Making Luminol
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- čas přidán 4. 03. 2016
- Hey guys, this is the last part of the luminol synthesis. It has been a long time coming and I am sorry for the mega delay!
Luminol Recipe from: www.chm.bris.ac.uk/webprojects...
3-nitrphthalic acid synthesis video: • Making 3-nitrophthalic...
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Nile talks about lab safety: • Chemistry is dangerous. - Věda a technologie
Great! Now I can make luminol and test if I cleaned the blood enough.
Riddim Dubstep 😂
Muriatic acid is pretty good at cleaning blood from semi porous surfaces like concrete, but it will also attack the concrete. After that sodium hydroxide can be used to destroy trace proteins.
@@nunyabisnass1141 thanks! Exactly what I needed ;-D
Jimmy Tremblay yeah just so we're clear, you're a hunter, and you hang the carcass in your garage...right? Lol
@@nunyabisnass1141 call me a... contractor... of sorts...
I do hang the carcasses in my garage, thanks for asking :-D
excellent work! way better and cleaner than my approach.
+NurdRage Damn, that means a lot coming from you. I also think this is the first time that you've commented on a video of mine :)
+NurdRage you guys should do a collaborative video together! That'd be amazing to see!
+Nile Red It's funny thought that NurdRage was you and you were saying that your approach was better than... your approach. It's the NR initials. ;)
+Bane? NR means No Reaction
+NurdRage I totally read this in your voice!
If you ever make t-shirts, one idea is to have the words, "We'll just have to pretend that it's there."
i think an "anyway" shirt would be awesome too lol
"ice old ethanol" tshirt would be the best lol
"Wash it with distilled water"
"In hindsight, I should have used a larger container"
@@krissp8712 "the yield is extremely low, and I don't know why"
me: where have I heard luminol before.....???
me: (remembers my Ace Attorney phase)
me: ah. right.
Which one? DD?
I think luminol was introduced in in last case of first game, and reappeared in fourth game and ME:AAI spin-offs (and I think it should appear once more in sixth game)
mood ngl
I heard about it when I was watching CSI.
I was thinking the same thing
I don't know how anyone can dislike Nile's videos they're great, interesting, educating and entertaining
Someone who doesn't understand the chemistry and can't appreciate the effort he puts in.
@@Hezarou or they don’t like the editing style, lack of closed captioning etc. not everything is black and white and not everyone has to like everything you do
@@cadenbrown7407 what? No, I'm not talking about appreciating the work he puts in; the effort. You can dislike the style, the captions, hell you can dislike Nile himself. But you can't deny he does what he loves and he puts effort in, I always appreciate people who put effort in what they like. So don't think everyone you meet online is as shallow as you believe they are.
It could also be possible, that people accidently klick dislike. I also had it a few times, that I opened a video and I suddenly saw, that I disliked it before accidently without noticing🤔
I can't imagine, that anyone would dislike this!
@@Hezarou You totally missed the point and reiterated a prior opinion. Excellent comprehension.
I really hope you do a video of luminol reacting with blood. I am currently working towards a Forensic Science major at Wichita State University, and am highly interested in chemistry as well, so I'd love to see a video that applies to both :D
You still in Wichita
@@HooyahPeacock I sure am.
Me too
@@HooyahPeacock That's cool. I'm actually still at WSU for forensic science. I'm getting my bachelor's at the end of the year.
@@AmusedToast COME TO LOS ANGELES, WE HAVE PLENTY OF COLD CASE FILES TO SOLVE, INCLUDING Y FRIEND LUCIA, WHO SUPPOSEDLY SHOT HER SELF IN THE HEAD WITH HER SHERIFF BOY FRIEND, HE WAS ALWAYS A TOTAL JERK OF A GUY, AND I NEVER TRUSTED, OR RESPECTED HIM AS A PERSON. LUCIA HAD A TWO YEAR OLD, THAT SHE LOVED TO DEATH, I KNEW LUCIA WELL, LEA REMEDY JUST MOVED TO HOLLYWOOD SCIENTOLOGY, TO PURSUE HER ACTING CAREER, AND STARTED TO HANG OUT WITH US AT LUCIA’S HOUSE, AND GOT TO KNOW WHAT A JERK LUCIA’S SHERIFF BIY FRIEND WAS. I BELIEVE IT WENT DOWN AT HIS HOUSE IN STUDIO CITY. RIP LUCIA BENTTONCOURT.
Luminol produces a nice blue glow.
Your second channel is "creatively called" Nile Blue.
