HPLC Chromatography Basics Explained
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- Äas pĆidĂĄn 2. 06. 2018
- (You can also watch new HPLC video with demonstration here - đ âą HPLC Chromatography De... )
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All you need to know about the HPLC Chromatography!
HPLC(High-performance liquid chromatography) also referred as high-pressure liquid chromatography, is a technique used in analytical labs to separate, identify, and quantify each component in a mixture.HPLC separates and purifies compounds according to their polarity.
I have tried to simplify the whole process of HPLC Chromatography and explain in simple terms. Letâs look at the main components involved in HPLC in this video.
Later in this video, I discussed about Normal Phase Chromatography, Reverse Phase Chromatography and their comparison and differences.
Watch this video for more details.
Other Video Links:
ER TO GOLGI Transport: âą Endoplasmic Reticulum ...
KDEL and KKXX Sequence: âą KDEL and KKXX Signal, ...
If you have any doubts: Please leave a comment in the comments section below.
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thanks a lot for making us aware about the basics of HPLC. Before meeting you, I never thought that a person could be a teacher, support, mentor and philosopher all in one! Thank you for all your hard work and dedication!
I think this is one of best explanation of HPLC on CZcams...
Good lecture mem
Ya
Naa bhai đ
12mins and the whole concept of HPLC and Reverse Phase Chromatography were understood so beautifully â€ïž
This video is a boon for me because I was never able to understand biophysical techniques in class and eventually came across your channel and now everything seems sorted , thank you â€ïž Maâam your teaching style is spectacular đ
honestly no one can teach better than you. How perfectly you explain each n everything. Truly helpful and impressive
I did B.Pharmacy but no body taught me like this during my college years. It was very difficult to understand by reading only theory. You have great knowledge mam and the way you explain is excellent. I am subscribing your channel now. I really appreciate your hard work. Thank you.
Hi even I completed B-Pharm are you still studying?
yes....An lg rha h Man k pass hee bsc MSc Or liya hota ..University prof..srf syllabus complete krne m believe krteđđđ
Agreed đ
@@ranjanadevi7574 how about u? Even m doing masters in pharmaceutics
Heyy m also in my final year of bpharm
Thank You mam.I think I just want to thank you and show my gratitude because your lectures help me a lot to crack my very first interview that I faced after the completion of my post-graduation.Your tutorials are extraordinary to understand.Things would become harder without your videos.Wish your success in every steps of your life â€ïžâ€ïžâ€ïž
Most easiest and valuable information about HPLC on CZcams... respect to you mam đđ»đđ»
Lives simplified by your simple way of explanation . Amazing.
Your lectures are very detailed and informative; you are a very good lecturer...keep up the good work!
Absolutely amazing teaching. Very proper point to point knowledge. So much helpful and accurate. â€ïžâ€ïžâ€ïž
You're the best, you teach exactly what students need to understand unlike the rest of videos available here where they just talk bookish language
I don't find any reason to dislike such an wonderful lecture
Thank you ma'am for explaining in such an amazing way , this clear all my doubts.!! đ
Hello Ma'am! Yesterday, I saw your HPLC video for my job interview. To be honest, I swear on the name of God, for the first time in my life I have properly learned the concept of HPLC through your video. Lots of Love from Pakistan.
I m watching this video again after 3 yrs. And Thanks mam. i wish i could attend ur class in front of uh ..and. i deeply love with ur style of teaching
Thank you very much for your presentation. Would like to know about the buffer you mentioned to equilibrate before the sample run (getting the base line). can you explain more about the equilibration and the buffers to use thank you!
thanks mam,,, i have not comletely understand in 8 yrs in pharma industry and by ur video i just understand in 10 min ..... now i will not forgot this in my whole life....great job..
Thanks a lot Ashwani for kind feedback đ
Very good and nicely explained in a simple manner... I found HPLC difficult, but after watching this my all doubts are cleared đđ
Mene kbhi itni achi explanation nhi li kisi female teachers se u r the 1st one.... Well thanks â€
Excellent & Very Simple Clear & Nice Explanation Madam. Which is Very helped me to Understand clearly. I am Preparing for State Level M.Pharmacy Admission Entrance Exam i am also preparing for GPAT - Graduate Pharmacy Admission Test. Madam thank you very much for Preparation and Upload your Excellent Videos. These are very much useful to B.Pharmacy Students and M.Pharmacy Students who are Preparing Pharmaceutical Analysis Subject (Chemical Analysis).
thank you so much mam for explaining in a good way
Thanku so much ma'am
I just listen you're lectures chromatography & types of chromatography before last day of my exam I gain too much point easily & my concept is clear.
