🔬 Make Onion mitosis CHROMOSOMES visible - Staining Tutorial | Amateur Microscopy
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- čas přidán 22. 06. 2020
- Here I show you how to use Carmine Acetic Acid to stain the cells of onion root tips to make dividing cells visible. The chromosomes can also be seen.
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Nice one! You will find dividing cells only in the meristem, under the root cap, so the percentage of dividing cells heavily depend on how small tip you cut. Also division is in sync with photo period, best to take the root tip in the morning few hours after light period starts, you will get the most dividing cells, in the evening you will get the least.
Instructive tips 🧅
Expecting more staining methods... I totally satisfied this episode.
can I just use methylene blue?
excellent video showing the technique for staining as well as the mechanical aspects of squeezing the specimen
Really interesting, especially seeing chromosomes and xylem structures like that.
Wonderful!!! Will try this week!
I loved it.....Thank you Oliver....
Wrap the clear glass in something opaque like foil and place the onion-cup in the light. The more photosynthesis that is happening the more dividing cells will be found in the roots.
hi, I'm doing this project for biology, can you explain what you mean? Do I put the onion covered with foil in the light or already stained root in the light?
What I meant was to cover the cup the onion is setting in with foil in order to block light from hitting the roots. But you do want to expose the onion top to light to initiate photosynthesis. The photosynthesis will ramp up the cell division o the roots.
If you stain the root it will no longer grow so don't stain until finished.
@@leftblank3482 Thank you!
Hello Oliver, thank you for answering my question :)
I'll put my mitosis photos on the website you created xD
thank you!
I have tried this with toluidine blue stain.....The results are same but the nucleus is stained blue rather than red......
Hi, your presentation is really nice. I have a small question. Can we use Indigo Carmine (E132) instead of E120?
Is methylene blue a good alternative stain? It's the only stain I have.
you are the best microscopist ever! you made me want to becoe a scientist
I know. He's making me want to be one too.
Thanks for the video. TBH, this protocol is a pain in the backside, as it requires quite some skill (which is true for most cytological tasks). For my species (Master thesis), it just didn't work until now. Do you have experience in making permanent slides from these?
I have ready made permanent ones so I don't have to go through all that trouble in preparing them myself, and mine are animal cell mitosis, but they look the same as the onion ones!
Sir, could you please mention the magnification on the screen🙏🙏
amazingling taught...
with high school biology
Please answer me, can i replace the acetocarmine by neutral red? I have not the acetocarmine stain in my laboratory
Wow it's awesome. which microscope? Even I want to see it
how long is the staining solution stable?
Sir Please tell us that what stain we used if we have not acetocarmine .
Your video 🫡🙏🙏
How can I make permanent slides of this?
Well done! Thank you and I subbed ya just now.
4:40 - are the round things chloroplasts?
Can you show us the DNA in the chromosomes? Or tell us where we can go to online to see them?
Comment préparer le carmin acétique ?
Les étapes en détail svp?!
you should always tell us the magnification you view under
thanx
why do other people always fixate the roots before staining?
Which microscope
Can I see the chromosomes of other plants by this method ?
Hi Sir. Can you help me with how to prepare microscope slide of an earthworm? Thank you.
That's more complicated. You would have to microtome it. Or use a stereo microscope to look at the whole worm (recommended)
Make other videos which involve staining
Nice video., I saw the 16 chromosomes which are supposed to be.
Can i use methylene blue???
I use methylene blue with great success. But I also pretreat with 1M HCL gently warmed over a flame for about a minute. Then rinse HCL off and add stain. Carefully Squash with coverslip in place. try to remove excess stain by adding water to edge of coverslip and wicking it across the specimen by placing a tissue on the opposite side of the coverslip. and enjoy! Great fun!
Thanks for you suggestions
My methylene blue won't stain bacteria for some reason could you make a video
Please
Could it be that you need a more specific stain. Methylene blue is excellent for chromosomes, not bacteria.
@@Rick-tt6yq thank you
+Colonel Doggo...Use crystal violet or eosin!
Can i use tea to make it visible?
Try it out, but make sure that it is very concentrated. Otherwise you might not see it.
@@MicrobehunterMicroscopy I will try.
@@MicrobehunterMicroscopy not green.... Not white... Not red...
Puerr....
You don't use hydrocloric acid?
Maybe HCl would also work to break down (hydrolyze) the cellulose in the cell wall. I guess one could try. The "official" protocol states that one should use acetic acid. Maybe it also works with vinegar (4% acetic acid) and then one might have to heat it longer.
HCl is not necessary for all genera. Additional hydrolysis at 60°C can be done prior to staining, but it really depends on the taxon. In Onions, it's not necessary. In brassicaceans, e.g. Cardamine spp., it can be helpful
I use 1M HCL, gently heated for about 1 minute. Then flush with water, stain, squash and enjoy. I don’t split the root tip either but I like that approach and will try it next.
Чи можна замінити кармін?
E coil bacteria under microscope
Sir plz hindi language
And please reply. I am very sad