The Agar Files - Making Transfers on Agar
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- čas přidán 26. 12. 2020
- Welcome to The Agar Files, My series for learning agar work in mycology. Today's class is going over making transfers on agar and we'll be doing a little cleanup in todays work.
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I don't do anything with mycology but randomly got your broke boi tek video recommended and binged this channel since. I have OCD and mild aspergers and get stressed to hell when following instructions/ "how to's" in general but something about this is just so calming on a visceral level. Thx man.
You should try some mushrooms as a therapy and report back
I agree, very cool and chill dude here.
@Aco Znas94I ...psychotic..??
Jordan Babcock Psychotic mushrooms are what you hallucinate on a bad trip? 😝
Heya. Wondered if you’d like a tip (which to be fair you might already know).
Get a chill tune with a swaying beat (kind of like the background music here) and learn your instructions to the tune. Then when you carry out the instructions move to the beat and it’s like you’re dancing them out.
It’s very calming and helps both my neurological spasms disappear and my partner’s Aspergers concentration levels heighten and he de-stresses.
Also what others have said regarding psilocybin (microdosing) helps Aspergers stress for people I know but it’s not for everyone. Food for thought though. Literally.
Make the instructions into a song and the movements into a dance. It becomes graceful and natural and calming.
Very few channels can explain this craft right as a whole. From spore, agar, fruiting conditions and more. Even less can actually teach. Thank you for being one of the rare gems that can explain in a way many can learn. One hell of teacher. A golden standard of one if you will lmao. Mush love ✌
Instead of throwing out the one with a small spot, do the transfers then put a little h2oo on the spot then cut it out. I have also poured h2oo into the agar up to the the mycelium, wait for the bubbling to stop and pour it off. It pours off the contam with it. Have saved many agar plates like this and grown good mushrooms.
you mean hydrogen peroxide ?
@@SaranshKarira yes
Can u record a video?
what percentage do you use? will 3% do? how about 30%?
@@ankanahumko8486 probably 3%, 30% is crazy strong and would eat through the agar itself
Litterally just put some agar in the pressure cooker and saw this. Perfect timing
Perfect every time
I'm pouring my first agar in about 15 minutes. Thank you for all the videos!
When the student is ready the master will appear. 🤟🏽👽❤♾🛸
Love the video and love this comment! I am ready then cause I have found this channel.
@@alltaira5922 same! It’s all about timing ⏱🧫🧬🍄🔥🖤
Yes 💯 !! I'm here I'm ready !!🤩 Synchronization ✨
Transferring clean, snow white mycelium onto a plate of virgin agar is one of the things I enjoy most about working with mycelium. Waiting for your LC video now...thanks for helping all of us improve our skills, PGT!
What is the point of that I don’t get it I understand the ones that were contaminated but the clean one why would you want to transfer it?
@@jroc09 To isolate a certain growth.
What would be the benefits of that
@@jroc09 In mycology, there‘s ‚contam(ination)‘, and ‚iso(lation)‘. Isolating a certain growth from a clean culture can provide certain characteristics that you want to isolate, e.g. especially strong growth for fast colonization of substrate.
Im doing transfers off my first agar plates today. Much appreciation for all the info. Mush love. 🍄
Can you please do a video demonstrating how to create/store an agar "library" ? LOVE your content sending peace and love
@PhillyGoldenTeacher I'd like to see this too! 🡹
#metoo
Thirded
I was just wondering about this
I used to watch the X files. Then I discovered the Agar files. Thanks so much, PGT 🤓✌🏽
I love your videos man, they are always great quality 🎥
Love watching agar work 👏
i feel like im watching some professional video on the television. i was so excited for this video!!! long live your channel bro. youre a legend
What a great video! I appreciate all of your hard work and making it easy for us!
you're an amazing person. thank you
Just inoculated some grain jars, and I just gotta say Teacher....I have always been Interested in the thought of trying to do this myself, and it was YOUR videos that gave me the final push to actually continue and start to teach myself about my new journey in mycology! I'm completely green...and im looking forward to making the many learning mistakes I have in my short future🤣 but I just wanted to thank you for all your efforts, in making all these AWESOME videos that have taught so many like me to just stop worrying and GET INTO IT! I'm trying to not be like so many who rush and make newbie mistakes along the way with dreams of how fast and easy their first flush will be🤣 but we will see how I do...excitedly watching 10 jars of b+ on brown rice grains slowly start to turn fuzzy! I bought spores instead of a liquid culture syringe though...so I've already made my first mistake😂 sorry for the long comment, but I just needed to thank and tell you that YOU'RE the MAN! And NOW? here I am learning about agar from you! absolutely making a difference in the world my man! Mush love
Really
How has you Mycology journey been?
Be prepared to fail. That's not me being horrid.
