Transcriptome analysis. Learn library preparation and data analysis from scratch.

[@MultiXyzzs]

Thanks a lot for this video. Useful, clear and detailed explanation. Please never stop uploading!

[@XploreBio]

Thanks. More to come!

[@ranahaiderali4821]

University lecturer can't explain this in 1 hour what you did in few mins.
Thank you

[@sangitabhandawat842]

Excellent video abhishek, keep it up

[@snehasishchakraborty3167]

Excellent video on transcriptomics.need more video like that.

[@harishmangesh995]

Thank you for this very informative video.

[@publiczero3684]

Very nicely explained

[@jyotipathak5939]

Please make some videos on genome data analysis.. also how to plot different graphs from the transcriptome analysis like kegg, COG, GO etc.

[@Aemilindore]

Thanks

[@mitrabinda1992]

Discuss some application of transcriptome

[@praveengugulothu7770]

super explanation

[@grsbiosciences]

Thanks sir

[@tilthesoup8107]

Thanks for the really nice video, which helps me to have the big picture of RNA seq workflow. I am a bit confused about the difference between mapping/alignment and assembly. Is the difference between their outputs only the length of the sequence?

[@XploreBio]

Assembly: Joining of shorter sequences to get a large sequence.
Mapping: is aligning sequences to reference sequence(s). It gives you information about sequence/read/gene abundance/expression.

[@ashwiniashwini7494]

Can you please explain what is unigenes, contig, and transcripts and difference between them in transcriptome analysis

[@XploreBio]

I have already uploaded the video..

[@jahnabigogoi3288]

Please explain what is unigene and difference between unigene and transcript.

[@XploreBio]

Will be uploaded soon

[@jahnabigogoi3288]

@@XploreBio Thank you so much! Waiting for more of your videos. Awesome explanation

[@Humble258]

U should consider explaining in hindi ..that would help many students😊

[@XploreBio]

I would love to do that. But for global audience, I need to stick to English

[@xuhangwu1770]

Is there any example of using Nanopore sequencing for transcriptome sequencing?

[@XploreBio]

Thanks for asking.
You may refer: www.ncbi.nlm.nih.gov/pmc/articles/PMC5553090/

[@tiyasaguha5413]

Can we use this for lncRNA as well?

[@XploreBio]

We ca mine lncRNA from transcriptome sequencing also. There might be dedicated kits for lncRNA sequencing but I am not aware of it.

[@sadullaabdullaev5451]

Thank you a lot what about if you will work with us?

[@XploreBio]

Thanks for writing. What kind?

[@sadullaabdullaev5451]

@@XploreBio seedlessness of grpae

[@nilaypatel3690]

Explain slowly