HPLC Chromatography Demonstration

Hi Friends 👋 😃,
Join me on Telegram - t.me/baayo_official
In this video I have done a demonstration of #HPLC Machine in collaboration with 'Spinco Biotech' company. I have first talked about the basic concepts of HPLC and its principle. Then I have demonstrated the main components of an HPLC machine and the steps involved in HPLC process, so you can relate to see How it works in real? There have been many advancements with latest HPLC machines, you will also see how we can operate HPLC experiments just with our fingertips using mobile application now. Hope this video will be useful to all students and professionals who want to learn about HPLC and see it in action.
More about this HPLC Machine here 👉 -www.shimadzu.com/an/products/...
More about Spinco Biotech here 👉 - spincotech.com/
Instagram - / baayo_official
LinkedIn - / artirani
If you just want to learn the theoretical concepts of HPLC, I have done one other video in the past, you can watch "HPLC Chromatography Basics Explained" here 👉 • HPLC Chromatography Ba...
If you have any doubts, Please leave a comment in the comments section below. 😀
Please SHARE with all your friends , SUPPORT and SUBSCRIBE to this channel ! 😀🙏🏻
👉 Subscribe: / @baayo
Other Chromatography Videos:
=========================
HPLC Chromatography 👉 • HPLC Chromatography Ba...
UV Visible Spectroscopy 👉 • UV Visible Spectroscop...
Gas Chromatography: 👉 • Gas Chromatography Pri...
#hplc #chromatography #baayo

[@BaaYo]

To access notes and practice questions, please join our Telegram channel t.me/baayo_official

[@DeepakVarma-bv3dz]

Yes

[@rajchauhan5634]

Detector ke vdo bhi bnao

[@sanikahasbe]

This video is very helpful to students because we only learn theoretically like principle, mechanism but we don't know how is HPLC instrument in real which is used in industries.There is huge difference between learning theoretical and watch the practically because while we learn theoretical only we can imagine the instrument but when we watch the video we know the how is instrument,its working.Our human brain has power to grasp pictures quickly so this video is very useful to the students because of you gave excellent explanation like instrumentation, working, components of HPLC.Mam please make more videos regarding UV,IR,Mass spectroscopy.

[@BaaYo]

thank you @saikahasbe means alot. Yes sure i will come up with all the instrumental videos

[@shreyagarg634]

Thanks a lot mma

[@reshmadeshmukh253]

True

[@hollywoodmovies129]

I have a question if in cases a new column is used then peaks areas are different from previous peak areas. What kinds of troubleshooting in the HPLC system. How to calculate RSD 2%, system sustainable

[@vineettiwari5473]

As Working professional in industry,the things you explained in 10 mins. I learnt it in one Month.
You're are super accurate.
Just put labsolution integration detailed video. I would greatly appreciate it.❤

[@1to1000funnyvideos]

outstanding explanation 👌pdhne se phle bahut hard lag rha tha but after watching yur video ye topic bda hi easy lga

[@comewithme3660]

Thank you ma'am for the amazing demonstration... It's going to be very helpful for me. I'll save this for later.. looking forward to such an amazing demonstration.

[@rachunagalikar7193]

I saw your videos when I was studying B.Pharma (2018), I passed the exam (Pharmaceutical analysis) by watching your videos only.
Thanks a lot.
Love from Karnataka❤

[@BaaYo]

Thank you 😊

[@insiderweb.]

Very helpful for my job interview Thanks Mam 😊

[@kartikeyverma6651]

Nice initiative....try to make more videos like that on Gas chromatography, Mass Spectroscopy, MS-MS, GC-MS , HPLC-MS etc. It is quite helpful...I remember the day when I saw HPLC in CSIR lab (it was one day visit to CSIR lab). One scientist there not only explained the principle but also showed us the working of HPLC, GC-MS and MS-MS and to be honest, on that day I understand the actual concept.

[@BaaYo]

Thanks!! Great to hear you got an opportunity to work with scientists and got practical lab experience. I am trying to bring the similar experience to people online so it gives a better visual understanding to everyone. Will definitely plan to cover other topics as well. 👍🏼

[@abhijeetrathore8219]

Thank you ma'am this video was very helpful for me as well i have have shared this video along with my batchmates they found it useful too in terms of getting practical knowledge about such instruments. Keep sharing practical videos ma'am with some real samples and results as you did. Ma'am i am looking for intership or training in such labs to get a practical exposure and handling such instruments if you have any oppourtunity then please share with us ma'am. Thanks alot ma'am even your lecture videos are also useful during revision of technical knowledge. @@BaaYo

[@sonaligupta-tg5zg]

Very nice. Deeply understandable.

[@saikrishnasurajbavirisetty7804]

Mam please do videos on other instrumental analysis too, these are very helpful, appreciate your work👏👏

[@shreyanshmishra968]

Your amazing explanation 👏

[@kosatish]

"An outstanding demonstration of the #HPLC Machine! The comprehensive breakdown of HPLC's fundamental concepts, principles, and operational steps provides with a clear understanding of its functionality. Witnessing the integration of modern technology, like the mobile application for experiment control, showcases the remarkable advancements in HPLC. This video is an invaluable asset for students and professionals alike, offering a profound learning opportunity about HPLC in action. Kudos for sharing your expertise and collaborating to make this informative content possible!"

