Inoculating Liquid Bacterial Culture

Inoculation is the process by which you introduce bacteria into liquid media. In this video we will demonstrate best practices to perform inoculation, ensuring sufficient numbers of bacteria for plasmid DNA isolation.
For the full written Inoculations protocol, visit:
www.addgene.org/protocols/ino...
For our DNA Isolation Protocol, visit:
www.addgene.org/protocols/pur...
For our Creating Bacterial Glycerol Stocks protocol, visit:
www.addgene.org/protocols/cre...
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0:00 Intro
0:48 Materials
1:29 Setup
2:34 Inoculation
4:34 Incubation
5:34 Post-Incubation
5:43 Troubleshooting

[@addgene]

Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to [e-mail] as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!

[@lovelysoni1829]

Nice video for kind information thanks again very much to you

[@koalasaw]

very helpful thank you

[@laxmikiruthikamanoharan1768]

Thank u

[@TALKyLIKEENGLISH]

so we should after transformation: liquid medium --> plate --> liquid medium. What if we skip the first step?

[@akshitasoni445]

Should i use nutrient broth instead of LB for Bacterial DNA Isolation @Addgene

[@nitishkumarprajapati7115]

How to culture Sporosarcina pasteurii Bacteria??
Pls make video....

[@jejeoluwatimilehin8918]

in a situation where we use gentamycin, kanamycin and rif why is this?

[@janebond3263]

how to storage with glicerol?

[@funny11744]

Please provide here the link with quantities of antibiotics for culture . Thanks

[@addgene]

Thanks for watching! You can find a chart of antibiotic concentrations here: www.addgene.org/protocols/inoculate-bacterial-culture/

[@jasonbattermann9982]

Why do so many education institutions call LB lysogeny broth, but companies use the term luria broth?

[@impulsessj2033]

luria is the person who created the formula.

[@devanisasankan818]

👍👍

[@nikilee8862]

why do you need antibiotic?

[@anjalisharma314]

Sir I have to incubate an organic liquid of 50mg/L with a bacteria. Please tell me the procedure for it.

[@addgene]

Thank you for your question! You might find the protocol associated with this video helpful www.addgene.org/protocols/inoculate-bacterial-culture/

[@anjalisharma314]

@@addgene thanks for response sir but I want to know the degradation ability of a particular compound by a particular bacteria. Shall I apply the same procedure, that has been given in the link.?

[@addgene]

@@anjalisharma314 The protocol shared above is intended for growing up bacteria in liquid cultures and may be a good starting reference. However, Addgene does not perform functional experiments like the one you've described so we can't provide specific recommendations on the conditions you may need to use. Sorry we can't be of more direct assistance!

[@anjalisharma314]

@@addgene it's ok sir?

[@oulythorng3922]

Why don’t we take back the tooth stick out of the medium ?

[@addgene]

Thanks for your question! Leaving the toothpick in the culture tube avoids having to stick something else in the tube to remove it (e.g. tweezers), which could lead to contamination. Removing the toothpick once you've finished growing the culture is less risky because any contaminant won't have time to grow.

[@Mirracle74]

Elon Musk wants to inoculate the surface of Mars with H. sapien. I think that it’s a lousy idea because Mars isn’t the right growth medium for humans.

[@impulsessj2033]

my mans streaked it so wrong. you cant scratch the agar like that

[@Gilbertblythe292]

2023🦠🦠🦠🦠🦠🦠🦠🦠🦠

[@Gilbertblythe292]

2023🦠🦠🦠🦠🦠🦠🦠🦠🦠