I put my DNA under the microscope and you can too đŹ Easy DNA extraction
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- Äas pĆidĂĄn 29. 04. 2020
- I show you how to extract DNA and how to put it under the microscope. Individual DNA molecules are actually too thin to be seen under the microscope. The fibers that I found are probably many DNA molecules stuck together with proteins and other cell material sticking to it.
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#microbehunter #amateurmicroscopy #microscope - VÄda a technologie
This is the type of Science I wanted to learn in school
You have to take genetic engineering as a career
Me too, now im grade 10 we are learning about gender 127
Exactly you are right I am in class 11 now
â@Scott Pickering how is this "basic" science? because he made it seem easy using common materials?
â@Scott Pickering
â @Scott Pickering
in what reality? hopefully the one where actual scientific thinking applies
what "basic science" do you figure out what is or is not dna in a pool of stuff? how do you know what breaks down proteins and cells to get at dna? how do you know how to separate dna from other things? can you explain the "basic" science to get at all of the information being presented for each step?
So, since its "common materials" it's "basic"? regardless of how many non-"basic" sciences use common materials in a lot of their processes.
"its looking at stuff through a microscope".... this is like saying "its looking at stuff using a calculator/computer" for rocket science....
If you just "looked at stuff through a microscope", would you know what you are looking at at all?
Keep in mind how he can't verify that these things are indeed dna or not, or if the clumping is from protein or not; he's making educated guesses based on "basic" science?
Do you think "astrophysics or rocket science" can't have small inaccurate experiments like this that make things seem "basic" because it's not a "basic science"?
How exactly do you define "basic" science, because I don't see a consistent approach being used here.
It's likely an aggregate of supercoiled DNA, the 300nm loops I think would still be way too small. And if you recently ate something especially with starches, you might be seeing a lot of alpha-amylase proteins! We did some similar things in my Biotechnology lab.
I think this a great activity btw, it's a really good basic example of DNA extraction without needing purification columns and lab grade detergents
It could be condensed chromatin fibers, which is roughly 700 nm wide. That could be seen with a light microscope I think.
why do we see alpha-amylase proteins after eating starch?
@@tannukashyap8311 Looking back on this you wouldn't be able to actually see the proteins like that, too small. I think I was assuming there is an increase in alpha amylase enzyme production in your mouth during starch consumption to help increase digestion, but I don't actually know if that's true
@@SystemUpdate310 I think it's possible to see 700nm in a light, and yes it is condensed chromatin fibers, heterochromatin is 700
@@tannukashyap8311 they are enzymes used in the digestion of starch
So if you eat a lot of starchy food, then your cells (Intestinal for this one in particular) would respond by synthesizing more alpha amylase enzymes
To help in the digestion of it
Amazing!!! I wish more people got interesested in content like this!!!!! Just fascinating, thank you very much
Totally magnificent dear
Exactly :)) people should show their more interest in science ...
Najma Samantha...Microscopy, and amateur astronomy are the best hobbies anyone can get into!..It sure beats coin, and stamp collecting which I find to be dull, and boring!
@@damontsekos365 hobbies are subjective. I find stamp and coin collecting boring as well but you canât go around telling people what they should be more interested in
People are suprised that Iâm 11 and I like science and not stupid video games
Such a genuine content .
Not the cringe as other creator
I wish this kind of proffesor are also present in pur collegeđžđž
Hai Apoorva were is our college in India
Academia has an agenda ,,,keep them dumb ,,,,not too dumb.Evil to the lying core.
Man, it's awesome. Keep up the good work đđ»
I am a young and new passionate science teacher! Glad I found your channel! I love learning the content you produce Sir
Amazing as always. Thank you for all you do. This is my favorite video I have seen.
I am sure you can do fluorescence microscopy . You could use a DNA fluorochrome stain such as Acridine Orange to stain the DNA. Alternatives are natural DNA staining fluorochromes include Berberine (from Barberry - Berberis spp.), and extracts from Greater Celandine (Chelidonium majus) which is a common plant in many parts of the world, and Plume Poppy (German = Federmohn) (Macleaya sp). You don't specifically need UV for this fluorescence, because most of these stains can be excited with Blue light and emit a yellow gold color.
