Determination of Ash Content (Total Minerals)_A Complete Procedure (AOAC 942.05)
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- čas přidán 1. 07. 2020
- Determination of Ash is one of the important proximate analysis for food, feed, vegetable and many other samples. It represents a part of quality of a sample. This method removes all kind of organic matters from a sample which may interfere in other chemical analysis, for example: analysis of heavy metals, minerals.
Moreover, this video represents a complete procedure for determination of Ash from a food sample which will be very helpful for a laboratory analyst.
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I'm student of food science and technology and this video helps me alot. Thank you
Thank you so much
I'm also a student of food science nd technology ☝️🤗
I am a student of food science and technology, this video is really helpful found it just at the right time .
Am in Food Science and Technology
@@awololarukayat4481 what's your next plans ...can you guide me because I have same subjects?
You videos ARE EDITED very nicely sir. and are also very helpful👌
Thanks and welcome. Stay with us.
So informative, i have discovered your channel and I become addicted to your videos... The music is suitable with the video.. Also you describe the protocols in detailed and simple way.... Thank you
Glad you like my channel. We will continue and also improve with your suggestions. May I know, where are you from?
Thank you very very much....I am a biotechnology student ....I learn most important concepts from your channel....thanks a lot...keep doing such video for students....thank you
Rajesh Tayade, you are most welcome. Many important videos are still waiting to be uploaded for you. Just be with us.
@@MicroChemsExperiments thanks a lot sir
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All lab test R very Easily define💙
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8 oct 2021
Thank you. Stay with us
appreciated the work keep it up
every video is a big help. ❤️🙏 SALAMAT
I'm so glad to hear this
This has been really helpful, thanks
Thanks
nice explanition.really interesting thank you!!!
You are most welcome.
Thanks for sharing this...its very helpfull
Thanks
This videos are very useful for me sir. Please upload more practical videos sir. Thank you 🙏
We will. Please mention the test parameter if you have any suggestion.
Clear concept,,, good job❤️
Thanks
❤❤ awesome and great demonstration
Thanks
Very informative .... bhtreen
Thanks
This Vedio very helpful. tq so much
@Selvakumar, its pleasure...
Thank you for being with us.
Perfect experiment and Nice explain
Thank you so much
amazing vedio thanks for helping for preparative practical in examination.
Helpful vedio
Thank you. Stay with us
@@MicroChemsExperiments Ok
Thank you so much sir for this video.
Most welcome
ur video very nice..very easy to understand👌
Thank you. Keep supporting us.
This video is very helpful. Easy to understand due to step by step explanations. Thank u
Anees Hayat, you are most welcome. Be with us.
Thank u soo much sir u r way of explanation is excellent alot of love from Pakistan ♥️♥️
Thank you so much. Stay with us.
@@MicroChemsExperiments yes surè 😊
Very helpful 👍
Thnkkuu soo much
Most welcome 😊
Thank you, sir.
You are most welcome
This is excellent. Thank you Very much💥
@Mohammad Shahdat Hossain, you are most welcome. Thanks for being with us.
Thanks very helpful
Abdulla Al Rifat, you are most welcome. Be with us.
Very important
Thank you for teaching me
It's my pleasure
I would like to ask if my sample is fish feed pellet. Do I have to grind my pellets into powder form first for the sample preparation?
Thank you🎉
You’re welcome 😊
Helpful video
Thanks
Very informative!! Thank you so much, your video helped us in our class thesis. I hope you can see this and reply .... how can we burn our biomass briquette with a crucible ??? thank you
Make the biomass briquette ground and in power form. Then take the powder of you sample in crucible and do the experiment as described in this video.
Thank you so much for staying with us.
Wonderful
Thank you
Good job
Thanks
Thank you sir
You are most welcome.
hello sir, what is the alternative way of testing ash content of briquettes without the use of muffle furnace?
Good... thanku
Most welcome. Stay with us.