I'll be doing it soon thanks to this video. The clear closeups were very helpful.
Great video. Really have come a long way in the short amount of time you have been on youtube. Really looking forward to the genetics videos.
Thanks for all the amazing videos you make growing mushrooms very fun and entertaining. I’ll try to grow my very own. Greetings from México
Not only are rhizomorphic mycelium quicker to colonization of substrate but they are also more likely to create pining/fruits.
Not true homie
Its all about the conditions you have to trigger pinning.
Dont misinform homie
Mush love, keep up the great videos
Love your videos man
Top notch as always mate
You're a god among men for these tutorials thank you so much
Just discovered your channel. So relaxing. Thank you for the info and the vibes
well looks like i found out what im doing today!
Thanks for the update.
Thanks for this PGT
Love you pgt mush love 🤙🏽🍄❤️
Casual flash of the enigma label, been trying for enigma for a while now! Awesome videos!
😁🍄
If u put ur cups in ur oven when ur pouring ur agar an putting the lids on in the oven will cut down on contam .350 works great to blows the contam away ,
From mush love ❤️
The Astro way is a great channel too ,
Love ur style great job on ur channel an ur teaching nods like me 👍
I really need a new hobby and this is great. Can't wait to grow some lion's mane.
So glad I found your page mush love
If you have left over pennicilin you can extract it and put it into your agar to prevent contamination
ALL HAIL THE GOLDEN TEACHER!
ALLLLL HAILLL!
Great tool ...thank you..
ThAnks phillyyyyy
This is mega informative.
You the GOAT bruh
Yesss 🍄
PGT is my fav shroomer!
I did my transfer just this past saturday. Unfortunately this video didnt come out in time for help. But all of them are doing GREAT! nice and healthy and fluffy white myc. Got some Golden Teachers and some Tidal Wave
How do you get those flavors? Could use some spores
Where do you get the spire prints from? Also liquid culture syringes?
@@carinapalomino8069 you cant get a liquid culture syringe but you can get a spore syringe as for where to buy then go to reddit or shroomery.
I'm doing GT right now as well, is your GT mycelium fluffy on your agar like mine is?
@@sheeeitmayn4384 its been a while but yeah i had fluffy growth.
TY for the VIDS 😀
Great vid thanks...
ALSO THANK YOU FOR THE SHIRT! I ♥️IT..
Glad you like it!
Your awesome ❤️💯🤘🏻
PGT…You da’ man ☮️☮️👌👍❤️
😁🍄
Thanks
Great video PGT. Once an agar cup is fully colonized, how do you store it and for how long is it good for?
@10:00 'Oh-oop" myco talk for - hope that side of the cup was sterile :-) Love your funny and educational videos sensei!
Pgt! I love your channel, you’ve helped me and all of us tremendously thank you! I have a question. I followed your agar recipe, and transferred some agar from another plate just like you did in this video, and almost 2 weeks later I still have no growth in them. I think I might have not boiled the mixture long enough or I might have put too much food coloring. Would either of those be to blame?
Nice
Hey teach great videos very interesting I’m new to the agar my question is this once you transfer to a clean plate do you just throw out the old ones even if there’s no bacterial growth
Nice video! Quick question, how would you store extra unused sterilized grain? I made to much and I was wondering how I should store it to keep it for a long time?
When making an agar to agar transfer, does it matter whether the transfer is placed agar side up or agar side down?
I am subscribed, how does one get to the discourse community. Outstanding video, PGT. Mush appreciated.
Dig the new intro
Thank you
🙏
Great vid, what kind of cups are those?
i cant waint to try
Hi PGT. Always love your work. Thank you!
Quick one: when transferring a slice of culture from one dish to another, I notice some people put down the small wedge upside down in the second agar dish. Others put down the wedge right side up. Does it matter, and why?
Kind regards, and thanks in advance.
Great videos! Question about Agar plates... Have you ever tried to sterilize your Agar with the microwave oven? I have had some luck with this method, but haven't heard of anyone doing this.
That small spot looked like stray mycelium.... they usually happen when there is water dripping onto your spores ill get runoff that starts mycelium on the sides of the cup, but they eventually converge and form rhizomorphic mycelium!
From one tiny piece... Mushrooms are truely miraculous !
Really need me a flowhood. I wanna try these cups but to worried of contaminants lol. I just stick to no pour tek till I get one
0:19 the top corner growth is not mycelium? Also, once you make the transfer from cup 1 to cup 2, do you discard cup 2 or can use that for grain transfer as well?
Hey PGT, how do you store your agar dishes if you make them before youre ready use them?
Love your videos, I hope that you talk about your insight on microdosing if you have any. Keep it up!
I notice that you don't use petri dishes covered with parafilm. Would that disposable container you use still be fine? I feel like air might get inside even when they are closed since they are made of thin plastics.