I think you should create a NB logo with luminol just like you did in this video with NR.
+Melody Williams That is actually a pretty good idea!
is this truly the comment that gave birth to the nile blue idea?
Clearly opaque... I see what you did there...
I am a wordsmith
krap101 i was about to comment this!
😭
Why is there a random Australian dude that cuts in at 15:47?
lol
Idk!
LMAO
I think it was just a voice taken from another video as he said 25 grams of what he was adding and then Nile repeated that.
Don't Even Bother
IT’S EXTRACTIONS&IRE
ecstasy.apostasy ITS ALSO EXPLOSIONS & FIRE
I like seeing your mistakes much more than successes. Thanks for keeping mistakes and sharing them with us.
Tasty mango smoothie
Edit: Lemonade looks good too
glowing gatorade
How much nilered has grown in the years is crazy, great job, keep it up
I can’t tell you how refreshing it is to see a video presented by someone that knows what they’re talking about, is clear and concise without the unnecessary theatrics you see when people are trying to turn themselves into a social media personality and/or sell you something. I definitely would be open to more detail/instruction on the reaction mechanisms.
I'm still waiting on the future video of you exploring luminol. Its been long enough Nigel.
Thank you so much! I had to find an original experiment for college o-chem and this is so helpful!
why couldnt my chemistry teacher make it this interesting i would've actually paid attention in class
Cause they don't know what they talking bout
Luminol is so cool and interesting! Thank you for making these videos!
this is my favorite video of yours nile! i love forensics :)
Fantastic results man!
It is 2:30 am December 2021. I've finished work, made the long drive home and I have chosen this video to watch. It's a 5 year old video at this point with 19 minutes of a pair of hands making a forensic liquid because I remembered how cool it looked on CSI.
The color it has when it first glows is my favorite color ever. I absolutely love that color.
I actually first learned about this chemical from playing ace attorney! So cool to see how it is made
same 😩
Followed this synth, one of the coolest things I've done.
Brovo! Some of your best work. :) keep it up!
I did this synthesis last week. From 200g of phtalic anhydride (pure p.a., Acros) i got 40 grams of 3-nitrophtalic acid. I took 10g of acid which yielded about 8g (6g after drying in oven) of luminol. All steps were identical, except I used pure sodium dithionite, cause it can be bought over the counter in Poland. Only last step was a bit different - I dumped the sodium dithionite to the solution, brought it to a short boil, and left it to cool. Great video.
Isn't Hydrazine a fuel used in spacecraft reaction motors?
Yes
That voice at 15:56 reminds me of "2 shots of vodka".
AND THERE YOU HAVE IT
Just want to let you know this is my favorite CZcams channel.
I love that you're using pieces of old papers you printed, been there.
Got interested in the synthesis playing Trauma Team and thought to myself "NileRed should synthesize and test luminol" then it turns out he already did! What a treat
+Nile Red At 7:50 I don't think the ammonium carbonate annotation is supposed to be there.
What other reducing agents do you think would work in the final reduction to make Luminol? Do you think sodium borohydride could reduce the nitro group?
Ah, the old heating the test-tube with a heat gun trick.
Had to do a diphenylacetylene and then hexaphenylbenzene synthesis recently which involved exactly that, except with little to no solvent.
I want you as my chemistry teacher!
If you start at n2h to nh2 before the vacuum pump would you be able to fallow the rest of the step as is or would it turn out a bit thicker or unusable
I've just discovered your channel and I'm completely blown away. This is proper hands on chemistry, a clear demonstration of various chemical reactions resulting in a variety of products using simple language that is accessible to anyone. In chemistry classes these days it feels like so much time is spent trying to get students to think in abstract terms about theory that they rarely if ever get to experience genuine practice. In my opinion this approach to learning is backwards; you shouldn't try to teach someone to do something before you do it, you should walk them through and SHOW them the process in action, and then seek to explain it in detail!
Mrjmaxted0291 yeah i completely agree
When luminol glows in the dark it’s just so fascinating
I follwed this procedure and was able to get 1.33 grams of luminol in my chem class, thanks nile red. But the oxidation didn't work out for me cause of lab misinterpreting ferricyanide with ferrocyanide so had to use bleach for that. I loved this experiment.
love it once again
Back in the early 1970's when I was in junior high school there was an (I'm guessing 1950's vintage?) chemistry experiments book in the school library that had a whole chapter on Chemiluminescence. It gave multiple formulas including this same one, and several others such as a deep red light made using Pyrogallol. As sources for the chemicals it listed Kodak, and also a Varniton Company located in Burbank California as I recall. Some other experiments shown were making pyrophoric lead, and dissolving cellulose then extruding Rayon. I'm just giving a couple examples from memory to see if maybe this rings a bell with anyone that may have the book or know the title and publisher info? I've always wanted to find a copy of this book, or to at least get a photocopy of it. Does anyone know of it? Thanks in advance.