عاشة الايادي
Thank you.
Why do you have to wait for the oven to drop below 250C before taking out the crucibles? Thanks!
Fire insident will occure if you open the furnace at a temperature higher than 250 degree celsius.
Thank for the nice video. I have some questions. My samples are not turning to grey color. I keep it 48 h is the muffle (600 oC) but it still has a light green and reddish color. The ash content is about 30 %. I think it may be too high. My samples are the herb extracts with different concentration of ethanol. I don't know what wrong with my sample or procedure. Hopefully, you see my comment and kindly give me some advice.
Keep burning for 5 hours. Few samples are remained green and reddish after ashing. Just ensure that the black color is removed completely.
Thank you for the illustrative video, may you share a link for the volatile matter determination procedure if you have one.
czcams.com/video/DtSH8n1jbyM/video.html
Thank u sir🙏
You are welcome
Awesome video! Question, I understand combustion reduces food to inorganic mineral ash. I’m confused, if the body uses minerals however the minerals become inorganic after digestion, how is it assimilated?
Spinoza Black, thanks for your question.
Inside a human body, digestive juice creates acidic condition in the stomach to digest you food to convert it into difderent micronutrients and thus releasing minerals. These are further absorbed into the blood vessels.
what exactly is the homogenous sample that you add in after heated crucible, may i know if the crucible is heated without any sample in it if thats the case then the sample is soil right
We used tea sample for testing in this video
4:37
Hi it's good but I think some informations are mismatched. In video At 4:37 you said 700 degree Celsius for 2 hours but you main heading at 5:02 show for 4 hours Pls clarify to clear any doubt or false information and pin me if I'm not wrong.
Rest seems fine and thanks for the information
Sir, Please add some papers (links of books etc. ) on why do we need ash content and how different samples have different values for it and more detailes like this. Tha'll be very helpful. Some reading materials.
You can get many related documents by searching Google. We will try to cover documents with this channel in future. Stay with us
@@MicroChemsExperiments Thank You.
we are performing ash test of honey sample so the standard valves of ash in honey is not more than 0.6 ,,,,i ask you that is ash percentage is depend upon the food sample?? is moisture is more supose 40 then what to do
Jalal Shah, Thank you fot your comment.
Ash% totally depends on the minerals & heavy metals content present in your honey sample. So high content of heavy metals & minerals means high Ash percent.
You can reduce Moisture from your sample by increasing the density of your sample. This can be accomplished by drying the sample at elevated temperature.
It is very good vidio for ash test thank you for prepering this educational vidios! Question! the final ash result after calculation is wet weight based or dry matter based ?
Wet weight based result
Would it be a problem when i place the crucible inside the furnace without covering it, as in my lab the crucibles do not have lids. Please assist
It is better to use lid to avoid the loss but you can carry out the analysis without using it
Thank you thank you
😂
You are very welcome
Is it the same procedure for the determination of plant extract
very nice , sir please make video on Determination of acid - insoluble Ash
Muhammad khan, Ok. we will work on it very soon. Stay with us.
Muhammad Khan, we made your desired video. Please take a look at....
czcams.com/video/JecqdeVUTpE/video.html
Good
Thank you
Tq❤
You are welcome
Very informative video..
I have a question does we consider Sodium (Na) left in our product as Ash After drying??
Yes.
Hi there! Sorry, which edition of AOAC is this method from?
AOAC 19th Edition
Thnks
Preksha Srivastava, Welcome
I want to measure the ash content in plastic granules, but I have porcelain crucible without a lid. Is it fine if I use these or I have to purchase new ones?
Mia, In case of plastic granules you can use crucible without lid to determine the ash content as plastic content won't loss during incineration. But in this case, large size of crucible is more preferable to use to avoid sample loss.
Thank you very much for the immediate response! Also, very helpful video!
Mia, you are most welcome. Be with us.
is this methods specified for animal feed ash detection?
can we use this methods for spice also ?
