Structural Studies of Phycobiliproteins from Spirulina - Amrita University

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Spirulina is a photosynthetic organism which is considered to be a promising source of proteins, is taken as the source of proteins for studying the structural changes that occur due to denaturation of proteins. It has a photosynthetic membrane which has a light absorbing part called a phycobilisome.
Phycobiliproteins contain a covalently attached chromophore, phycocyanobilin, which is constrained in a planar conformation by the folded protein. Upon denaturation of the protein, the chromophore undergoes a conformational change, leading to a significant alteration of the absorption spectrum. By measuring the absorbance at 625 nm as a function of urea concentration, the free energy of unfolding can be determined.