Making and Using Agar (for mycology)
Vložit
- čas přidán 14. 09. 2021
- CZcams deleted my old channel, this new channel will only have less controversial videos. To get FREE access to ALL CONTENT and a free spore print, please use patreon, I also post and interact with people there: / gordotek
Before asking a question, please read the FAQ: / 22774475
Social Media Links:
/ gordo.tek.33
/ tekgordo
In this video I show you how to make and use agar. Written up TEK is here: / 56209839
Recommended LMEA recipe:
8 grams of light malt extract
8 grams of agar powder
400ml hot water
[Note: If you use any of my Amazon links it helps support the channel! (“As an Amazon Associate I earn from qualifying purchases.”) I make zero from CZcams. Thanks!]
Petri dishes: amzn.to/2Xgvauu
Inoculation loops: amzn.to/3CcX1uB
Xacto Knife: amzn.to/3ltxYwv
Premixed MEA powder (not recommended but for the lazy): amzn.to/3AbcTNF
To make your own mix:
Light Malt Extract: amzn.to/3k9ao8Q
Agar Agar Powder: amzn.to/3AdiJ14
Lighters for flame sterilization: amzn.to/397qmu8
Good value blender
amzn.to/2Dsq5QR
Blendtec Blender
amzn.to/2XjhBdu
Large pressure cooker (great value and good reviews):
amzn.to/2Fsbfgr
or smaller one: amzn.to/399EX8n
Kitchen Scale (great reviews, inexpensive)
amzn.to/2BobRP0
Large plastic funnel with strainer insert: amzn.to/2RDBzeQ
Optional parafilm: amzn.to/3CcZhC9
But ziplock bags work fine: amzn.to/3lp70Go
Optional stuff only if making PDA or black agar:
Honey: amzn.to/3k880zm
Activated Carbon: amzn.to/3EkTufx
Music Credit to Gil Scott Heron - The Revolution Will Not Be Televised
• Gil Scott Heron - The ...
"The thing that's going to change people is something that no-one will ever be able to capture on film, it will just be something you see and all of the sudden you realize I'm on the wrong page..." -Gil Scott Heron (explaining the meaning of his song)
Don't forget to follow me on Patreon and get your free spore print. My next video will NOT be on CZcams. Recipe cards from this video can be found here: / 56209839 - Věda a technologie
CZcams deleted my old channel, this new channel will only have less controversial videos. To get FREE access to ALL CONTENT and a free spore print, please use patreon, I also post and interact with people there: www.patreon.com/GordoTEK
Before asking a question, please read the FAQ: www.patreon.com/posts/22774475
facebook.com/gordo.tek.33
twitter.com/TekGordo
In this video I show you how to make and use agar. Recommended LMEA recipe:
8 grams of light malt extract
8 grams of agar powder
400ml hot water
The following links support the channel!
Petri dishes: amzn.to/2Xgvauu
Inoculation loops: amzn.to/3CcX1uB
Xacto Knife: amzn.to/3ltxYwv
Premixed MEA powder (not recommended but for the lazy): amzn.to/3AbcTNF
To make your own mix:
Light Malt Extract: amzn.to/3k9ao8Q
Agar Agar Powder: amzn.to/3AdiJ14
Lighters for flame sterilization: amzn.to/397qmu8
Good value blender
amzn.to/2Dsq5QR
Blendtec Blender
amzn.to/2XjhBdu
Large pressure cooker (great value and good reviews):
amzn.to/2Fsbfgr
or smaller one: amzn.to/399EX8n
Kitchen Scale (great reviews, inexpensive)
amzn.to/2BobRP0
Large plastic funnel with strainer insert: amzn.to/2RDBzeQ
Optional parafilm: amzn.to/3CcZhC9
But ziplock bags work fine: amzn.to/3lp70Go
Optional stuff only if making PDA or black agar:
Honey: amzn.to/3k880zm
Activated Carbon: amzn.to/3EkTufx
Music Credit to Gil Scott Heron - The Revolution Will Not Be Televised
czcams.com/video/QnJFhuOWgXg/video.html
"The thing that's going to change people is something that no-one will ever be able to capture on film, it will just be something you see and all of the sudden you realize I'm on the wrong page..." -Gil Scott Heron (explaining the meaning of his song)
Don't forget to follow me on Patreon and get your free spore print. My next video will NOT be on CZcams.
Thank you Gordo! Hey everybody, SIGN UP TO BE A PATRON! This is the most amazing thing, let's be smart and keep GordoTek going!
This is amazing, thank you so much. I'm new to this side of things and I REALLY want to get into it further (beyond just reading) but I'm having trouble getting spores since I dont have a credit/debit card at the moment.
