# 1 Most Common Problem in HPLC: Air Bubble in the Pump

The most common issue encountered in High-Performance Liquid Chromatography (HPLC) - an air bubble in the pump. Discover expert tips and techniques from Axion Labs to quickly and effectively resolve this problem.
Key Highlights:
Identifying Air Bubbles: Learn to recognize the symptoms of an air bubble in your HPLC pump, such as unusual pressure ripple and fluctuating UV signals​​.
Simple Fixing Method: We demonstrate a straightforward procedure to eliminate air bubbles, including opening the purge valve and adjusting the flow rate​​.
Verifying Solution Effectiveness: Understand how to check if the air bubble has been successfully removed by observing the pressure ripple and ensuring it falls within the acceptable range​​.
Software Tips: Gain insights into using diagnostic tools in HPLC software to monitor and confirm the pressure ripple status​​.
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[@bpopa]

Love the pro tip of flushing the column completely by almost closing the valve all the way.

[@365freekill]

I followed your tip and it worked. Thank you so much!

[@israrahmadsani4595]

Kindly make a video on which properties of molecules require to develop acurate method development .. ( properties of molecules/drug vs which conditions or parameters should be select)

[@israrahmadsani4595]

Sir I want to develop a method for pelargonium sodiodes on hplc I tried methods available in research papers but that's are not working