Yes. You can
Hi, brilliant video, I'm doing this in high school and was wanting to know would processing bring about changes in sodium and potassium levels in the likes of raw beef?
No change is needed
Can we perform this ash determination for peanut bars powder????
its very nice ; l have one quations if you are willingness …how to know water absorption capacity for pasta dough by farinograph is possible or not…if you have a link please share it
I don't have any link
Interesting. This was informative. I have a question, hope you see this and reply. So what if I want to get the ash content from meat e.g beef? Do I blend it to homogenize? If I place in in a furnace, would it burnnnnnn, like burn burn that it sticks to the inside wall of that bowl?
Crucible *. Not bowl
@@tjakielo2968 You need to blend the beef sample first. Then take 2g sample for testing and follow this video strictly. You will find the ash content after the burning.
@@MicroChemsExperiments oh Thank you
@@tjakielo2968 You are welcome
Am not asking how to measure moisture content but I want to know before carrying out an ash analysis the sample may be high moisture so how it dried to prevent spattering during washing?
I mean ashing process
I got your point. Dry 10g of your sample in hot air oven at the same condition as described in Moisture content determination video and cool in desiccator. Then analyse the dried & cooled sample for ash.
Why you don't post any procedure for calcium and phosphorus in feed
I have a doubt regarding the closing of the crucible while heating. If the lid is closed will burning or combustion takes place properly? I think it has to be heated with the lid open.
No. The crucible must keep open while heating, according to AOAC method. When you will apply heat at above 550 degree Celsius, the sample will burn out, no matter the crucible is open or close. Crucible is kept closed to avoid loss of ash during burning.
@@MicroChemsExperiments thankyou..❤️
@@surajvvv121 Its pleasure
to determine the ash content of spice powders and wheat flour, semolina is it necessary to do charring or can we directly put the crucible inside the muffle furnace without doing charring?....please help!
Charring is better because it facilitate to become your sample carbon free very fast. You can analyze without charring and there will be no problm in your test result. But make sure that you sample becomes carbon free completely and no black particles is seen in the ash.
@@MicroChemsExperiments thank you for the response!... so after charring we need to immediately place it inside muffle furnace and not in dessicator? . Can we place it without the lid ?
@@poogylama1737 After charring, place the crucible inside the furnace immediately. Lid of crucible should be used to avoid loss of the ash content while burning inside the furnace.
Desiccator is used when ashing is done until taking the final weight.
Is there any problem if don't use crucible cap? I mean putting the crucible in muffle furnace without cap ?
It is better to use cap.
Do we need to make our Sample dry at 105°C for 1 hour before the ash determination?
No need. But you can incinerate your sample above 150 degree Celsius for 2 hour after taking sample weight and before going for the ashing into furnace
Hi there! If I want to analyse ash in liquid sample (milk), does this method applicable? Or is there any other suitable method? Thank you!
This method is applicable for liquid sample but one more step should be followed.
First of all, take your milk sample in the crucible and place on hot plate. Then heat the sample to dry it. After drying, follow the burning steps in furnace as described in this video.
@@MicroChemsExperiments Alright, thank you!
@@amninazifa5244 Welcome
hello, thanks for this information
i have question, if i want to analyze the ash from animal feed, is the method the same ? or have a difference ? thanks
This method is also applicable for animal feed sample. So, you can use it
oke, thank you
@@aasl6808 You are welcome. Stay with us
This is very helpful, but also I would like to see a video on how to do analysis of yeast and mold in solid foods.. thanks
Already working on that parameter. Stay with us
hello ,The video is very useful and how can i get the standard of ash test (AOAC 942 .05) i want to do ash test for chocolates , can i follow the same step in the video and crucible is with out lid can i use it? how many hours will take the furnace reach to 250 deg.c thank you.
waiting for your reply.
