Spectrophotometric Determination of Phycobiliprotein Content | Protocol Preview

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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis - a 2 minute Preview of the Experimental Protocol
Tomáš Zavřel, Dominik Chmelík, Maria A. Sinetova, Jan Červený
Czech Academy of Sciences, Department of Adaptive Biotechnologies, Global Change Research Institute; Masaryk University, Department of Plant Physiology, Faculty of Science; Russian Academy of Sciences, Laboratory of Intracellular Regulation, Institute of Plant Physiology;
Here, we present a protocol to quantitatively determine phycobiliprotein content in the cyanobacterium Synechocystis using a spectrophotometric method. The extraction procedure was also successfully applied to other cyanobacteria and algae strains; however, due to variations in pigment absorption spectra, it is necessary to test the spectrophotometric equations for each strain individually.
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