I followed you here, sent a request on facebook and followed you on patreon (although I dont have the ability to become a patron just yet). I also plan on following your twitter if I can gain access back from whoever hacked it in the middle east.
Anyway, this was very helpful and I was wondering if there was any chance of being able to get a spore print/ spore syringe from you?
I'm trying to figure out how to request a spore print but idk how to use Patreon. I joined it and followed you but idk how to send a message
And folks…. You have to be at least a paid subber- which isn’t much to ask- to message and to receive the print, which also- isn’t very much to ask!! Considering all things! It costs him money and time to even create all these super amazing, informative vids, for his own self- so, it’s the least we can do to support him and his channel and work!! To help keep him and his brilliant work going, and able to be shared!! ❤ thanks for all you do! It means the world to me, that I do know for sure! Cause it’s content like yours, that’s literally built and shaped and created myself into the new myco lover I’ve become, transformed my own work in the hobby and community that I’ve now been working on, etc- all thanks to vids and content and knowledge sharing, such as yours!! So much appreciated Gordo!! Thanks for everything and I pray you’re able to always keep going and continue to share all of your advice, experience, years of wisdom and, vast amounts of incredible knowledge! Mush love friend ✌🏻🍄🫂💫💕
Man I’m so blessed to discover your channel before YT went dark.
Went dark?
@@Lurch-11 In the past, there was an abundance of informative content available, but nowadays, a significant portion of it is subject to strict censorship and gets flagged. Even stating something as obvious as water being wet can taken down.
Hey Is your dmt extraction video still up?
@@blueflame144 I believe it is on his Patreon.
Hey gordo I have watched plenty of videos on agar but the way you explain it and make it simple is awesome keep up the great work🙌🏼
Awesome, thank you!
Glad to see you back here on CZcams!! Love your content, and appreciate all you do!!
I am very thankful for all the effort you put in to help us beginners! I feel a lot more confident for my first grow because of your work. Much love from Oregon!
Thanks a ton. Just getting started with agar and understanding what I'm seeing after I get some growth is the hardest thing. Appreciate you taking the time to annotate your plat images and describe what you are seeing and why one this might be better or worse than something else. Thanks!
A little add on tip to this great video. If your town or city has a homebrew shop. They have many useful things for mycology. I homebrew and turned out I had everything but a pressure cooker and the agar already. Malt extract and yeast nutrients (dead yeast) as well as Erlenmeyer flasks. I was doing things like growing yeast cultures from bottled beer and such, many years before I got into mycology itself. It was a great knowledge base to have starting this hobby. Yeast is after all, a form of fungus.
The yeast is on our skin, we are symbiotic with yeasts. We sanitize and extract with their distilled excrement, It's the perfect fungi to start the mycological journey with.
Exactly. The LME at my local brew store is less than half the price of buying from Amazon.
Glad to see your posting videos again.. thank you sir
So glad I found your page, thanks for all you Gordo🙏🏾🍄
You are a wealth of information. Thank you for what you've given us.
Thank you for everything you do! Love these instructional videos!
Thank you for your help, Gordo! Your voice is so relaxing 😅
Blessed to see you again!
I noticed they removed another one of your videos. Thanks for your continued work!
Haven’t used agar, but I’ve been meaning to give it a try.
Excited for the cacti video!
Thank you so much for everything. The knowledge you share have accelerated my game beyond my imagination. Also, I admit that when I have trouble sleeping I put this video on. Your calm, soothing voice grounds me. Sorry if that’s weird.
Haha, believe it or not you are not the first person to tell me they like falling asleep to my voice ;) Someone wanted me to record some bedtime stories for them.
@@gordotekhaha that's hilarious and a little strange, but aren't we all? So, are you going to record the stories for me, eeek I mean them? 😂
Once there was a man finding a strange mushroom in a beautiful forest.... 😎
I've watched dozens of agar videos. This is best by far.
Nice, we love it when you post content. 👍 Can’t wait for those cacti videos 🌵
May the universe bless you! You have inspired me and I'm sure many others. Keep on!
Thank you for showing alternatives to having to order things online that may get you flagged! Much appreciated. It's also WONDERFUL that you allow your patreon to be viewed just by following, we poor people appreciate you.
I’m really glad I stumbled upon your channel. It’s now my favorite.
Glad you found it! ❤️. Some have said you don't find GordoTEK, GordoTEK finds you 😂
Gordo. Thank you for your work. Please dont be disheartened by youtube. Your video was how i started when my brother donated his BRF jars to me a number of years ago. I went on to find your videos after reading some books. Ive been highly interested in mycelium ever since and have even had a few successes trying to grow my own. There are a few players on yiutube who do an absolutely marvellous job of educating people of mushroom growing but to me you are without a doubt the real veteran when it comes to teaching. Please keep it coming and take a bow.