The open source licensing s very honorable. Thank you very much!
Absolutely!! 100%
woah. Thatâs cool. Canât wait for more content!
Most excellent spit sample microscopy analysis. Whether or not we could see dna is compensated by impressive discussion of observed micro forms and structures. Bravo!
O my God! that was such an amazing experiment oliver.....Thank u so much..
Wow excellently cool experiment
Great video again
Loved it!!
You are terrific! I love your presentation and the information you bring us. Two thumbs up!đ đ
Im learning a lot more with your videos than with my classes at the university. I've seen your gallery, it's fantastic, I use the pictures to compare what Im looking in the microscope. Your job is amazing
point of classes at university is to give you the background to allow you to get to these types of things on your own, designing and figuring things out.
just watching and learning specific things is mostly only beneficial for a particular job or task. it can be more motivating because its more interesting, but it should motivate you to learn the class stuff.
Then you must not be paying attention in your university classes...
Awesome experiment sir đ.Thank you for sharing.
Sir in a very beautiful way you have shown DNA. It is really fascinating.
Bro it's awesome and you are a good content creator
I love your good vibes. TY for the teachings. Much love.
Wow đ€© I like the colourful microscopic background stunning đ thank you for sharing
You have the best channel on microscopy, but you need more videos about vintage microscopes, vintage (and modern) staining methods, and diy staining (there's something on the internet, but I only believe in your recipes đ).
How cool! Thank you for sharing!
I just downloaded a PDF on doing the banana activity. Going to do it with my grandchildren. It was great to see how all was done, Thanks
Thanks for the upload...
Just discovered the channel and subscribed. Very nice content!
wow i was just asking myself where would i be able to find library of these mico friends .Thanks alot!
me too mate.
The video was interesting keep it up good work!
Great day for me to see DNA live. Thank you.
You are an amazing person,
This is my first video of your
Thank you for creating this image database!
I had to do so much scrolling to find this video! Thank you!
I really enjoy seeing microbes. I am glad to have found your channel. Thank you!
Sir you could use lens cleaner as protease to degrade proteins (histone) that binds up dna and see in micro scope of high resolution you may get a better view of dna
I tried it and it worked!...Thanks!
Great video!
It has a beads on string structure so probably is chromatin material.
yeah i agree
Nice info . Thanks for this video.
you should've used eye drops as they have cellulase and amylase and starch would be destroyed, more chances of actually seeing DNA. although i think the micrographs you got at the end, the little beads on string are actually DNA. you madman Oliver I love you you did it
Thank you so much for doing this video. I found it exceptionally engrossing. I appreciate learning how scientists make the "DNA" strands they can take out of the tube. I've always wondered about that. Bravo!! đ
I've done too at my school! Although the result was different from yours , but it made me really excitedđ
Brilliant educational content.
It was a very interesting project
Great video đ
I don't have my microscope yet. It will be here Wednesday. But I still find y our videos fascinating. I had no idea one could isolate DNA like this. I guess I better start saving up for my very own Scanning Electron Microscope. :)
Jesus, SEMS are very specialised and expensive! Based on many factors and the functionality I would so prefer a expensive compound microscope. But I guess its down to opinion, I hope you will be satisfied đ Aplogies to my terrible communication, I am much more used to typing on a computer then typing over a compact electronic device like a phone.
Thank you so much sir for this great work.I was really excited to see DNA . Love from INDIAđźđł â€ïžđźđł
Incredible video! Seeing any amount of DNA under a light microscope of all things is far better than nothing at all. Thank you for the tip.
However, can you go more into detail about this procedure? Never heard of this before and not too sure about the materials, for example if it is better to use 99% isopropyl alcohol or something much lower like 70%.
Many Thanks again!
I have used 96% (about) ethyl alcohol. It works better when the alcohol is ice cold, but I did not cool it and it also worked. The DNA participation with alcohol is standard procedure in molecular biology. The alcohol reduces the polarity of the water to the extent that DNA can not be dissolved anymore. Normally one would collect the DNA by centrifugation after the addition of alcohol.
This is f âing awesome holly crap
Superb this is awesome
This is the real science .. Love it
The laugh after âwood is goodâ XD i actually died
I love your channel and hope to donate soon!
