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Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Thank you! However, in future videos, maybe it would be better if you could add some possible controls for the technique. e.g. having only beads as a control to identify non-specific binding.
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
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So if a protein A interacts with protein B but we need to know if it is subunit B1 or B2, can we use the same method by doing two pull down assays ( one with protein A and B1 and one with protein A and B2)? Or is there another technique for that?
Really glad to know it was useful. Please share my channel with friends. You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Your videos are really very interesting and beneficial especially your Drosophila related. Are u working on Drosophila? as during my summer training I watched your videos for basics.
Hiii everybody ! I made a pull down essay but I dont understand how I am suppose to present the result and how I have to read it. In the video its explain really quickly in the end in 2:10 min. Someone can explain to me ? I made input WB, Pull down WB and Pull down Coomassie
Make a correction in your video bro: GST is not part of plasmids, plasmids contain genes of interest, not proteins of interest. Genes of interest then encode proteins, here the GST works as bait. GST is added, not encoded by the plasmids as you have shown in your diagram.
GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this vector is a GST-tagged fusion protein in which the functional GST protein (26 kDa) is fused to the N-terminus of the recombinant protein. Because GST folds rapidly into a stable and highly soluble protein upon translation, inclusion of the GST tag often promotes greater expression and solubility of recombinant proteins than expression without the tag. In addition, GST-tagged fusion proteins can be purified or detected based on the ability of GST (an enzyme) to bind its substrate, glutathione (GSH).
@@animatedbiologywitharpan agreed bro, still, you need to use word ''GST,s DNA sequence'' as you you mentioned in your reply. But in video, maybe unconsciously, you used the word GST Protein in plasmid. As you know the sequence will translate into GST protein.
Well done !
This is very, very good.
I didn't expect it to be this good.
Thx mate
welcome .... I am glad that it helped you..
Thank you, this is so clearly explained I understands how this works now
So helpful - God bless you.
Thank you very much. This was concise and extremely understandable. I appreciate it!
Glad that it helped you.....share among friends and help them as well
You teach very well, and give unique informations that are important too. Thanks!
Thanks Deepika.....
Great job explaining it so well and making it so easy to understand! Keep it up :)
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This helped! A big thanks :)
Thank you so much!
i am Definitely giving a thumbs up. This is simple and clear. Thank you
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This is so good!!!! SAVE MY LIFE
You are a hero.
Speed and perfecly clear!
Francesco Silvestro please share among your friends and help me to reach big audience
you have explained it so clearly. Thanks.
Please share my channel link with your friends and help me to reach big audiance.
Really very nice video.........
Thanks for your efforts that you made.....
Appreciable
Hemant Goyal share among your friends
You just explained what my prof couldn't/wouldn't -_- Thank you!
Glad to hear that you liked it
Please share among your friends and help me to reach big audience
excellent!! Thanks a million!
If you like the explanation please share among your friends and help me to grow
I always watch all your adds.. that's my guru dakshina 😊
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me . The suppurt startds from as low as 40 rs.
Thank you🙏
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Thank you! However, in future videos, maybe it would be better if you could add some possible controls for the technique. e.g. having only beads as a control to identify non-specific binding.
ya i just wanted to keep it as simple as possible ....
Good explanation , i am french and i have understand with any difficult
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
Massive thanks for your amazing job ! You are actually better than French biology professor... ;)
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So if a protein A interacts with protein B but we need to know if it is subunit B1 or B2, can we use the same method by doing two pull down assays ( one with protein A and B1 and one with protein A and B2)? Or is there another technique for that?
Excellent. I didn't expect that I'll understand within 2.30 minutes 😅
Really glad to know it was useful. Please share my channel with friends. You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Thanks
please share with friends
thanks man!
Please share with your friends and help me to reach big audience
omg thanks so much!
Please share among friends and help me to reach big audience
great video
xiBoggy thanks ,that keeps me motivated
Your videos are really very interesting and beneficial especially your Drosophila related. Are u working on Drosophila? as during my summer training I watched your videos for basics.
shivangani lohani yup I work with drosophila neural circuitry
Arpan Parichha Ty for all Drosophila related videos as I will be doing my dissertation in this field only.
shivangani lohani oh nice..... you can keep my whatsapp no 9051392160....if you need some help.Where you want to do desertation??
omfg thank you, hopefull i wil pass my exam
Please share among your friends and help me to reach big audience
Hiii everybody ! I made a pull down essay but I dont understand how I am suppose to present the result and how I have to read it. In the video its explain really quickly in the end in 2:10 min. Someone can explain to me ? I made input WB, Pull down WB and Pull down Coomassie
Great video for research students
Good to know you liked it
THANK YOUUUU
Glad that my video helped you
Please share among friends and help them as well
this video is good and helpful but it's to fast, make it a bit slower
shezote talukder these videos are made in terms of global standards..... always you can play the video in 2x speed to slow down
Make a correction in your video bro: GST is not part of plasmids, plasmids contain genes of interest, not proteins of interest. Genes of interest then encode proteins, here the GST works as bait. GST is added, not encoded by the plasmids as you have shown in your diagram.
GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this vector is a GST-tagged fusion protein in which the functional GST protein (26 kDa) is fused to the N-terminus of the recombinant protein.
Because GST folds rapidly into a stable and highly soluble protein upon translation, inclusion of the GST tag often promotes greater expression and solubility of recombinant proteins than expression without the tag. In addition, GST-tagged fusion proteins can be purified or detected based on the ability of GST (an enzyme) to bind its substrate, glutathione (GSH).
@@animatedbiologywitharpan agreed bro, still, you need to use word ''GST,s DNA sequence'' as you you mentioned in your reply. But in video, maybe unconsciously, you used the word GST Protein in plasmid. As you know the sequence will translate into GST protein.
Thanks
Most welcome! Please share with your friends to help my channel grow...😇