We could not provide Ash test documents. Plz follow this video for Ash test of chocolate. Crucible with lid is better than the crucible without lid. But you can use crucible without lid. An Ideal furnace will take only 2 min to reach 250 degree Celsius.
@@MicroChemsExperiments thank you for the reply one more question what is ideal cm need for metal tong ? 25 cm is ok ?
Can you share the link for buying those heat resistant gloves
You can find from local scientific markets
sir do we grind the sample in powder form before performing the experiment?
Yes
Hello sir, is the weight of the crucible have a constant value?
When you will dry a crucible enough, you will get a constant weight of that crucible.
Is the same method applicable for feedstocks of biodiesel?
Yup
Hi! Is this a suitable method to transform bone sample into ashes? Thanks
Not sure. You can try
Make a complete video on proximate composition.
Anees Hayat, We have already make complete videos for proximate parameters (Moisture%, Ash%, Protein%, Fat%, Fiber% etc.) Please find it out from our video list. Thanks for being with us.
@@MicroChemsExperiments ok thank u so much
Why didn't you burn crucible in preparation of crucible .doesn't it change in weight of crucible if it burns before adding sample?
You are right. Crucible should be burnt and cooled before taking sample in it. But we just dried in oven. Both can be done but burning is more appropriate.
Hello sir if you had made any video about acid oil test like tfm.miv
Thank you so much.
Can we keep the crucible in the furnace over night?
Yes. You can.
I'm getting confuse, makeing ash 2 hrs or 4 hrs . kindly explain me
Ashing is done for 2 hour at 700 degree Celsius. But if you burn the sample at lower than 700 degree Celsius then you need 4 hours of burning.
Please I need an analysis on the observation urgently
Your question is not clear to me. Please rephrase it
sir i have a doubt , how can it be that the weight of the crucilble after heating was increased which should have been decreased because moisture and co2 would have been lost from it till now . plz clear my this doubt
Weight of crucible increased because we are subtracting empty crucible weight from crucible containing sample which is ashed.So after heating there will be ash+crucible weight
What is methods of active matters testing of Hand Wash/Dish Washing Liquid please explain..??
Noted the test parameter. We will develop method for it soon. Stay with us.
@@MicroChemsExperiments Thanks
prepare video on Gumms content in soya crude oil
Shrikrushna Chaudhari, we already uploaded GUM content video. Link: czcams.com/video/syTwwvQAA5I/video.html
@@MicroChemsExperiments thanks
What is the factor for other grains like for maize, sorghum, etc ❓
What factor are you talking about? Please clarify
Do we need a mositure free sample for ash content?
It is not necessary to make a sample free from moisture before ash analysis. Moisture indicates a quality of sample. So analyze your raw sample for ash content determination.
Hello, please can you download the method for this experiment in writing
If you need the soft copy of this method, I can provide you. Please knock to our facebook page.
@@MicroChemsExperiments ok 😇
Why u r not using H2SO4 what use on it ?
This is only Ash determination. So we dont need to use H2SO4 in this test.
If you looking for Acid Insoluble Ash test, please find from our video list.
How can I test a plant to see if it's an acid or a base
use pH meter
is it applicable to honey?
I did but honey ash turned to black color.is it right?
This is applicable for honey but the ash should not be in black colour. Burn again after adding 5 ml ethanol
This is actually good but try to provide theoretical knowledge behind the experiments...
Great idea
What sample u can taken that experiment?
Tea
What is diff. between ROI and sulphated ash?
Take help from Google. Thanks
Vitamin test of chakki atta procedure video din
Which vitamin test do you want??
Hello Sir can we calculate ash percentage of herbal oil and herbal gel plz tell me sir
Same method
Ok sir tysm
can i use this method for milk?
No. After taking Milk sample in crucible, it should be heated to dried up first. The. Transfer into furnace and do the same as in video
What sample did you take?
Feed
Can l replace the air oven with microwave?
No. You must use Hot Air Oven
How can we find the composition of ash?
Analyze ash to find heavy metals and minerals