Gas Chromatography Principle and Instrumentation
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- čas přidán 23. 08. 2018
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Gas Chromatography in Hindi, Gas chromatography principle, Gas chromatography instrumentation, Mobile phase in Gas Chromatography, Stationary Phase in Gas Chromatography, Gas Liquid Chromatography, Gas solid chromatography, GCMS, Gas chromatography detectors, gas chromatography mass spectroscopy
This chromatography lecture explains about the instrumentation, principle of gas chromatography. Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture .
This lecture will explain the principle of gas chromatography and the instrumentation and how to use gas chromatography.
In gas chromatography, the mobile phase (or "moving phase") is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen. Helium remains the most commonly used carrier gas in about 90% of instruments although hydrogen is preferred for improved separations.The stationary phase is a microscopic layer of liquid or an inert solid support, inside a piece of glass or metal tubing called a column. The instrument used to perform gas chromatography is called a gas chromatograph.
Gas chromatography is in principle similar to column chromatography but has several notable differences. First, the process of separating the compounds in a mixture is carried out between a liquid stationary phase and a gas mobile phase, whereas in column chromatography the stationary phase is a solid and the mobile phase is a liquid. (Hence the full name of the procedure is "Gas-liquid chromatography", referring to the mobile and stationary phases, respectively.) Second, the column through which the gas phase passes is located in an oven where the temperature of the gas can be controlled, whereas column chromatography has no such temperature control. Finally, the concentration of a compound in the gas phase is solely a function of the vapor pressure of the gas.
Gas chromatography is also similar to fractional distillation, since both processes separate the components of a mixture primarily based on boiling point (or vapor pressure) differences.
Gas chromatography is also sometimes known as vapor-phase chromatography (VPC), or gas-liquid partition chromatography (GLPC). These alternative names, as well as their respective abbreviations, are frequently used in scientific literature. Strictly speaking, GLPC is the most correct terminology, and is thus preferred by many authors.
In most modern GC-MS systems (GCMS, when mass spectroscopy is used as a detector), computer software is used to draw and integrate peaks, and match MS spectra to library spectra.
Article source: wikipedia
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thnx mam
हमारी राष्ट्रभाषा में अतीउत्तम तरीके से गैस क्रोमैटोग्राफी समझाने के लिए धन्यवाद।🥰🥰
Dhanyavad Akash :)
Akash bhai hindi rashtrabhasha nahi hai. :)
@@imrocknreeling 😂
@@imrocknreeling akash ko Hindi bhi likhni nahi aati proper
Tq maim ji
I have my first interview tomorrow with focus on Gas chromatography and Liguid Chromatography....I dont have previous knowledge about this but your video is very helping,,,im seeing your videos again and again so i will have a strong understanding and i can do well in my interveiw...Thanks for explaining in such a nice way...
How was interview?
How was your interview
Interview about chromatography😂 icant believe🥴🥴
Kuch bii ?😂😂
@@abadsoofi yes, for Hplc GC analyst, they conduct interviews lol
Arti your way of explaining the subject is amazing. Its so simple and understandable. I was writing my Pre-Phd examinations of at the age of 53, and was struggling to read through text books and then I found your videos. I am from South India, even though I know Hindi but not so comfortable. But, your language and way you were explaining was amazing. I wrote my exams yesterday and I am very much satisfied with the way I have written and definitely credit goes to you. Thanks a lot and will keep watching your videos whenever required.
The way of explaining is really really good & very well explained. i wish, every college & school has a teacher as like you M'am. it's really important for those who are the genuine learners.
i wish the same , thanks a lot BaaYoo .
:)
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Honestly speaking the way of your explanation is best.Teachers like you are very rare.I watched your other videos as well,lot of love and respect for u dearest mam.God bless u.
I needed to strengthen my basics over GC MS for a project
I'm so glad for this video
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Thank you so much
My heartfelt gratitude 🙏🏻❤
You must be someone working in a reputed organization..Your explanation clearly reflects your talent. ...
This video helped me in clearing my 1st interview 😍❤️❤️❤️❤️Thank you so much🥰
In which company bro
Gous Inamdar Its an chemical company bro located in mumbai
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Gous Inamdar its an small company, For freshers its 10 k
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Thanks again from KOREA
Than u so much mam.
Stay blessed...
Initially you have mentioned that GC or GLC is a partitioning based chromatography. This concept never appeared later and I think that is the most important factor in separation. A low boiling liquid may elute later depending on partition coefficient. That is the reason different columns are employed for different type of mixtures. Partition coefficient is also affected by polarity of the solid phase. Anyway with GC most common detector is FID (Flame ionization Detector).