If you wanted to do this reaction with pure hydrazine, what would change? could you just directly add the 3nitroacid thingy with hydrazine and have the rest of the reaction be the same?
That heat gun seems super useful.
hey Nile, I've been wondering- since glowsticks are a chemical reaction can you reverse it or I should say extract the luminal for reuse(if so how difficult would that be). thanks.
glowsticks dont use luminol or else why do they glow for more than an hour. we saw that it only lasts for about a minute so that's a stupid question
i dont have the sulphur agent. Could I first reduce the nitro group with HCl over zinc metal and then hydrazinate? Is there any guide to such approach? Thx.
Vacuum filtration looks so satisfying.
It's been 4 years. Did you forget about the luminol and blood experiment? I can't find it on Nile Blue either.
Is there any other way of reducing NO2 to NH2 by something else except of sodium dithionite? In my contry is not widely available :/
15:00 heh I made a video with those exact ingredients yesterday. I didn't know you made one :P
is there anything like thermoluminescent chemical that if you worm up compared to current temperature emits visible light but if you leave at this temperature slowly dims. if you cool down becomes black and cycle begins again...?
Wait a minute. If the max amount of the other chemicals is 66,5% how can the sodium dithionite be present in only 15% concentration?
How do you clean your buchner funnels? Are you using a paper filter on top of the frit? It's hard to tell from the video.
Excellent videos.
Incidentally, would it be easier to purify the sodium dithionite from your rust cleaner before you put it into the reaction, rather than having to deal with the foaming and having to repurify the luminol to deal with the additives? Or is it fine this way? You’ve done plenty of purifying and extracting from household stuff before, including a video on how to do a more general procedure, so is there some difficulty in isolating dithionite, or is it simply more practical to purify it afterwards?
Question, where do you get your vacuum filter? i found a hand pumped one that costs like $60 and i really thought it was a rip off so i didn't buy it
These videos get me through my anxiety attacks
Question:
Would you be able to change its color with it still remaining stable
Awesome video as always nile
it was pleasure to hear your real voice !!!
+Rajas Godbole but I always use my real voice?
+Nile Red He was referring to the voice that said "25 grams of sodium bicarbonate". Sounded Australian
Ludo Kressh haha it was my friend (who decided to put on an accent)
lol
After it stops glowing, is it like permanent or it'll start glowing again as we repour it to another beaker?
11:01 looks like slightly off orange juce
I'd drink it
Pete Lorenzo i wouldn't
sunnyD
The deadly orange juice...
Mmmmm refreshing
I am very curious as to what is the material that is used to filter on ur vacuum filter it’s a white substance that I am just not at all sure what it is I no u use coffee filters and other ones but I’m just not sure how the white stuff on the bottom that looks like powder doesn’t get mixed with whatever ur working with or what it is at all
Sodium dithionite is only metastable and rapidly decomposes in acid. My guess is the household version has the bicarb as a stabilizer. That's also why you get it as a possible range. Even the chem suppliers sell it as such and you usually use an excess. Again, nice hands NileRed. Mechanism is off, consider the first step going through the anhydride of the diacid (made by dehydration under the high heat). Second addition would also go through an anhydride or ester amide intermediate (from your glycerol or a dimer of the acids). Excellent demonstration.
That orange colour looks delicious
All I watch is forensic files and this is on every episode!!!!
Would there be any differences to using 5-nitrophthalhydrazide as opposed to the 3-nitrophthalhydrazide synthesized in thd video?
I always call heat guns industrial hairdryers. It make all the wood shop jockeys angry.
I understand why you filtered after the Iron out. But why did you filter before then? Wasn't all the materials before then, pure chemical ingredients?
The other day I saw a bottle of chloraseptic (sore throat remedy) and I was surprised to see that it was either 30% or 3%( I can't remember) but the main medical ingredient was phenol I wonder if you can do a extraction?
I guess iron ion in Hemoglobin acts as catalyst when it comes into contact with hydrogen peroxide and Luminol mixture.