Thank you so much!
I hope....I can find a teacher like youđ
I had experienced and did many experiments in the high equipped lab while doing my post graduation in microbiology discipline... !
My researches included microbiology, biotechnology, biochemistry,cell biology and molecular biology... !
So you would say it is DNA that we are seeing?
I study b SC biotechnology 1st year. In my practical we do Isolation of DNA from both plant and animal tissue. it's gave a best results.
The sound when he spit out salty water was satisfying đđđđđ
Nice work!! What happens if you rhythmically press on the cover slip for a while? Does that break up the clumps of DNA?
Amazing try buddy thank you so much so real
i heart you, microbehunter.
i did it mr. oliver kim thk u
helped on my project
Subscribedđđ
This is what I wanted to see đđđđ
Thank you very much. I learnt a lot.
Alwaysss Support â€ïž
Sir plz show some solid elements powder or stuff like that in a microscope..
i've never done more than school microscopy, but i wondered if just putting like an piece of plant or insect on a glass was enough to see inside and since you said something with dish soap, that breaks the structure and makes the cells inside visible, is this the way to make the inside visible? or is the inside visible anyway? i've read thing about microscopic knife too...
GOOD LECTURE đ GOD BLESS YOU TEACHER đ
Love this video
I know that the DNA, which is an acronym for De-oxy-ribo-Nucleic-Acid, is a double helix structure with some tiny steps and phosphate sugars on it but, I don't think I can actually see those things under my personal microscope. What is the name of the detergent you made use of? Is it Persil, Tide or Gain?? Or did you make use of any common detergent? I'll try this experiment once again and make sure I get the right viewing under the microscope. BTW, this video was amazing! Thanks for sharing.đđđđđ
Wow đČ , thank you sir đâš
đ I really like the Microworld Archive idea! Which microscope and camera are you aiming for?
How much does the microscope cost and do you have a link for its website?
Thankyou so much đđż
I still wonder if my science teachers were this amazing ..I would have choosen science for rest of my life...
Though with no regrets ... Being a commerce graduate...I still carry my interest for science experiments. đ
Not too late my friend, sit down, make plans and go for it, live while you are alive.
Oooo i can believe that there are so genius CZcamsrs like u ....this what i like to see ...i subscribed u i liked u..thank u sir
Hi, this is really interesting. What if we try and see bacteria in that solution? Would this be possible?
You are very humble
Well done
Wow how smart... Is the rounds things cells
Can you make a video of mushroom cells under a microscope?
it would be great if you could include the magnification and ledger in the photos. very entertaining. TY
Amazing maannn
Very interesting.
Thank you.
With good education everyone can be a doctor or related field.
Microbehunter you may use pipette, syringe, medicine dropper either đ .
Nice video.
Thatâs so cool
I love the moment you pull your saliva from the measuring cup and call it a clump of DNA :D
Nice video
Good one! Great topic. What kind of microscope are you fundraising for? I want to read the specs. :)
It's an Olympus BX53 with Planfluorite objectives (4x 10x 20x 40x 100x oil) and Differential Interference Contrast (DIC), which is expensive but produces very nice images. You need a separate prism for each objective and they need to be fit in into a condenser with a rotating turret. I obtained several offers, all similar in the price range, some even more expensive.
@@Microbehunter OoO an Olympus! I hope to be able to donate too!
great sir, excellent!
Nice brođđ»
Wow thatâs so cool
It reminded me of my biology labđđ
The wood also good in case any of the DNA happens to be vampiric.
Ok. Here I see someone teaching microscope. Here I am. An enthusiast đ„ž
Hello, Iâm currently a freshman starting veterinary medicine and Iâve been looking for information on how âfarâ a microscope can let us observe. My question is, and I would appreciate if you help me answer it, could one observe the DNA of an animal thru the microscope? Other than bacteria and cells, what else can a microscope let us observe? Please and thank you.
Thanks sir
Tooo...... Interesting... đ
Why best channels are underrated?!
Amazing â€
What magnification was this? I have a child magnification thing and I'm gonna try using that over winter break cuz this seems super cool
Please make a video on how to match two dna
@Microbehunter , Which microscope were you wanting to buy? Did you buy it yet? What makes it expensive?