Thanks for the really nice video, which helps me to have the big picture of RNA seq workflow. I am a bit confused about the difference between mapping/alignment and assembly. Is the difference between their outputs only the length of the sequence?
Assembly: Joining of shorter sequences to get a large sequence. Mapping: is aligning sequences to reference sequence(s). It gives you information about sequence/read/gene abundance/expression.
Thanks a lot for this video. Useful, clear and detailed explanation. Please never stop uploading!
Thanks. More to come!
University lecturer can't explain this in 1 hour what you did in few mins.
Thank you
Excellent video abhishek, keep it up
Excellent video on transcriptomics.need more video like that.
Thank you for this very informative video.
Very nicely explained
Please make some videos on genome data analysis.. also how to plot different graphs from the transcriptome analysis like kegg, COG, GO etc.
Thanks
Discuss some application of transcriptome
super explanation
Thanks sir
Thanks for the really nice video, which helps me to have the big picture of RNA seq workflow. I am a bit confused about the difference between mapping/alignment and assembly. Is the difference between their outputs only the length of the sequence?
Assembly: Joining of shorter sequences to get a large sequence.
Mapping: is aligning sequences to reference sequence(s). It gives you information about sequence/read/gene abundance/expression.
Can you please explain what is unigenes, contig, and transcripts and difference between them in transcriptome analysis
I have already uploaded the video..
Please explain what is unigene and difference between unigene and transcript.
Will be uploaded soon
@@XploreBio Thank you so much! Waiting for more of your videos. Awesome explanation
U should consider explaining in hindi ..that would help many students😊
I would love to do that. But for global audience, I need to stick to English
Is there any example of using Nanopore sequencing for transcriptome sequencing?
Thanks for asking.
You may refer: www.ncbi.nlm.nih.gov/pmc/articles/PMC5553090/
Can we use this for lncRNA as well?
We ca mine lncRNA from transcriptome sequencing also. There might be dedicated kits for lncRNA sequencing but I am not aware of it.
Thank you a lot what about if you will work with us?
Thanks for writing. What kind?
@@XploreBio seedlessness of grpae
Explain slowly