Thanks for the kind words! I'm pretty terrible at mastering the CZcams algorithm, I'm not what they look for, and I don't pump out constant content which is what they like. I don't really care, I'm perfectly fine living in relative obscurity 🙏😇
The legend is back!❤❤❤ Thank you so much Gordo🙏
#legalize psychedelics/ legalize our freedom/ legalize nature/ legalize spiritual connectedness
And human connectedness*
This is great! Thanks so much for creating such a thorough and informative video. You're an excellent scientist and teacher.
So much educational content on mycology is based on speculation and little science. It's great to see amateur science done right. It's clear you put a lot of effort into this video.
Love your work man!
Thank you for your enthusiasm.
Word up Gordo, thanks for uploading 🙂
Awesome. Thanks for making this video and posting :-))
Thanks Gordo im a year into myclogy with no sucess with agar with your guidance im thinking thats about to change for the better
Hands down the best to the point channel. Appreciate all your content. How can i get one of those spore prints?
Contact me on patreon or social media: www.patreon.com/posts/22774475
I can’t help but hear the voice in my head “Agar-agar will it blend?” “Yes it blends! (Don’t breathe this!)” -
Thank you for your useful video
OMG YOU HAD ME TILL ISO NOT BEING IMPORTANT LOL!!! Great video either way
I expect that reaction, but if you do the experiments yourself you will see that single plate isolation is just as good as 3, 4, or even 10 plate isolation. Try it out. Lots of myths in the mycology scene.
Thanks Homie
Thank you for all of your content, I have been learning a lot from you in the past 3-4 months!
I have a question though. I noticed you made multiple jars of the medium. Are you able to store it long-term and re-melt it if you want to use it again, or you just haven't shown in the video that you poured all of them in dishes?
You can re-melt (pressure cook) whenever you want.
@@gordotek you just made my agar work more convenient! 🙏 Thank you!
your the bomb .
thank you.
i appreciate you
Great choice of music!
A little intense 😅
⚫ activated carbon powder is badass. Very very easy to see any contam and makes it very easy to see the mycelium which in turn helps to select genetics
I still don't get the hype, I can easily see mycelium and contams without a black background. But if you like it, go for it.
Do you store them in the dark during incubation? It seems like some people incubate in open light
Oh I really appreciate this video and you executed it so well thank you very much sir I'll be watching many more of your videos I just hope that when I shut this page that when I open up your platform that there's more than just one video cuz that would bum me out a lot of them are like that
I've got lots of content that CZcams has banned, but its all still available and totally free (I don't use paywalls). Links are in my video descriptions.
When will the video of the free print cultivation be available on Patreon?
I know I've been working on it for far too long and need to wrap it up. At some point I decided I wanted to try a few more things before releasing the vid, which I did, that involved a lot more filming and I have a ridiculous mountain of footage to edit, but I've worked it all out and I'm very satisfied with it. I even have some very cool time lapse video, info on how to grow in a cold space during winter vs. in a hot room in Summer, etc. My goal is to have it published before the end of October. I will point out however that a very detailed write up of the TEK is already published on the patron page and it contains pretty much everything you need to know, I have updated it numerous times and refined it based on feedback and further experimentation. Many people have followed the TEK and nearly everyone who contacted me reported success including some who had never befoe grown any mushroom in their lives. There has also been nearly universal agreement that the species/strain was the best mushrooms they had ever experienced.
Love your content! Newbie here, preparing for my first attempt at growing. I couldn't figure out how to message on patreon but are you still giving spore prints?
There should be a message icon on the patreon app and on their website, put GordoTEK in the to box and I should get it. Or click on my name and then there should be a message creator link. And yes, I am still giving away free prints.
I love you gordo tek !
Would you suggest powder agar or agar agar strips. I know powder is easier to come by but if it's better I'd rather get the strip agar agar.
Thank you in advance.
I only buy the agar agar strips sold in Asian food markets but I don't think it really matters. I blend it up myself before using it.
Dude this was the easiest video to understand how to make agar agar to correct and simplest way.. My hate off to Gordo
I know this is an old video, but I’m new (lots of research but no grows yet.)
I’m now learning how to “breed” and copy genetics and have a lot of questions with not many answers yet.
For spore prints how are they stored? I’d like to be able to grow a good genetic mushrooms then a few years later replicate it, I know agar doesn’t last the longest, but would I be able to have a spore print for a few years? (At least if it’s refrigerated )
Spores should be stored at room temp, some species can last for many years, but they lose vigor over time. Other species don't seem to last very long at all, more like a year.
@@gordotek thank you, does agar keep for longer? Deciding between whether I inoculate or keep the agar for later
Does mycelial growth stay within the agar, or grow on top as well? Does it also reache upwards towards the top of the plate as well?
