Primary Cell culture and cell line | Cell culture basics
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- čas přidán 5. 07. 2024
- In this video we would discuss the basics of primary cell culture and try to look at its application.
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Hi Arpan, Excellent video!!!! I am going to use it for my MSc students. Great to talk you you last week and to know about your channel.
Thanks Bhaskar da, all the presentations are available in case you need let me know
wow....Your lecture is perfect. Thank you so much! (from KOREA)
Thanks a lot....please share with your friends and help me to reach big audience....and lots of love from India
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Korea 2
Thank you for making this easier. Keep up the good job.
outstanding video and such an informative lecture of cell culture. thanks
I LOOVE your animations and the way you explain all the techniques. Very useful for us master or PhD students!
Thanks for sharing this content.
Thanks a lot for your compliments.....please check out my other playlists as well. Lots of love from India 🇮🇳
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Nice video, I do have a question, you said before that cell lines have limited life span and then on the table it says cell lines have unlimited lifespan, is there an error somewhere?
This was so great! Thank you so much!
you say at 3:30 that cells with highest capacity of growth predominate - does that mean as you create more subcultures, you will get more and more of a single cell type (being the cell type with highest growth rate)?
I hereby appreciate the way you explained animal cell culturing, and especially hippocampal culturing and widely used organotypic culturing. Thank you for this informative lecture! I've loved it. :)
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No explanation in the world can be better than this!!!!
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Really thank you so much for this video..i was reading a paper about immortalized astroglial culture and wasnt able to understand properly...ur video literally gave me a clear base line...thnks once again
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
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Can we get video on techniques used for single-cell culture?
Let me tell you this is the best video i have ever seen. I mean you are literally so great. The way i understand ufffff.
Thank you so much
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Thank you so much 🙏🙏❤️
Appreciate your efforts 💯
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your video is well organized...explained a lot...thank u very much...
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thanks for this helpful video :)) It seems a crazy future awaits us
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Omgggg!!!! The real images of hippocampal primary culture are crazyyyyyy!!!!
Good to hear from you ....stay tuned and keep watching
thank you arpan, it helped me alot :!! can't wait for ur next video
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Thank you sir!
I really like this video! Nice drawings!! 🥰🤗
Greetings from Germany ☺️
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Thanks a lot for the beautiful video.
you help me in doing my lab report. 😁👏🏻👏🏻♥️
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wooow,Thank you for making it easy
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Thankyou so much again...me being a m.sc in chemistry...now doing a phd in chemical biology ...i need to know these basic terms. Reading does help but animated videos are just so awesome.
And your videos help me a lot.
Really glad to know that my contents are helpful for colleagues like you in other discipline. Please feel free to connect via email.
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@@animatedbiologywitharpan sure.
...im yet to explore other videos from your channel. If i dont find them, i would request you to make a video on them 😂
Please feel free to suggest
@@animatedbiologywitharpan like RNA modifications and their role in antibiotic resistance as well as cancer, phage resistance and ways in which viruses evolve to finish that resistance.
😇
very easily elaborated contant for basic understanding
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Very nice nd unique.... thanks
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just to confirm, following the passaging of the cell and centrifuge for cell suspension, the cell is then left in the media to grow?
Ya very little amount of cell from the suspension is transferred in a new plate containing media for further growth
Bro I appreciate the effort your lectures are very organized pretty and well done. Also, you’re good in explaining complex ideas or subject in such simple way. Thank u so much for everything you’re a hero to all of us.
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Best video so far
Don't forget to share with friends
Live savior indeed thanks
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Excellent!
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Thank you!
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You are really pro 🙏
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sir i have a question that a normal cell line will die after how many division?
Generally after 7-10 passage it should not be used for experiments....cells might accumulate many mutations and undergo telomere shortening...but this is highly variable for different cell lines
How can I make that media n serum for vito meat. U have the video sir?
Sorry bro 8 Don't have a video on that
hello arpan , your videos are really good. can you please suggest me some good but simple books for cell culture
You can reffer to Wilson and Walker book but honestly its a practical thing so we learn it once we do it
Hi..this vedio helped me a lot ..
Can u pls let me know where can i buy head and neck cancer cell lines (specially tongue) in india at reasonable price
I don't know it
Thanku sir for this video
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if we make cultured for endothelial cells these cells may not accurately reflect organ specific endothelial cell characteristics what is the reason ? ist related to aberrant in gene expression or what happen ?
Very nice question , in reality extracellular matrix plays important role 8n tissue morphogenesis and cell fate specification . If we use isolated cultures, all those external signaling will be absent and hence fate specification could be altered . It's always a problem for cultures
@@animatedbiologywitharpan Thank you very much
Zabardast 👍
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Very good explanation sir🥰👌
Thanks for the compliment. Please share my channel link with your classmates.
Don't forget to checkout all my playlists.
Can you explain me the 8:17
Hi , is it possible to produce whole liver from a single liver(hepatic)cell ?
Yes now a days scientists have developed liver organoids ( mini liver ) check it out www.frontiersin.org/articles/10.3389/fmed.2021.574047/full
@@animatedbiologywitharpan thank you for providing the link ,I just read the Article in which they using genome editing, tissue engineering technology,chip based technology and after reading the article I am more keen to study more about these technologies ,thanks!
@@rashikasingh7607 I work on same technology….but I build brains…feel free to reach out at arpan.tifr@gmail.com
@@animatedbiologywitharpan nice,I am a MSc.biotech student I also wanna do some interesting research or build some helpful techniques for us ,will definately do.. thanks for motivation.
Good to hear from you….stay tuned
Sir can u refer books to learn cell culture techniques
Honestly there is no good book for learning cell culture....you have to hit the lab to learn about it
Slt
Svp esq vous pouvez m'envoyer le protocole de la culture cellulaire des hépatocyes!!?
great work
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Thanks Namrata for your compliments
👍👍👍👍
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what mean PBS
Oo wow
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Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Can you share us the bibliography please? 🤗
"Saludos" from Mexico
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@@animatedbiologywitharpan of course, thanks a lot.
Primary cell the same with primary cell culture?
Yes
Tees aren't available in India?
It would be available soon via vistaprint....vistaprint has offered a sponsorship ...we would bring these merchandise at very reasonable cost ( bellow 500)
what is mean by cyhrid
I will give the answer in a different video
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@@animatedbiologywitharpan okay
@@animatedbiologywitharpan upload all videos only in english....im from south india...
What is PBS solution
Phosphate-buffered saline (PBS)
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make sure to checkout entire cell culture playlist czcams.com/play/PLKtiwIJ8Q7rpNrZRxzBgoH2Q6oz4bJwg2.html&si=wryecbGL3FEk4uon
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Thank u so much... 🙏 Nice Animation. @@animatedbiologywitharpan
How are cell lines immortalized??
Immortalized cell lines are derived from a variety of sources that have chromosomal abnormalities or mutations that permit them to continually divide, such as tumors. Because immortalized cells continuously divide, they eventually fill up the dish or flask in which they are growing.
can u share notes?
It's chargeable $5
the sound is not good I am quite disappointed
Sorry about the sound...
I'm sorry but I just cannot get through the language barrier. While I'm trying to comprehend one sentence, I'm missing part of the next, and so on! I see by others' comments, they could...I'm happy for them...
I am so sorry to hear that
That fake accent though😂😂
u can teach in Hindi... don't try to be a foreigner it looks awkward if you are not eligible enough
I want my content to reach a broad audiance. Any way thanks for your feedback
@@animatedbiologywitharpan good thinking but u can do better in Hindi
nice video
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