottle I bought.
Is 1% aqueous solution concentrated, or equivalent to Loffler's?
I have a small bottle methylene blue bought from the pet shop :)
Do you ever stain using eosin? Do you have a video?
The Amscope kit I purchased comes with a small bottle of methylene blue "powder" with no instructions. Can someone enlighten me in how to use it? Thanks
Hello, How can we prépare thé solution of Méthylène bleu ??do we add alcool ethanol ?
I bought methylene blue with water solvent can i still stain cells for microscopy?
Can we do all this by safranin?
Much effective in covid patient treatment... 95 percentage recovery.... 👍👍👍
G6pd patients not allowed to tak mithenil blu..
How to stain yeast cell From colony by methylene blue,sir??
Yes❤️
Can I use methylene blue to see mitosis in onion root tips?
I put a drop of my own blood on a slide to observe it. I have done this many times before, and it is always interesting to see. Recently I left such a slide sample (small drop, no stain, has a cover slip) out for a few days to see what it would look like. I had no idea what to expect. I could not see any blood cells, but I did notice very interesting looking areas surrounding empty space, and these areas had a very clear high-contrast dark line. I am curious if anyone knows what exactly I'm looking at - is it blood plasma? What happened to the blood cells? Did they evaporate? I have tried to do some research online, but I can't find anything.
apoptosis
What filter on a microscope do I need to add to see cells stained with Methylene blue stain
No filter needed. Bright field is enough.
What other stains can you use for staining blood smears?
Graham's stain, but its a bit difficult to do correctly.
So how would you stain pond samples and or live organisms?
I would use a very low concentration of methylene blue. It is also used for disinfection of aquariums, so at a right concnetration it might not do much harm. generally it is not necessary to stain water samples as the contrast is generally high enough.
@@MicrobehunterMicroscopy can I use my methylene blue that dissolved in water to stain my cells? You used alcohol based methylene blue so I'm concerned that i bought the wrong one, i was also suspicious about it being 1 Litres but couldn't find ANY information on the internet.
So ... why aren't the red blood cells red? I can imagine that they are not the dark saturated color of the drop of blood itself but they seem to be completely colorless in your pictures? Does the methylene blue actually discolor them?
This is a great video. I like this kind of "how-to" video's. Keep them coming!
Thanks!
Becasue they are so thin that the effect of the red pigment hemoglobin is not visible. The light also goes through the cells and this therefore looks different then light coming from above, where it bounces off. Same with many bacteria, where they look pigmented on a petridish but colorless under the microscope.
@@MicrobehunterMicroscopy It also depends upon conducting a proper blood smear with the edge of a second slide, which gives the effect of a single layer of cells smeared across the slide as opposed to an incorrect smear which pushes the cells together stacking them giving them a reddish color but making them useless for counts or recording any usable observations.
Will it work for neurodegenerative
Plz make a video why we use methne blue to see cheek cells, and why we use safron to see onion cells plz 🙏 tell the reason
How can I stain the nucleus of protozoans keeping them alive?
Try MB on covid 19 ✔️
I fear of needle..😭😭😭😭...for blood under microscope mag did u use sir 400x?
I can't find mounting mediums anywhere in france or on online shops or any stains anyone knows where can I get those?
try brunel microscopes in the uk: www.brunelmicroscopes.co.uk/
Hi haji , methelene blue , is available aquarium fish pet shops , it is used by fish keepers to treat sick fish .
He is so happy about his teeth bacteria 😂
Is 40x magnifying is enough for lookinh cell ?
no; 100 is better
try out a simple stain with methylene blue
Can I use food colour instead of methylene blue??
Plz reply...
There are so many different kinds of food coloring around and it depends to what other substances they stain. The best thing is to try out.
I have a question. I took my dog to a gentleman to see if my dog was getting close to her heat cycle. He surprised me when he pulled out the microscope 🔬. He had 3 dyes. He dipped the slide after he collected her blood 🩸 into 3 different types of dyes . He put it under the microscope and was looking at her cells . We ended up breeding her and she took . Where can I get those dyes? It was a light blue on a blue one and a red one ? U know what they are called or where can I purchase them?
Look for Diff-Quik or Rapi-Diff staining
@@Nixtev thank you so much. I’m going to look at some now.
Why doesn’t the methylene blue cause lysis of the blood cells? I have a diluted (aqueous) solution of methylene blue and want to try a blood smear
What Magnification were you you sing
Sir plss use safranin next time and ur videos are very good
Hello
Where to buy it in France ?
Chemical supplies shops have it, but if they do not sell to private people, then try to buy from an aquarium shop. Methylene blue is also used as anti-fungus treatment for aquarium fish.
@@MicrobehunterMicroscopy But where to buy the same concentration as in your video?
Do it with yeast
Do I require a fluorescence microscope to view cells stained by Methylene blue stain
No. methylene blue strains directly. No fluorescence here.
Can we see it on an animal cell?....great vid
now we have DIC and staining is not nessecary
Plz koi hindi me ye video banao
Sahi baat Bhai