HPLC Troubleshooting-Split Peaks | hindi

HPLC Troubleshooting-Split Peaks | hindi
your quires;
this video based HPLC splitting problem and also explained the causes and solutions
HPLC
peak
split peak
peak splitting
Split peaks can show up in gas chromatography when our injection isn’t working right or things aren’t transferring from the inlet to the column correctly. When troubleshooting split peaks, we should pay attention to our inlet configuration and setup, and we should confirm that everything with the injection is working correctly. We also want to make sure we’ve chosen a column stationary phase and solvent that are compatible with one another.
HPLC troubleshooting
voice of kayani
chromatography
HPLC injection solvent
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[@muhammadarshadmirani9764]

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[@saranoor1981]

Sir peak height ku nhi ati. Kuch injection sahi chaly then peak height and integration bhi nhi ho rhi

[@voiceofkayani5419]

Sample preparation issue ho sakta hai ya injection volume ka ka problem hai

[@saranoor1981]

Right sir

[@saranoor1981]

Height buhut choti hogae k integrate bhi nhi ho rhi

[@voiceofkayani5419]

Integration ho jati hai option hota hai software Mai detection ka wo Kam karnai se small peak bhi detect ho jati hai

[@saranoor1981]

Sir pehly large peak a rhi thi ab peak small a rho hy same concentration hy injection volume bhi same hy then achanak peak small hogae and area bhi show.nhi ho rhi

[@voiceofkayani5419]

@@saranoor1981 it shows k sample mixing theek nai hai