Bacterial Transformations

Transformation is the process by which foreign DNA is introduced into a bacterial cell. In this video, we walk you through a standard plasmid transformation protocol and offer some tips and tricks to reduce transformation time and increase efficiency.
For the full written Bacterial Transformations protocol, visit:
www.addgene.org/protocols/bac...
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0:00 Intro
0:47 Starting the Transformation Process
1:41 Performing the Transformation
3:58 Incubation
4:31 Tips

[@addgene]

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[@Cartoons_90s499]

nice video i learn so much

[@infinity2394]

nice vid 👍

[@jeanelmercado1642]

I have one question Ma'am. When scraping and transferring the E. coli bacteria from the E. coli starter plate to the (+)BLU and the (-)BLU tubes, why is it important that you not scrape up any of the agar in the process?

[@addgene]

In general, it is best practice to scrape agar plates gently because the agar can easily be disturbed while collecting your bacterial cells. The inclusion of agar (or any components that may be present within the agar such as X-gal) in your cellular suspension may adversely affect downstream processes or experiments.

[@thefenerbahcesk4156]

Do you have a video on Asceptic Technique?

[@addgene]

Not yet, but that sounds like it would be a great topic for a video. Stay tuned!

[@omerhayri999]

SA

[@kaysoncanaan5542]

you all probably dont care at all but does anyone know a method to get back into an instagram account?
I stupidly lost my account password. I would appreciate any tricks you can give me

[@tatumblaine9089]

@Kayson Canaan Instablaster :)

[@thefenerbahcesk4156]

@@omerhayri999 AS gardaş

[@binusbn5681]

Is it nutrient agar or mha?

[@kaustavmitra4210]

Mam..... how does simultaneous exposure of the cell to ice and hot water bath.... increase the chances of transformation?

[@wayne02058]

The precise mechanism is not known. But the theory is they the cell walls open up and become "leaky" allowing transformation to happen

[@kaustavmitra4210]

Thank you mam