Its is very useful videos who has not understand basic concepts and mechanisam of what's is gng in GC...I listened 3 times continuously so that I assured I have not missed any small important point..Thank you for sharing ur knowledge to me and I wish to continue your journey for more topics..stay happy..
Thank you so much mam😊
You just made it looks so easy... 🙏
ur looks made more easy to understand 😍
Hey i am from uk and learnt more in detail from you. Thank you, mam god, bless u.
Huge respect to you mam. The way you explains the subject is unique and easy to understand.
Thanks mam ☺️
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😘😘 madam college se jyada perfect knowledge aapse milta hai.... Madam fid, ecd, fpd, madam ye detectors b explain kijiye
Thanks Mam, you solved almost 95% problems about chromatography , i saw all type of chromatographic lectures
i am from Pakistan ,,
Nicely explained. Thank you very much. Read in college long before. Now i need to understand for my plant.
Wow... Mam.. Huge respect to u.
For the first time I understood a class in Hindi language fully.The concepts explained in a very clear way .Thank you very much.
Thank you 😊🙏
@@BaaYo Allah Pak aap ko salamat rakhy
I couldn't understand Gas Chromatography but afer seeing this concept with the help of given vedio I have cleared my all doubts very easily I help me much.
very nice. I tried many videos to clarify concepts but this video made everything clear. Brilliant
Thanks a bunch, ma'am! I've been watching your videos for analytical pharmacy and they're of a HUGE help! Stay safe!
I think this is what you came for..awesome explanation and to the point😃😎😎
Thanks 😊😊
You can't believe how much you helped me to understand the topic
For my exm which is tomorrow
Thanks mam 🙏
Thank u so much I watched many video's before watching this to prepare my notes for sem but ur explanation help me a lot
nice lecture.I watch your every lecture and i have learned So many difficult topics.Thanx From PAKISTAN
Thanks Wahid 😊... Glad to hear that
Love that way to talking and giving concept clearly and easly
You are a best tutor and I hv see all the videos of you which is helpful for me thank uh Aarti ...😊😊☺️
Iam Very thankful to you
Mein subh se is topic ko book se samjhne ki koshish kr rhi thi finally sham ko CZcams ye vedio dekh li ab sab smjh aagya great explanation 👏👏👏👏❤️❤️
मैं तो पहली बार समझ पाया हूँ । बहुत बहुत धन्यवाद मैडम जी। अब लगता है कुछ आया हमे
Thank You so much :) and Wish you a Happy New Year :)
@@BaaYo happy new year 2019 to you and your sweet family. With best wishes
this is real learning
Wow.. Mind blowing ma'am
Your way of explaining the things you too are just awesome ❤
Here is my request. Please, Can you talk more about GC Detectors such as Flame ionization detectors, TCD and ECD? Thank you for this video. I like your way to explain the principle of GC.
Yo, I don’t even know what language you’re speaking and it’s still making sense to me. You’re amazing
It’s Hindi (Indian language)
Bro It's hindi language...u can search about hindi on Google 👍
hindi language
I would suggest you come in contact with Indian and learn the Incredible Hindy language.
@@rktiwari5132 first you learn how to spell hindi
Best explanation ,sab kuch dimag me chap gaya hai , you are the best mam thank you so much
Thanks for ur Efforts, which brought me to understand the concept of GC. Excellent explanation....!👏
This is the method, how to teaches well mam appreciated
Awsm teaching maam,, really its helping me as I am a B.pharm final year student
Thank you 🙏🏻:)
Thank u so much mam
You are explain in very simple way☺ i have shown ur video when tomorrow is my exam.
Thank you for making clear my basic concept of chromatography
thank you from Bangladesh 🇧🇩🇧🇩❤❤
Dear Mam,
Hope you are alright.
Really appreciated from Pakistan. I'm in research business sometime I say your videos. You are doing an outstanding job.
Regards & Thanks
Raza khan
Mam pyaar bhi ho gya apse.. gas chromatography smjhte smjhte ❤️
Nice dii
bhot bda vala hai tu saaa..........le.....
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@@summeralbum2741 well said.
😂😂😂😂
Going to work on it tomorrow.
Thanks for making things this easy.
Tysm for this mam....jo mahino se samjh nhi aaya wo ek hi baar me smjha dia aapne. Tysm mam .
With your lecture every topic become easy to understand
Thank you for such an informative video
Please give references also.....
