Thank you for posting this. It seems like an open flame that torches the containers' brims was unnecessary and it's okay to hover over the explants. I was taught with these cautions in school but I want to improve my efficiency by simplifying the process. This video is informative.
Thank you so much for sharing this info! Slowly making the transition from growing culinary mushroom culture (reishi, lions mane, oysters) to plant tissue culture. Rare to find these helpful videos for cacti plant tissue culture techniques! Great job and very informative! Hope to find some MS cacti media (and woody plant culture) recipe videos someday :-)
Dude you are awesome, this is a really great and helpful set of informative and detailed videos for such a difficult to expand class of cacti. I really appreciate you doing this. Since I own most of this equipment already, I couldn't be more excited to start trying this.
I would suggest if you use a sterile 240ml wide mouth bottle with a cap so you can rinse your explant with dist H2O 3x by shaking then decant the water thoroughly. You have too many paper on top of the bench. Cut it into smaller pieces bond paper size (sterile) to carry out your disection of the explant if you do not want to use a petri dish. Bec us we are used to petri dish. There is an effect on the growth of the explant with the size of the tissue vs container to volume ratio of culture medium. You need to maximize the growth in short period of time.
I’ve been waiting for you to post more videos! lol i love watching your content. I have access to a 7 year old lop and i was wondering if you think it’s still possible for me to clone it?
Do you use PGRs in your media? I’m about to start a large tissue culture operation of palo santo but definitely want to branch out into xerophiles and carnivores, and for the palo santo I will be trying somatic embryogenesis and from there using a bunch of pgrs to pump shoots, etc
@cactiFanatic do you have a list of hormones and where to get them. Such as a package deal where I can buy them all? I’m not tech savy so I don’t really know much out online stuff. Took me forever to find this video again. And another couple days to figure out how to comment on CZcams. Do you have an email?
great job, when using the MS medium do you happen to add any other growing hormones for specific growth? would you suggest starting out at half strength ms powder? good looking out.
So if I were to TC dragon fruit varieties, would my explants need the actual stem from the middle, or just the spines where new growth occurs? Are bigger or smaller chunks better? Do you know if contaminants can get inside the container if it's sealed? Thanks so much for this video. It has helped me tremendously.
The smaller the piece contains the meristem the better but on the other hand having more tissue they survives with the meristem encourages faster growth. More tissue increase contamination chances. No they contamination does not get through. All containers are wrapped with micropore tape of moving to regular non clean room environment
Question, lophophoras are painfully slow in their own roots, This accelerates that or just helps with propagation? Also you should try tissue culture with seeds
What about the media. I want to clone Trichocereus cacti. I get the basic idea. I am unsure what media. Is there a protocal for cloning cactus that is not behind a paywall?
Yeah!! It’s online free. Just use a MS based media with vitamins, 30g sugar, activated charcoal and agar to set. That’s good enough for basic tissue culture then maybe BAP to induce
One of the best videos on plant tissue culture procedures where everything is clearly explained that I have seen on CZcams 👍
Thank you for posting this. It seems like an open flame that torches the containers' brims was unnecessary and it's okay to hover over the explants. I was taught with these cautions in school but I want to improve my efficiency by simplifying the process. This video is informative.
Awesome videos! Finally someone demonstrating tissue culture on cacti. I can't wait for your next video.
This was an amazing video! You did an excellent job of covering all the details. I look forward to more of your videos on this subject.
Thank you so much for sharing this info! Slowly making the transition from growing culinary mushroom culture (reishi, lions mane, oysters) to plant tissue culture. Rare to find these helpful videos for cacti plant tissue culture techniques! Great job and very informative! Hope to find some MS cacti media (and woody plant culture) recipe videos someday :-)
Regular base media works for almost everything. Some special medias work better or just marketing
Dude you are awesome, this is a really great and helpful set of informative and detailed videos for such a difficult to expand class of cacti. I really appreciate you doing this. Since I own most of this equipment already, I couldn't be more excited to start trying this.