Thanku soooo much
I was very worried about my MSc Bioanalytical Science's , due to online studies i never understood the any chromatography techniques, but today only i found this channel i saw hplc video and now i am not having doubt..i will going to watch more videos, Requesting to please add Bioanalytical related topics..thankx
So simple and much effective explanation... Thank you so much
I am a researcher, your video helped me to understand basics of HPLC. VERY GOOD TEACHING STYLE ! KEEP IT UP.
Thank You :) Happy to help
It was a very well put and precise in concept explanation, otherwise I have to run around searching for separate topics including the mechanism, principle and instrument layout
I have learnt so many things through ur video
I got placed after watching ur videosâ€ïžmy dream has come true. Thanks alotđ„ł
Thank you so much ma'am đđđ it is really beneficial for me & all those students who are looking for good carrier in biochemistry.
As tomorrow is my exam. . . . . But m still watching ur video as my revision. . . . . . Coz u r a best teacher. . . . . Thanks a lot for ur work. . . . . đđđđđ
Am seeing after 2 year... explained easy more than expecting .. its so simple after seeing this video .....đ„đ„
Wow mam ,i was in so much stress like there's so many things to study but now i have so much clearity thank you so much
Thank mam
U are great
I want to know about more vedio
Like kf, gc, HPLC, and their tribal shooting
In industrial use
It was woow lecture simple way n easy. stay blessed mam.
You took my heart..M soooooo happpppy. That I found your videossss...... sooooo Thankful to you. Maam
Simple and precise explanation.Just perfect what needs to be understood about HPLC.Good informative video.
madam, in many cases multiport valve , buffer volume, pulse damper, n guard volumes are used . why?
The what and why for each step explained thoroughly
Thank you for this amazing lecture
Edible.oil.testing.method
Thank you Arti Ma'am,
It's very easy to understand if the teacher taught it in the way they themself understand.
Perfectly explained, I searched this content first time.
Thanks.đđ
Thank You so much Ma'am for such nice explanation..
Tq mam ...tq so much.because of uh i can cover..... my +3 final year DSE-1 papper successfully đlove from Odisha đ
Jai jagannath đ
Thankyou ma'am, you have explained the topic very clearly.. this video has made my concept more clearer... đ
You have amazing Knowledge and great way to transfer this knowledge to others. Thanks , it will help me for M.PHRAMA
Mam you are saviour for us before theory exam đ
Thanks for making simpler I have subject about analysis in Pharm D course
Thank you ma'am .your teaching methods is amazing.l understood only in one seen .no one saw in my uni like you .
Im a big fan of you mam.u r explaining so simply each nd every topic.Ur lectures helping lot me to cover all topics
Thank u so much ma'am đ.. I was searching for this topic from long time and no one explained it in this cool manner . Firstly i thought u will not explain well but no other video explained as good as u â€ïžâ€ïžđ. Thank u so much for your valuable time for us(students)
Thanks Prakash đ
Thank you ma'am Tommorow is my environmental chemistry paper and I am going to write the same .....after watching chromatography videos and this one I feel it's simpler now
Thanks Harleen...and all d best for tomorrow..đ
@@BaaYo mam aik question hai answer kijya ga
How hplc differ form column chromatography
Good explanation.... loved the video... didnât even go back to read the theory stuff...very simplified and well explained... keep up
Thank you ma'am because I got job in ion health care baddi of himachal Pradesh, aap ne itna simple ho kr btaya Qa department m join kiya hun, mere pas thoda sa speak m problem h.... Mein Msc microbiology se 2015 kiya tha.... Thank alot ab mein sar utha kr chaluga thanks alot apka hr video interview prepared krne m bhut help kiya yeh meri pehli job h
Hi mam, I want to quantify triphenylantimony from my sample, for that which mobile phase n which column is needed?
Thank You MAM very nice explanation.
mam u are nirraa ishq teacher what a beautiful explanation of hplc..kudos to your hardwork đŻ
So amazing techniques of teaching
Very simply delivers all concept of HPLC , Thanks đ
Your lectures helped a lot in my studies... Please keep making these videos...đđ
Where are you from
Make Vedio on Isothermal titration calorimetry (ITC) and Differential scanning calorimetry (DSC)
I'm a pathology PG student, thank you so much for this... Even dacie or Tejinder don't have these explanations
Ma'am your teaching style is Unique and very much convincing.. I became die hard fan of your videos.. Please accept my regards, Ma'am..
Thank u ma'am for giving better explanation & plz make a video on diff.types of electrophoresis ..
Hi
Hi
Mam.u r best teacher..u teach very well.and u r very pretty
you are a great great teacher i learned from this video more than I did in university
zindagi me pehli bar koi cheej itne ache se clear hui hai
I got my job...they asked me about HPLC ...love from bangladesh đđđ
wow congratulations !!
which company? where u come from?