Yes I'm also gt very easy about gc tq u mam
Nice
very easy method..tnx Mam
Mam.please make a video on HPLC instruments ..please
Right
Very nice and interesting video. Thank you so much madam for your kind co operation.
I have understood about the gas chromatography
The way of explaining is very good
Thank you so much 💗💗
Please make a video on X-ray Crystallography!!!
wish u were my instrumental teacher. 💓
😂
Very well explained my all confusion are out of my mid thankyou
VERY NICE AND BEAUTIFUL , THANKS FOR SHARING , WAITING FOR MORE , THANKS A LOT
Tomorrow is my Instrumental technique of analysis's exam .
This gonna help me .
Thank you so much mam .
Please explain thermogravimetric method of analysis
Thanks so much Mam g your Lecture are very helpful....Hum Types of Chromatography study kr rhy hen r ap k Lectures sy bahut zyada help mil rhi ha...Again Thanks Ma'am g
Your videos on Instrumentation and biotechniques are helping me a lot.
Thank you..
Mam gc m to many types of detector use hote h uus PR v please 1video bnaye
Thank you so much !
Can you please explain on which factor the selection of GC and HPLC depends?
Means how to select GC or HPLC for particular component mixture
Literally thank you ma'am...! just smoothly understood everything now ...!
Isse best explanation ho hi nahi skta tha ma'am 😊
Thanks lottt
Nice explanation mam
Thank You Pawan. Please share this video with your friends also.
Mam thanks.ur style of explaining is so nice
thanks best understand
Thank You 😊
thank you mam.....
apke sikhaneki speech and technic bhi sahi he ....jo jaldi se samaj sakte he.....
Mam, I m in love with your explanations and presentations but you are always in fast pace with a anxious way...
It helped me a lot...........😇 after this no doubts🤗
How stationary phase helps to separate the molecules (?) as the molecules are separating on basis of their boiling point .
Please reply
Thank you🙏💕
here polarity will also work If the polarity of the stationary phase and compound are similar, the retention time increases because the compound interacts stronger with the stationary phase. As a result, polar compounds have long retention times on polar stationary phases and shorter retention times on non-polar columns using the same temperature.
You explained it in so well and expressive way...easy to understand.. Thanks a lot ma'am 😊😊
Thank you ma'am, I seen your good video 2nd time. I gain too much knowledge.
The perfect.
kash ap jesa teacher hme prhata to cpga 4 out of 4 aati.
but unfortunately jo prof. hme prha re hain unhn ne HPLC ko hath tk nai lgaya, r hme keh diya k khud tyr kr lna.
Thank You Bilal 😊, wish you a very Happy New Year
U r really too good in teaching , very conceptual and easy way explanation🙏
All concepts are clear after watching this video
Mam column chromatography ke upar video banao plz😊😊
You are sooo amazingg. GD Christian ne tou dimagh kharab krdia. 😢
very helpfull video, clears the concept of GC-MS Thanks
When I was watching your this video I prey from Allah to have you like wife after graduation 😎😎😎BTW you teach this topic more clearly and conceptual than our professor of university 😂😂😊😊live long and be happy always
Mam plz explained Affinity chromatography
Plz mam upload a video on affinity chromatograph 😄
Yes
its live now at the given link : czcams.com/video/6fMW0hLqTi8/video.html
Mam ur art of teaching is next level ❤️❤️....can I get Ur insta user id so that I can reach you out ?
Bahut tej ho rahe ho 🤣
@@atulnikam187 hona phdta hai 😂😂
Type of explanation is fabulous mam ...God bless you
Very productive video. Thank you 👍👍
its amazing and simple way to explain
Thank u mam. It was really very helpful.. now I can understand Dissolve Gas analysis of transformer oil. Thank u now I understand the principle
Thank you, mam
Your teaching technique was really amazing one
Thank u so much mam 🙏🙏🙏🙏 aap jese teachero ki vjh se ham itnaa sikh paa rhe he aage bad paa rhe 🙏🙏🙏🙏 bahut bahut dhanyvad
Thank you so much mam. You cleared my all doubt in chromatography
You made the topic so clear... Thankyou! 👍👍
Thank you so much mam for giving such type of amazing contest ❤
How can someone look so graceful 😍
What a great explanation method.
Keep it up mam. Your conversation method is as beautiful as you.
Thank you mam, humaare sir ki 2 ghante ki class me kuch samjh nahi aaya tha...Par aapne achhe se Samjhaya Dhanyawad ☺️
Mam your lecture is so best on gas chromatography, l got concept from your lecture. Thank you mam
Sidhi baat, no bakwas...awesome
Explain very well ❤ thankyou 🧿