[@shankarpawar412]

Informative ❤

[@xyzchemistry2023]

Great Explanation

[@javednalband6155]

Thank you so much mam for making this video ❤❤❤❤❤❤

[@agricultureaswintamil6669]

I’m HPLC RESEARCH DEVELOPMENT ANALYST VERY GOOD explanation 😊

[@BaaYo]

Thank you.. please share this video

[@md.abdurrahmanrahad]

Very helpfully for us.

[@HamidAli-Rph]

I love your method of communication is so wonderfull.
I watch your each and every lecture which is very helpfull

[@BaaYo]

Thank you

[@girishchandra5568]

Very helpful,, thank you so much ma'am 🙏 UHPLC shimadzu N series ka bhi demo bata dijye plz,🙏🙏

[@anjaligorai6943]

Thank you, ma'am. Really it's an interesting, informative & valuable video demonstration.

[@BaaYo]

Thank you Anjali ❤

[@Instagram12141]

Wow.....this video is helpful 😮😮😢😢

[@bunnyvivek8183]

Very Well explained❤❤

[@udayakumartalwar568]

You are great arathi ji

[@chetantake1105]

Good evening mam
Nice information and nice instrument.
May u convey me the software used to operate.
And one more, the peaks which u demonstrated it may be contamination but it is well resolved either increasing oven temperature or increase in run time.

[@yousufalimazumder8350]

Need more vedios about HPLC operation

[@shahistabegum2008]

Trust me this is one of the phenomenal vedio on hplc thank you so much Ma'am

[@BaaYo]

Thanks a ton

[@mansoorali8256]

Ma'am please upload a video on HPLC-DAD the working principle and instrumentation

[@lo-fi_huntsmaniax2691]

U r the best!!

[@AdityaRaj-zk9eh]

thanks mam

[@verygoodexpandedpande1407]

Thanks mam..nicely explained 🙌🏻🙏♥️♥️

[@BaaYo]

Thank you.. please share with others

[@vrajpatel5561]

Plz upload HPLC and GC Calibration videos also

[@NaveedAhmed-zn7wd]

Extreme thanks I am very much excited and want to see the energetic in the same way.....
Plz madam make video on all techniques... NMR... Mass spectrometry....
With clear instrumentations.

[@BaaYo]

Sure I will make.. thank you 😊

[@murthy2108]

Excellent, Nice & Very Informative

[@BaaYo]

Thank you so much

[@kanishjudo88]

Arti mam how samples can be detect through HPLC in same time and what is PDA detector

[@supreetsingh6709]

Very nice! what is system suitability if you can explain?

[@jeetcollection]

This is an insightful and knowledgeable video, ma'am. I am preparing for my interview(M.Pharm) and I came across your video to know the basics to recharge my brain and this video helped me more than I expected.

[@BaaYo]

wow thank you.. best wishes for your interview 😊

[@Mamtakumari-tf3ep]

Wooow maam 😊😊😍😍😍 thank you for sharing with us 🙏🙏❤️♥️

[@BaaYo]

Thank you Mamta ❤

[@vershachauhan775]

Thank you so much ma'am for this video

[@BaaYo]

Thank you too

[@muskaandhingra5163]

thank you

[@pradumkushwaha2733]

Very nice vedio

[@avijitmaity1649]

Can you also please tell about how to take out the fraction of sample from hplc? * the fraction whice gives peak in chromatogram...!!

[@rupa_handle]

Can you provide a video of bpa detection method for shimadzu HPLC machine???

[@anchudhary5122]

Thank u so much ma'am for this information

[@BaaYo]

Thank you

[@educationpoint778]

I Have watched many videos . but this one is too much simple and informative. i need to know how to studys the results of samples with details, if you can give me lectures in individuals i can pay you also for lectures, but i need complete concept to learn the LC operation,, i am studying in China PhD student

[@shampamandal3336]

Plz mam continue it... It will be very much helpful

[@BaaYo]

yes i will continue making these types of videos

[@suryasmanmohanty9000]

How to separate the other element other than the first element which removed out from the machine, which is adsorbed by the silica..?

[@kajalagrawal02]

Superb ma'am, thank you soo much👏🏻😍😍

[@BaaYo]

Yeeyy thanks Kajal ❤ hope u liked it

[@kajalagrawal02]

@@BaaYo very much mam😍

[@mangaleshwarprasad7936]

Maam aap bahut hi ache tarah se explain karti hai

[@BaaYo]

Thank you :)

[@rajchauhan5634]

Detector ke vdo bhi bnao

[@raktimroy3797]

Much needed❤❤❤ ICP ,xrf ke upar vi aysa banaye

[@BaaYo]

Noted

[@RakeshKumar-br4tl]

Very informative vid.ma'am instrumentation series is nice initiative ma'am 👌🏻

[@BaaYo]

Glad you liked it

[@krimislifelines3234]

Thank you so much 🙏🙂😃😘😀

[@BaaYo]

Thank you too for watching and liking

[@Aditya-020]

Thank you so much mam 😊

[@BaaYo]

Please share

[@user-qf9uy6tf6b]

Nice

[@vijaykargirwar6950]

I am working in thalassemia unit Mumbai.