Mem , please make Electrometric method Videos....
Thank you.đ
Thank you so much for making this vid ma'am... Best ever explanation on CZcams đ
Thank you so much ma'am ...maine aaj hi aapka channel dekha h aur aapne bhaut acche se smjhaya hua h .... Kl mera exam h ...m really very happy i was tired of reading the books n all stuff but this is very interesting ...đș
I'm from Pakistan your lectures are very helpful thanks Madame.
Thank You Naseer đ, glad to hear that my videos are being watched in neighbouring countries đđ»đ
Ya
Me also
@@BaaYo yes mam your way of teaching is very good and easy....everyone can understand it....mam kindly also mention tge name of buffer solution and mqke video on chiral chromatography đ respect from pak
Thanks mam very informative and clear plz make a video on how to standerdization hplc machine for to check diffre t component in a blood sample thanking you
Nice
thank you so much.. tomorrow is my presentation , this video is very very helpful for me
Thank you so much Ma'am.... Allhamdulliah....I have learned a lot about HPLC after watching this vedio.... Mashallah.....it's just because of Allah almighty and then by your Wonderful lecture....
Excellent mam
Tq
Good v good
To access notes and practice questions, please join our Telegram channel t.me/baayo_official
Ma'am I really like the way you teach... Your way of teaching is just as beautiful as youâ€ïž
Remarkable way of explanation mam. Kindly upload lectures on Electrophoresis too. I couldn't finf any of your lecture on Electrophoresis. You explain each any everything very precisely. After taking your lectures not getting other lecturers .
Super mam. ELISA per Video banav
Thanks laxmikant.. Jaldi bnaugi..:)
Hi Guys, Please watch our new videos on
Mass Spectrometry czcams.com/video/w3LS22E10Zc/video.html
UV- vis spectroscopy links.. czcams.com/video/1mrUHrZ-0zk/video.html
Gas Chromatography czcams.com/video/0KqaIKHzHE8/video.html
and please share your valuable feedback. Thanks.
U r cool..
Keep it up
Pls make a video to calculate peak area in HPLC and how to quantify?
Noted. surely will make one. thanks
Thankuuuu sooo much mam... U can teach all chomatography methods .... U r very great teacher thankuuu so much
Hats off to u.... You r leaving your child crying for making videos for us
Wonderfully delivered ma'am,,
I am on the edge of completion of my Doctrol studies at NIT Surat...and my entire work is on natural products chemistry...
These lecture series may play a good role to improve my basics...
Please share your email or linkedIn id maam!
@@BaaYo thank you ma'am
Jo ki hydrophobic the
Your explanation is very easy and to the point, thanks for explaining
You are doing great job mam , you have our respect
Thanking you from the bottom of my heart đđđđ
I wish this had english subtitles :(
LMAO. I wasn't sure if she was jumping in and out of English or her accent was just that heavy. I thought she just had a heavy accent
Maa'm plzzzz make A video On UV spectroscopy đđđđđđ plz itsss request maa'm
Hi Lovepreet, Sorry for the delay. I was on break for two weeks, I will make video on this soon and upload it. Thanks for follow up.
Ha mam uv pe banaiye jaldi
Hi Prashant, UV Visible Sepctroscopy video is live now.
Watch it on below link:
czcams.com/video/1mrUHrZ-0zk/video.html
Hi Lovepreet,
Based on your request, UV Visible Spectroscopy video is upload now :). Watch it at below link:
czcams.com/video/1mrUHrZ-0zk/video.html
@@BaaYo thanks a lot maa'm đđđđđđđđđđđđđ
Thank you so much ma'am for explaining HPLC so well.. you are great â€
Amazing explanation mam...simply amazing of u ...u make the topic easy n short to understand. Less time is consumed for understanding a particular topic. thank you mam
So simply and more effective explanation
Thank you so much Ma'am .. finally many of my doubts clear now.. đ
Hi, This video is really helpful for me. Keep making these types of resourceful video. Thank you so much.
Excellent video and clear and concise presentation. All the best for future work. I have a question, if it is possible for you.. could you please make a small video on how to interpret the chromatogram using actually HPLC equipment?
Excellent explanation, thank you for sharing the knowledge
Very effective lecture maâam thanks a lot for providing such a great infoâ„ïž
The concepts about instrumentations explained by ma'am help me a lot in my examination
Thank you ! you explained in a very easy understanding way.
You are the best discriber of instruments in my knowledge
Crisp explanation of hplc.
Extremely helpful.Thank you Mam
Superb explanation mam.thank u so much . U r such great teacher .hats off u
thank you mam im student from final year b pharm . you explianed hplc in very simpler way just like a basic hint about it . really helpful as due to lockdown this online coaching cuase difficulty in understanding concept but you made it simpler . again nicely done