Thank you for your precious postings
What's this? A youtuber with affiliated links in the description that actually tells you they make a commission off of them? I'm thrilled! Love your vids.
Just wondering, do the lids keep out contamination as well as petri with parafilm?
Very new to this about to try and run agar do you leave the portion cups or Petri dishes inside the still air box after inoculating the agar
Hey, what do you usually do with the leftover agar with mycelium?
Would storing the agar cups upside down reduce the condensation and also limit contamination?
🙏🙏🙏
Curious about timing. Total newbie here-sorry if it’s basic... Does a spore to grain take about the same time as a spore to agar in terms of mycelium development and growth? My guess is spore to agar works faster?
Question! Do you sterilize your agar solution and then pour into the disposable ramekins? Or do you sterilize the agar and ramekins together like in the pastywhyte tek? I assume the ramekins would melt in a PC but you never know 🤷🏻♂️
Is that the methylene blue you have in your agar? I've never used it. Fish antibiotics from the pet store make for great antibiotic agar.
Late for class don’t have the materials list or myc. Someday soon I hope.
Ayyyy, do you let your poured agar sit for about 5 days or so to ensure it wasn't the agar? I'm JUST starting and there's sooo much controversy of letting it sit vs using it soon
If you made the agar pots and don't have a hood would it be ok to spray some iso alcohol on it to kill bacteria and leave for storage? (With lid on)..
one last thing I'm going to try some clove oil in my agar mix would that work... thanks
If you take a culture from an apparently healthy dish do you throw out the original dish with the healthy culture or keep to take more samples from?
Do you pressure cook those PP5 containers? I tried, lot of melting!
Could you put this leftover in your yard and it possibly grow mushrooms in the wild so to speak
Amazing! Thank you for the excellent videos man!!!!!
Also, do you know why peptone smells so bad? Literally probly one of the worst things I've ever smelt. Bacteria in rice jars has made me puke. But that peptone poop smell is something else. 😆
😄🍄
Im learning here . Is it possible to cut off a bit of mycelium thats just colonised an all in 1 grow bag and put it in a new all in 1 bag to colonised that ?
Enigma plate---Show off !
I would be very interested in a liquid culture video.
Just search for Mossy Creek Mushrooms here on YT. They go over LC in detail on their channel.
It's in the works!
All I'm gonna say is stir bars, don't forget the stir bars.
@@AaronAlso in a few months I'll mess with them. For now I just throw in a marble for my LC jars. Stir bar is a luxury for little old broke boi.
@@PhillyGoldenTeacher
Oh! gee, back in my heyday (on "the forums") when I was in college I would have sent you a couple stir bars. You can get a basic kit on Amazon these days for ~$50 with plate, bars, and extractor.
My only problems with LC have been ignoring gas exchange and stir bars; or these days forgetting the stir bars.
I really admire you passing this knowledge on to a new generation of amateur mycologists; my hat's off to you sir.
PGT- do you sterilize your agar cups? What’s your method?
Sensei! A Question: After multiple transfers were able to get rid of contam from spores, mycel stops growing after about a week of growth (size of dollar coins roughly). Multiple transfers, same problem. Is it moisture? Heat?
No contam visible, it just...stops.
Can anyone offer advice?
The Joe Pera of growing mushrooms
where do you keep you agar containers?
What’s your sterilization process for ketchup cups ?:)
Should 5he mycelium be Fury white? I have a plate that I put a small pin 📌stem and cap in and there are 2 round but different types growing
Can you made Temella fungi tissue culture.
How long do I wait to use the scalpel/needle after sterilizing with fire. I don’t want the metal heat to kill the mycelium
That last agar dish that you did the transfers from can you still reuse that
How early can I transfer mycelium from a spore swab to agar? I have some specks that are in the infancy of colonizing my agar dish can I transfer the myco that looks promising I have a few strains of Rizo growth but these are small about the thickness of a nickel just little strings the growth on some specks are smaller than a pencil eraser in circumference I’m trying to catch the fast growing myco early by cutting out the spots of rapid growth compared to the rest of the dish before it all combines I guess how early is too early?
awesome thanks, are you walter?
Hello! Thank you! Where do you get your blades/scalpels? Also for your last agar dish you transferred from, do you through that away after? Even though that last one didn’t have contamination?
Those are exacto knives...you can get them anywhere. Amazon, hobby stores etc.
How long does a agar plate usually take to completely colonize? I inoculated played 2 weeks ago and they seem like they’re barely colonizing. They’re growing in very thin. Is it bad if I just let the agar sit there to see if it’ll colonize more ? I don’t have any contam & I’m just confused at this point. Could it possibly be bad genetics? I’m a amateur btw only 2-3 months into this hobby 😅
Should I put the mycelium up or down against the agar plate?