[@life.moon_to_sun]

Please tell about integration in hplc

[@prajwalnalage7081]

Thank u for information

[@BaaYo]

Any time

[@santoshchauhan-pk6ly]

Thank you soo much

[@BaaYo]

You're welcome!

[@jitubhatt69]

Pl.give information about ftnir

[@shaikhnishatbanu2693]

Outstanding 😊😊

[@BaaYo]

Many many thanks

[@RaviKant-tb4vh]

Or other instruments per bnayo,,like Gac,UV,Mass,NMR,and other

[@vershachauhan775]

Ma'am please aise or videos bnaiye hptlc or baki molecular techniques pr ...

[@BaaYo]

Yes cover krege :)

[@Shabbir2749]

I have seen only one CZcams channel which conceptually explains different biological techniques. Please keep it up.

[@BaaYo]

Thank you, I will

[@samiiiayyub6913]

Please explain flash chromatography

[@shekharnalawade7249]

Thank you so much mam

[@BaaYo]

Thank you too

[@rushikeshbhosale7284]

Thank you Mam 🙏

[@BaaYo]

Thank you too

[@dikeshathakur6141]

Give demostration on reading chromatogram

[@AnusShah-yl9wd]

Love from Pakistan❤

[@lcmotivationlifecare746]

Thank you🌹🙏

[@BaaYo]

Please share with others.. thank u

[@lcmotivationlifecare746]

👍👍👍

[@shubhamkumar-hu7ts]

Nice ma'am, thankyou!
Please one video for how to give commands on software

[@BaaYo]

Noted.. I will make one video for this

[@user-ku5jz2qg3m]

Mam ek video operate karke bhi batana Or Isme U.V.detector bhi hota hai wo kha hai

[@ratheradil3025]

Thanks a lot mam. Please make a video on DNA damage and repair if possible.

[@BaaYo]

Okay sure

[@ratheradil3025]

@@BaaYo Thank U

[@DishantPatkar]

Thank you mam 😊

[@BaaYo]

Keep watching. and thank you too

[@prashantghongade7521]

Please demonstrate other HPLC instrument like waters, Agilent and GC instrument also

[@BaaYo]

Sure 👍🏼, planning to create more such videos.

[@hanmandlumaradi9795]

What about paracetomal analysis in hplc.?

[@pad480]

Please make more videos HPLC Demonstrations
Software handling new method create

[@BaaYo]

Sure I will

[@balagaravi1255]

hello how could we detect the leakage in the intrument

[@afreenakhtar457]

Thank u ❤

[@BaaYo]

Please share with your batchmates

[@pmeet4654]

Bhat din baad apaka video aya mam ap regular video banaye

[@BaaYo]

Yes regular rhegi ab.. please share with ur colleagues

[@priyanshabhatia209]

How to determine buffer according to drug polarity?

[@BalwantSingh-qk8yc]

mam gc,glc kai bare mai or hplc mai pdne kai lye koi bdiya pdf yaa notes mil skte hai brifly

[@blogger.powerpoint_expert]

How to interpret that chromatogram?

[@vijaykargirwar6950]

HPLC in thalassemia. Pl guide

[@surendrapardhi2719]

Very nice

[@BaaYo]

Thanks

[@safaltakikunji4075]

Ma'am जी, हम कैसे identify karenge ki collection me kon kon sa component है

[@GoluKumar-uv2tp]

How to make bufffer solution in hplc

[@harshilrana1414]

I am not able to find 34660 area can anyone please help where it is located

[@user-mw2ez6np2f]

Mam ❤

[@Monkeylluffy]

👌👌👍

[@BaaYo]

❤️

[@OfficialMr_nits]

👌🙏

[@BaaYo]

🙏🙏

[@dipyamanbagchi3848]

Mam how to calcute dilution studies

[@DineshLimbu-dh1js]

Is this a same hplc instrument that is used in pharmaceutical industries ?

[@BaaYo]

Yes

[@satyamsharma1937]

Mam peak ke area se identify krte honge na konsa element elute out hua hai

[@BaaYo]

yes. Area se dekhte hai..

[@satyamsharma1937]

@@BaaYo ok thanks mam

[@doitlikedaisy2046]

Name of the software?

[@shikaparusharam2477]

Hi madam plz tell empower audit trail

[@VaibhavPatel-zq9ui]

Mam muje sequence banana or integration sikhao
Me pharma me job kar raha hu lekin ye sab nahi aata jisaki vajah se hplc system nahi laga pa raha

[@mangaleshwarprasad7936]

Maam iit jam ke liye koun sa book le

[@parashsharma77]

❤❤❤❤❤

[@BaaYo]

❤❤

[@user-pk2em8lj7e]

G c section one video

[@Mahakal_status_4613]

Understanding Language hai