Top 10 Most Common HPLC Issues and How to Fix Them (2023)

Welcome to my comprehensive guide on the "Top 10 Most Common HPLC Issues and How to Fix Them" for 2023! If you're a lab technician, researcher, or anyone who frequently uses High-Performance Liquid Chromatography (HPLC), you know that issues can arise. This video aims to tackle the most common problems you might encounter and provide easy-to-follow solutions.
Chapters:
0:00 | 1) Baseline Noise
1:10 | 2) Ghost Peaks
1:51 | 3) Peak Tailing
2:33 | 4) Peak Fronting
2:53 | 5) High Pressure
3:27 | 6) Retention Time Shifting
4:15 | 7) Loss of Resolution
4:30 | 8) Split Peaks
5:18 | 9) Loss of Sensitivity
6:13 | 10) Rising Baseline
⬇️ Get Affordable and Dope Lab Consumables Here ⬇️
(No pun intended, unless you're a cannabis lab, then pun intended)
filtrous.com/
Want to Connect with Me? ⬇️
LinkedIn: / david-yazdi-a048519

[@rpsly1873]

Very informative, Great video !!!

[@patrickgassenbauer7070]

Helpful and to the point! GJ

[@aladindz_ch]

Thank you so much sir, it was so helpful.

[@moulayemhamed154]

sir, I have a problem making the necessary dilutions for the calibration curve. I also have a problem in the anion pump which displays “CMB-20 error” I cannot find the solution. finally I work on an ion chromatography with double pump and automatic injection. I thank you for your help.

[@geekyogurt1904]

For issues like ghost peaks, is there a way to run a no injection instrument blank (run just the gradient program with no sample) on an LC system? I've done it on GC to rule out sample prep or dirty injection needles

[@user-sc6jv1bj7r]

Shimadzu LC2030 automatic sampling system fault repair how to repair😊

[@younesdahmani715]

I need your email sir

[@DavidYazdi1]

What's going on friend?

[@younesdahmani715]

@@DavidYazdi1 I need your help sir how can I calculate (Purity Angle and threshold Angle)
in labsolution shimadzu HPLC-PDA

[@DavidYazdi1]

Open the Software: Launch LabSolutions and open the data file containing your chromatogram and spectral data.
Navigate to PDA Data: Go to the section where PDA data can be analyzed.
Select Peak: Choose the peak for which you want to calculate the Purity and Threshold Angles.
Run Analysis: There should be an option to calculate or display the Purity and Threshold Angles. Follow the on-screen instructions.
Interpret Results: Once the angles are calculated, compare them to assess the purity of the peak.

[@mavicdelapena1545]

Agilent HPLC no peak problem- how to solve?