I would suggest if you use a sterile 240ml wide mouth bottle with a cap so you can rinse your explant with dist H2O 3x by shaking then decant the water thoroughly. You have too many paper on top of the bench. Cut it into smaller pieces bond paper size (sterile) to carry out your disection of the explant if you do not want to use a petri dish. Bec us we are used to petri dish. There is an effect on the growth of the explant with the size of the tissue vs container to volume ratio of culture medium. You need to maximize the growth in short period of time.
To each there own. I have many many many vessels. That’s a sterile drape.
Thanks for sharing your knowledge,
dude i love this!
Loved the video, did you trim the explant material that was exposed to bleach after rinsing, before cutting down into smaller pieces?
One day ago i thought the same about sissors :D Great videos thx
How do you cut up a leaf from a Thanksgiving cactus?
I’ve been waiting for you to post more videos! lol i love watching your content. I have access to a 7 year old lop and i was wondering if you think it’s still possible for me to clone it?
Yeah any age can be done but the older the harder because it breaks my heart haha
Where do we buy those containers?
Do you use PGRs in your media? I’m about to start a large tissue culture operation of palo santo but definitely want to branch out into xerophiles and carnivores, and for the palo santo I will be trying somatic embryogenesis and from there using a bunch of pgrs to pump shoots, etc
Sometimes on my patreon I go more into the hormones and when I’m using them
@cactiFanatic do you have a list of hormones and where to get them. Such as a package deal where I can buy them all? I’m not tech savy so I don’t really know much out online stuff. Took me forever to find this video again. And another couple days to figure out how to comment on CZcams. Do you have an email?
Yeah email me at cactifanatici@gmail.com
My patreon has that list and info
I've watched literally every video you've made. I still have no idea how to TC cacti. What's your multiplication media? Nobody knows!
*brings up sci-hub*
great job, when using the MS medium do you happen to add any other growing hormones for specific growth? would you suggest starting out at half strength ms powder? good looking out.
Honestly for cactus they don’t require any hormones once they get going but I do add to speed up things
appreciate the video. for dragonfruit, can you only use the spines and not the rest of the plant?
You could but it’s very long and difficult path. Best to use nodal / meristematic tissue like
Spines / aeroles in cactus
Great video!
Most important question: what medium do you use for this?
MS based
So if I were to TC dragon fruit varieties, would my explants need the actual stem from the middle, or just the spines where new growth occurs? Are bigger or smaller chunks better? Do you know if contaminants can get inside the container if it's sealed? Thanks so much for this video. It has helped me tremendously.
The smaller the piece contains the meristem the better but on the other hand having more tissue they survives with the meristem encourages faster growth. More tissue increase contamination chances.
No they contamination does not get through. All containers are wrapped with micropore tape of moving to regular non clean room environment
so u can do this with any other cacti? can i do this with like copiapoa cinerea?
Just posted on my Patreon for copiapoa ;)
Question, lophophoras are painfully slow in their own roots, This accelerates that or just helps with propagation? Also you should try tissue culture with seeds
Yes I have over 100 started from seed. They are very fast in TC compared to regular in my opinion but still not as fast as a pereskiopsis graft
It's would be interesting to know if alkaloid content differs or if it's just water weight.
Do you use some equipment like exhaust ventilation to be sterile?
I have a FFU but the work can be done in a still air box set up also
What do you call your containers you put ur TC in or where did you purchase them?
Sterile deli containers
What the media you using?
MS based
What's your media recipe?
Not going to wine and dine me first!!! Lol
It’s MS base media with vitamins : classic recipe
@@cactifanatici Oh I happily would lmao! Thank you!
MS with 1mg BAP ..
I was told you don't need it if you are using the Gamborg B5. The media is for callous plants like most cacti. 👍😁
What about the media. I want to clone Trichocereus cacti. I get the basic idea. I am unsure what media. Is there a protocal for cloning cactus that is not behind a paywall?
Yeah!! It’s online free. Just use a MS based media with vitamins, 30g sugar, activated charcoal and agar to set. That’s good enough for basic tissue culture then maybe BAP to induce