Salting in and salting out | How does salting out happen? | what is salting in of proteins?

This video explains the concept behind salting in and salting out in details but within 5 minutes. this video will answer following questions
What is meant by salting out?
How does salting out happen?
What is salting out in protein separation?

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This is the best video for explaining the concept of Salting in and Salting out.... Thankyou dada for this....l would like to ask you about the reference where l can get the exact text of this explanation!!!!!

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There is no 1 proper source for it ....several review articles and text books . But you can follow voet and voet

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I usually have a hard time understanding how some of these processes work but you did an amazing job at explaining the process! I feel like I understand it and could explain how it works to another individual now. Thank you!

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[@SuperPuddingFish]

Short and clear, thank you!

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[@theanabolicscientist]

Beautifully explained thank you! I've been struggling with sample preparation for months now - I have VERY expensive triple-labelled samples for NMR so, naturally, sample stability has been a great concern to ensure we can get the most out of each prep.
Have you got any rough kind of buffer optimisation "workflow" that you go through when determining protein solubility? I've thus far tried crude hanging drop precipitate screens using a crystallographers microscope, as well as a thermal denaturation assay utilising a hydrophobic residue-binding dye which infers protein unfolding using qPCR (ThermoFluor I believe is the technique name).
Have you got any other suggestions? I'm still struggling somewhat with long term stability.

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[@ducktape8520]

Great video! Just have a litte remark:
At 1:52 you mark the carboxylate ion which is part of the protein peptide backbone. Instead you should have marked the carboxylate at the amino acid side chain which is exposed to the enviroment.

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[@shubhamsaha6130]

Thanks ! I was looking for salting out mechanism in saponification. I hope the mechanism will be similar, as both protein and soap are polar. Thank you !!

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Best explanation for salting in and out. I want to cite this reference
Haha. Thank you so much

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​@animatedbiologywitharpan
I am really interested in your video!
Could you let me know more about the detailed mechanism of the Salting-in step? From your video, I understand that adding salt ions can make the structure of the protein stable along with reducing of the intermolecular replusion of the protein. Then, what is the mechanism of the water molecular can form the hydrate shell around by the protein?
Is hydrogen bonding on the hydrophilic regions of the protein?
Thank you!!!!

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