Agar Transfers/ Sectoring Off Healthiest & Strongest Growth
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- čas přidán 8. 08. 2024
- Hey Myco Family! In this video we go over some basics of Agar work, transferring / sectoring off the healthiest growth on your plates to continue on your cultures. These basic principles can be applied to cleaning up your cultures in the case of bacterias or contamination.
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patreon coming down the line!
Do like I do. If I find a video that I like , I just download it. Make my own archive 😁
I would love to see more in depth agar work!!!!
Great videos bro, I know how much work it takes to film your passion. Thanks for all the work.
I've learnt sso much from you brother! Like a full on PhD in mycology.
I'm in the planning for a more commercial sized set up with a walk in enclosure with racking either side floor to ceiling with totes to try a couple different spore variety. Very exciting
😄
Careful on the quantity your growing. Depending on your state you could get a lot of years in the can.
I'm about to setup a greenhouse lab in my house once I get my custom hood from labRatz, I took this hobby on when I got clean of meth after 15 yrs and it's saving my life! I watch you pgt mycophilia, mycodawgs and also Garry from fungi perfect...you guys have this down to a science, that's exactly where I want myself in the next couple years. Plus I'm teaching my kids homesteading :) anyways thank you for taking the time to teach us students 😊
LabRatz is the shit I have the nomad lyte I love it
Thanks for the detailed process!
I want to get on Patreon and support you, really had fun and learned alot!
Hit you yesterday in // to get on it! I’ll be the first sub brotha! 🤘🏼
Great video ! Thanks
Love the agar work. Great video admire your work.
"Chrisfix" of mushroom cultivation. Great videos man.
Thank you brother
@@MycoTrophic When to expect your Agar making video?
@@delarois I’ll be making one here in the next few days
I am following your tek verbatim. I hope you have the pouring of plates done for me to watch after this. I have my first tub in fruiting. I used bucket tek. I like how you explain things and I rarely have a question that is not answered by the end.
Thank you. Mush On Brudah
This was a great video compared to others I've seen. We need various plates that contain contamination and show us how to see what's contamination and what's not. Some are easy to spot if it starts growing from the edge of the lid, but I have some plates that are sus and it's growing out with the mycelium and hard to tell if it's contamination or just a weak section of mycelium. I yet have to find a video that shows that specifically!
Thank you for the information...
Good job bro
loved the vid
Great info
Great video as always. I really liked the intro of the video, the patterns are used in the Peruvian Amazon culture Shipibo
Very nicely done sir. Precisely how i do my agar work. i didnt see you clean your receiving plate however. I always clean both plates and I'm sure you may as well.
thanks for the vids! how many times do you transfer or clone until you go back to multispore? thanks again. :)
i usually place my wedges mycelium-side down, have you experimented with this and determined that mycelium-side up is best?
Thanks
Good shit! After PC, is it ok for there to still be a little moisture in the jars?
Awsome
Any particular reason you dont put the transfer piece face down? Bet it takes a few days to crawl down to the new agar
I just knew, when you were talking about labeling the plate, that the label would rub off.
Good stuff! New here. How long, give or take do the dishes last ?
What grain did you add to the agar? Looking good in the fungihood 🤣👌🏼🖤🔥
Just the tip, that's what she said
If I took a wedge from 2 different cultivars, would I be crossing my own new variety? Or do I do that with 2 different spores?
No.23 scalpel blades 🙃 give ‘‘em a go
What do you do with the first plate you took a sample from? Do you throw it away? What to do with first initial plates, can anyone tell me?
What do you do with the original plate?
Can you share a link for the tray you use in front of your flow hood please. I'm using the top or an old rabbit cage and it's huge
I have that same scalpel
Where do you get your unique strains
Is my agar always going to be contaminated on the first dish when going from spore swab to dish? Or can I ise the first dish?
What agar recipe do you use?
I have 2 agar plates that have just the slightest blue tint on them on a little section is that normal have you seen that before?
Mycelium contains psilocybin. If your cultivating a psychoactive mushroom of course. The tint of blue could be a bruised area causing psilocybin to oxidize
Whats do you call that rack you use to put the agar plates on, trying to find one but dont know what to look up
Cabinet organizer/shelf. Box stores carry them.
Do you use food coloring to get black agar plates?
Activated charcoal
I would
Do you sell prepoured plates?
Would support you
How many transfer do you normally do until puttin it into grain 😊
depends on the variety, but generally 1-3
Can we get a update on this bad boi?
Where can i get this white small stand from?🙏
Yeah I was thinking the same 😂
Where can I get one of those racks to work on? What is it called?
Cabinet organizer. Your local box store.
Can you cross 2 monocultures?
3:45 you don't wear an N95 during culture work?
I'm just curious, I've taken samples of strong tomentose and strong rhizo and they both seem to grow at the same rate. Am I doing something wrong. What's the best characteristic to sample
Just keep transfering til u have even growth all the way around. I like to take small thick strands in early transfers.
Also u can use a light under ur plate to help, but this guy knows his stuff. Food coloring ftw.
Hey man where did you get the rack from?
I've found similar ones from thrift stores (goodwill etc.) I have a stainless one I got second hand.
where did you get this iso lmao. ik trinity is magicmyco
I got it from a friend
If you create a Patreon acct I would be happy to support...
Do you incubate these at a certain temp?
70-74F
@@MycoTrophic I've heard that this range of temperature (70-74F) is used for fruiting conditions and not incubation. I've seen people setting the temperature to 75-76F for incubation, considering that there is a rise of temperature inside the cake (although its an agar plate). Whats your opinion about that? Thanks for all efforts!
@@lucasantonini7618 essentially the higher the temps the higher risk of contams. I get amazing results with 70-74 (72 is the sweet spot)
@@MycoTrophic cool! I'll tray this temperature too! Big hug!!
Yea cubes are forgiving with temp.
Why everybody placing transfered agar with mycilium upwards?
I’ve tested finish times for this, both were the same
@@MycoTrophic Then if there is no difference, why everybody still places it stricktly upwards?
@@xojioghbiujust easier maybe?
🌈💎
@MycoTrophic hey! If you have a good flow box lead rn lmk . Alot of place are out of stock and I'm shopping around for a 2'x4' one 🙏❤️
If my petri dish is contaminated, should I transfer 2 or 3 times and then I'm ready to use this mycelium to do an inoculation? If I use the mycelium after 1 transfer just, there is risk of contaminating my grains? Thanks!
You only need to transfer until you have no visible contamination. Sometimes it only takes one transfer to save or clean up a culture.
@@MycoTrophic Thank you man! Just saw your answer right now that I come to see again the video.. I didn't have checked to receive comment's answers lol
"This is the real work out of the whole process."
🙀DUUUUUUUDE........
YOU ARE NOT EXAGGERATING! I'm fucking sitting in essentially a damn hazmat suit when it's 90°F in my damn apt for some stupid reason and i gotta stay in the suit to be locked in a closet that's sterile so i can OH SO CAREFULLY!
Open up the healthy agar inside an enclosed air box that's with in this sealed closest so i can do this transfer without contamination being a factor (hopefully) and on some real shit man, this phase really is the most tedious and fucking sweaty. 😵
Man it really does make a difference tho! AGAR ALL THE WAY!
👁✊✌🤘🤙👁
Cut a star out of your mycelium and place that in your new dish...that'd be kinda sick 😅
Давай субтитры на русском,мы тебя тут тоже смотрим
This was true a week ago. I seen the time lapse video of sections taken from different places on the same plate. The weakest section was the fastest strongest growth. Humans are so sure of things until they aren’t. Mush love to all🫀