Streak Plate Method - Amrita University

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The most common method of separating bacteria cell on agar surface to obtain isolated colonies is the streak method to inoculate Petri dish. It provides a simple and a raid method of diluting the sample by mechanical means. As the loop is stretched across the agar surface more and more bacteria are separated until the required bacteria are deposited on the agar. After inoculation, the area at the beginning of the streak pattern with show confluent growth while the area at the end of the pattern should show the discreet colonies.

[@triforcejj]

Great video: very clean, organized and easy to understand. Thank you!

[@sanjidaislam1]

i was having trouble getting isolated colonies..yeah..this video sure helped..

[@TheSternSaver]

The lack of isolated colonies on the agar plates in this video show that this is not a good demonstration of proper streaking technique.

[@MrVisho0o]

amazing video...thank u alot...u made microbiology to easy and interesting to learn :)

[@theophanyc.asogwa4375]

i really can't thank the author enough.......need i say? it was very helpful "to me"...yeaaa

[@annadagmarapl]

thank you so much for the video..very helpfull

[@prerana32]

very well explained...........loved it.thnk u

[@deepsundar100]

this video help us very much,........thanks

[@rasheenanimhani3315]

this is very useful for students

[@rasheenanimhani3315]

thank you very much

[@tanyadutta7850]

it is a very informative video

[@amrabano2048]

thank u vry much

[@chandu17abhi]

nice .. very useful

[@jimjutte]

Thanks very much. I was looking for something simple and to the point for my high school student, but this could easily by used as a quick review for first year university students too.

[@NazirAhmadMalaknad]

Here Liquid mean liquid media which contains salts and source of C and energy, like TSB

[@hpribot]

Yes, under most circumstances. The aerobes will grow on the surface and any anaerobes that may have been present may show growth under the agar. I'm guessing you streaked to hard on the plate and dug into the agar, yes? You'll get your technique down, don't worry. BTW, some agar plates are firmer or softer than others, learn what to expect when working with various agar plates. Also, there are many, many types of agar plate formulae but usually you'll only be working with a few of them.

[@YablonskaOksanaSevama]

thanks! this video is very fine! agar is very dense, which concentration of it?

[@monirayusuf30]

thanks

[@trinbr]

really nice video i enjoy mirco labs :) its one of my favorites subject and this video is the best that i have found in streaking technique
thanx

[@khaliddahab9198]

nice

[@ismielhossen9063]

good method

[@mahesh9028]

@mermaidmelody123123 the bacterial colony is mixed into saline solution......

[@justthislee]

The flame is blue, but you flame sterilized the inoculating loop, so the flame became red.

[@superdebster2010]

i streaked my agar plate to hard and it separated will my bacteria still grow? please help!

[@bong1991]

anybody know what the main source of error in streaking for isolation of pure colonies?

[@yunqie]

I gt 1 colony from the plate and find out tat colony is gram negative bacteria, does it means that all the colonies in that plate r gram negative?

[@nadaa7295]

chai cindy no it doesn't.

[@dreamer6366]

how come the fire isn't blue?

[@hpribot]

I would think the streaking techque is incorrect. The point of streaking is reduction of the organisms via several streaks. Each successive streak having fewer and fewer organisms. Hopefully, you'll be able to isolate individual colonies and work with them.

[@janellacruz8124]

So it doesn't matter how many times you dilute it as long as you get isolated colonies?

[@lightningshark67]

That is correct, it does not matter how many times you dilute as long as you are able to obtain isolated colonies. As the purpose of a streak plate is to separate the bacterium into individual colonies. (For further inoculation, inspection or just to identify different types, etc. )

[@EduardoDiaz87]

EXCELENTE VIDEO...AMO MAS LA MICROBIOLOGIA

[@britneythao]

What do you mean "separated?" Do you mean you break the agar? All the bacteria need is the components from the agar. So, I think the bacteria still grew, but you might not have had good isolation, did you?

[@MissKatAttackOfficial]

It would be easier to keep the agar dish on a surface and barely lift the lid while streaking

[@goitsekamoletoaba2099]

if it broke it wont grow well

[@hpribot]

I conditionally disagree with you. Read my reply to Debra Tumboe.