Tissue Culturing Aloe Vera!
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- čas přidán 3. 02. 2021
- Aloe Vera is a very popular houseplant and one of the easiest to introduce to tissue culture. For that reason, in this video we are going to show you how to do the tissue culture of Aloeveras.
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Thank you very much for this informative video.
Stay tuned to our channel for more videos like this.
Do try Nepenthes. Anyway your headset could make a trendsetter in a lab.
Truncata, Veitchii, and Spatulata x Spectabilis coming soon.
Good thanks
no problem, happy culturing!
Thanks for this video. Could you make orchid nod sterilization protokol? (My be dendrobium nod or phal nod, or each others)
Yes, I really want to work with Orchids!
Excellent sir! Thank you for sharing. You could do the sterilization step on papaya? It would be ideal for ending my degree!
From plant or seed?
Thanks for the video. Please make a video for media preparation for aloe vera and the composition of the media. Also sterilization, composition and media preparation of pothos, aglaonema and hoya compacta.
I'm currently using normal MS media, I'll move the plants to a more specialized media soon. I do have the other plants on my list already!
@@PlantCellTechnology Thanks for your reply. Waiting eagerly for your other videos coming up.
I have another question regarding the sterilization process. My explants (pothos, hoya compacta) are turning black (looks dead) after following the sterilization process(1hr dish soap+1 hr 1% bleach + 5 min 3% H2o2). Any suggestion?
Hola, me encantaria si lo pudieras hacer con philodendron y anthuriums. Muchas gracias!
Anthurium Flamingo este Martes, y Philodendron posiblemente en unas 2-3 semanas.
Make a video on how to subculture the plant and how we can make shooty and rooty media for orgenogenesis
Okay, will do!
Thanks for the informative video. I was wondering if it's possible to do vanilla planifolia tissue culture? Thanks!
Yes we can, let me get a plant first.
Thanks!!!
Thanks for making this video! Would you be able to do one for cactus?
Of course, but I'm not looking for it hahah.
I would also really like to see a video for cactus
thank you but you use big explant it seems shooted. can we use smaller explant that you used. please show us if other alternative method of preparation of explant.
Can you try with cactus plant such as trichocereus pachanoi, harisia jusberti and astrophytum.
I'm not too exited about working with cactus, but I'll do it sometime.
@@PlantCellTechnology Thank you, I really appreciate. I have tried cactus tissue culture but it is always contaminated.
thanks for the video! Please, begonia leaf explant esterilization!
Great suggestion!
Any video on phalaenopsis flower stalk?
Currently, we do not have any videos on the same. However, if we get our hands on the plants we will try to create a video.
Thanks for the suggestion.
Thank you for interesting vid! Could you do video for orchids?
We will try in the future.
Can you share the tissue culture techniques of aquatic plants
Is there any specific plant you are looking for? Try and explore our channel to find some videos on aquatic plants that we have already done before. They might be helpful for you!
make video with microprapogation of ginger using sprout as explant
I do have some ginger, I'll give it a try!
I have a question can I use a flowering cannabis plant 5 weeks into flowering as an explant ?
Yes, it's possible, but it's not ideal. I would let it flower, then cut the plant back, and increase the photoperiod to +18 hours per day. That way it will grow back in vegetative form.
Could you show how to do this with banana tissue?
Unfortunately, we don't work on bananas in our lab. But, you can find many other available resources on the internet.
Do you know how to in vitro germinate cannabis seeds? (love ur videos)
I do, but it uses a very nasty chemical. It’s quite dangerous, so I won’t share a protocol until I found something safer.
@@PlantCellTechnology ok got it. Thank you for u answer
How do you sterilize your magnet once used
you can use ethanol.
What is the concentration of vinegar used? I need to find a product similar to this one, as this brand is not found in my country.
1% volume of cleaning vinegar is used in the video.
Cleaning vinegar is 6% acetic acid and food grade vinegar is 5%. that does not seen like a lot, but that is 20x stronger!
@@PlantCellTechnology
Can you please clarify the purpose/action of the vinegar after the peroxide?
@@greencleanenvironmentalsol7053 It helps clean up the plant tissue.
What brand is your flow hood?
Need to confirm with Francisco. I will let you know.
It is Thermo Forma model 1845.
Why you don't remove damage area due to chlorox from the explant?
Because I use a very mild solution 1% bleach.
What komposition the media... Have you add hormone sir ??
MS media with 25 g of sucrose, 1 ml/l BA, 0.1 ml/l NAA, 1 ml/l PPM, and 3 g of Gallan gum.
@@PlantCellTechnology Hi, how many of BAP/ANA in mg/L? Thanks!
@@nazarenoarce4197 MS media with 25 g of sucrose, 1 ml/l BA, 0.1 ml/l NAA, 1 ml/l PPM, and 3 g of Gallan gum.
Kindly take stevia plant as tessue culture
Sure! We will try to bring it to any of our future videos.
Thanks,Waiting...
I think callus methods is best
Do an ornamental plant like chrysanthemum.
Can you elaborate on your question?
@@PlantCellTechnology I'd like to see you tissue culture Chrysanthemum plants
@@realalsingh-ramharrack8762 Sure! The informative videos on different plants will keep coming!
Best way to do cannabis sterilization? Thanks
I saw you are using 1 % bleach.
Is 1 % bleach better than 10% bleach for delicate plant like this?
Yes, I use a mild solution for longer time. Won't say it is better, but if it get contaminated, you have a second chance to resterilize the explant.