I am in UK and we don't have super iron out so what products would beable to use instead
Nice video!Thanks
Doing a lab report without doing the exp so I’m glad I get to see what I could’ve done
The forbidden snowcone topping
Excellent !!!!
How du you find enough time to do all go this...
Cant we use sodium sulfite or sodium thiosulfate in place of sodium dithionite
@NileRed great interesting video, maybe i'll try it myself..... something else i wanted. You're using mainly magnetic stirer that means you don't have to have boiling stones but from my experience it's better to have boiling stones. Mainly for destilations and methods where is required lot of heat. My magnetic stir hotplate is not efficent as yours because of that i'm using old ETA spiral hotplate (slovakian thing) , it's capable of much higher temperatures (sometimes really crazy) as my magnetic stirer. Well in that case i need boiling stones. At school we're using broken porcelain plates but i personaly prefer ceramic filtration rolls from aquarium filter because they don't react and have much more surface than porcelain fragments. Thanks that i can use much higher temperatures without bobbling out.
I made this in OChem Lab, and it smells strongly like tomato sauce (like cheap spaghetti). Don't know how else to explain this. Do you know why this is?
Hi Nile, maybe a weird question but where did you get your vacume filter system from?
Using this for my lab practical in 2 hours. 🤩🙏
what are we going to do with this? Is this to check to make sure you washed all the blood away? just asking.
Hi, please, if it's possible, wouldn't you like to make a video about how to get an iron from the blood?
What was the mole of Acetic acid.
Would it be possible to use 70% concentration lag grade Acetic acid.
Next up: Synthesis of fluoroantimonic acid! Please.
Ain't that the most corrosive acid in the world?
+MrMatnor2403 yup.
You're going to need a lot of teflon. Good luck.
Teflon: I'm ready baby!
15:53 what? Random australian?
*new zealander
Way too nasal to be a kiwi!
i never get tired of seeing things change color and get vacuum filtered 0-o
I don't care about the science. Im just here to see Wizard Nile mix stuff around and brew potions
Can one use FeSO4 or FeCl3 instead of K3Fe(CN)6? The latter is fairly expensive.
I can't understand shit about chemestry but I still enjoy watching Nile's videos
What is the source of hydrogen in the reduction of the nitro group to an amino group?
Not that palladium on carbon is easy to come by, but in theory, you could have used hydrazine sulfate to reduce the nitro group as well, but as I've never run such reactions in glycerol, I can't say for certain if this could be converted to a 1 pot process.
Some quick questions about this synthesis:
1) Why did you use acetate to free the hydrazine? Wouldn't hydrazine (bp 114 C) boil off before the acid (bp 119 C) does? Chloride seems like it would accomplish the same goal, except it is even easier to obtain and has a lower boiling point. It also seems like the acetic acid and hydrazine might have potentially reacted to acetylhydrazide, hurting your yield.
2) Why was the nitro reduction done after the hydrazide was formed? The basic conditions are capable of hydrolysing hydrazides.
I'm no chemist, but what I do know from other endeavors is that having your thermometer directly in contact with your beaker during heating might not be the best idea.
It has to introduce some variance due to the thermal nature of the glass. I'd think you would want the business end of the thermometer in contact with nothing but the solution you're monitoring.
I guess since there is a temp. range in this reaction it isn't that critical... But I've found that using best practices habitually ends up being better in the long run.
TFW you go to watch a video making luminol, then suddenly realize you've stumbled upon the Chemical Avengers entry in some weird chemistry extended universe and now have to watch a dozen other videos to see what you missed.
So I’m deciding to try to make luminol for my science project. *Boy, I don’t know what I’ve just done.*
NileRed working with hydrazine: "We got some splashing, but that's okay."
7:49 why does the caption of this step say "Added 0.2g ammonium carbonate" if he is mixing distilled water into the beaker?
Notice some videos mentioned that you’ve since deleted from your channel. Have you considered uploading them to somewhere other than CZcams?
What other commonly available reducing agent I can use to reduce 3-nitrophthalhydrazide? I don"t want to bother with making sodium dithionite (I could if no option)or buying sodium metabisulfite- NurRage or thiourea dioxide like UC235. Can I in this case reduce NO2 group in other way like for example in aniline preparation buy Zn and HCl or anything else?
Me too. Fe/HCl is also used. My concern is the hydrolysis of the hydrazide functional group....
Just realised the channels NileRed and NurdRage can both be abbreviated to NR, I found it a funny coincidence.
Thanks!
Have a coffee
"it becomes clearly opaque"