It will mostly stay on top of the agar, it can sometimes reach upward or try to get out of the plate depending on how aggressive the species is (Natalensis for example is quite aggressive, so is oyster mycelium).
Recipe 1 - Potato
3:24
Recipe 2 - LLE (recommended)
10:30
Why is LME recommended over the potato and honey? Does it colonize faster? Is it less prone to contam?
I compare them all in this vid, LME just produces the best growth with mushrooms (pretty much every species). You can also add a pinch of aged manure when working with dung lover species and that seems to speed growth a little too. But you can use the other recipes if you want, they will usually still get the job done.
To clarify once the agar has set, I can store it in my tent that stays between 76 and 80? I dont need to store them in the fridge?
Correct.
Question: Could I use the purple sweet potato and honey as a cultivating slurry to propagate oyster mushrooms? Leaving out the Agar. After pressure canning add a piece of pink oyster mushrooms. This is my first try and cloning the two I have in the fridge. Will this work to grow the mycelium?
I would search for something like "best liquid culture recipe" but yea I think it should probably work.
Isolab autoclavable pitchers ftw on pouring agar sir, poured tens of thousands of plates w. Em
Noted 😇👍
Is there a good video out there that explains all the different reasons you would use agar plates for mycology?
No sure, but basically your options are agar or spore syringe. Agar allows you to get some strain isolation and clean up the spores in case there is any contamination present. Spore syringe is faster but any contams in with the original spores are going to go right into your grain so you better hope the spores were super clean, and there is no strain isolation so many different genetics will all be competing against each other which can sometimes cause less predictable results.
can you make a vid on collecting spore prints and how to store them correctly...just and idea
Yes, I actually show how to do it in an already published video, here (jump to 18 minute mark): czcams.com/video/Z3OqlJB0D0Q/video.html I will show it again with a different species in my next video as well.
Recent sub here. Was wondering if you still have the spores of " The Crown Jewel" of mushrooms you speak of?
I used to give prints to anyone that asked but I've been overwhelmed and can't keep up anymore, sent out more than 1000 prints, so I had to cut back, but I STILL give free prints to all patrons at any tier (as little as $2 this basically just covers postage).
@@gordotek -- Great. Thanks for the information Gordo 🙂
you are awesome
I'll get on that sub if the prints are still available fam. Gotta restart my research and grow
I still send out prints to all patrons, as little as $2 which is below my cost. I just hope enough people stick around to pay it forward so to speak.
@@gordotek i will definitely do so. Just need to finish setting up my room for this. Need a a sanitary eork station agar all...i mean after all
agar is my favorite part of mycology by far
That's f-in' great!
What's the idea behind black agar anyway?
The idea is that it should be easier to see the white mycelium on top of a black background, but it didn't seem particularly useful to me, so why add the extra time and expense to make it? I have no trouble seeing mycelium on normal agar and kind of like how I can shine a light from the back of the plate right on through to illuminate the top of the plate.
@gordotek I agree. I don't even color my agar like some people, and I can see the mycelium just fine.
That's interesting though.
Thanks for the info.
@@m3m437 Same, the color thing seemed kind of gimicky to me too, I've even seen people color theirs green and red, those are common colors of contamination! No, don't do that, haha.
So if you have a spore print instead of a needle, how do you get started?
This video shows you how, and my (banned) bulk mushroom grow video picks up where this one leaves off (that vid is available for free on the patreon page see link in vid description).
Thanks
Thanks 🍄🫡
You are hella dope✊🏾💯
Hey, I have a pressure cooker but it doesn't have a pressure gauge. The manual says that the highest pressure mode is 12psi. How should I proceed?
Add an hour or so to your cook time. Same thing applies for instapot types of pc also. Tried, tested, and approved by yours truly ⚡⚒xXHeathenXx⚒⚡
Yea 12 psi is not so good, but if you add extra time it might work out OK. I would suggest looking for a better one if you can afford it.
Hello Gordo, I know this post is not for this, but I can't find any other way to communicate with you.
I would like to know how I can get Pancyan spore prints.
Could you help me please.
hug from Mexico.
Best way is to contact me by private message on patreon. But I'm also on all the major social media platforms.
Hello Usobrakets, the thing is you can get it anywhere online, @benjidelics store is one I know and have actually bought from him
Seek him on Instagram
Love you 😍
Not a single reason to be anything but proud of the outcome of this here video! Even the way you try to do a less annoying, more tolerable and less "h***cidal thought inducing" version of that super "pausy," inexplicable emphasis at the end of choppy sentences with what I picture would be ending a sentence with a '.' Then a space and another '.' Before continuing as though it's perfectly normal to punctuate your punctuation 😊
The how many plates does the recipe for agar that you prefer make?
A normal size plate takes 10-15ml of agar, and the recipe is 400ml so anywhere from 25 to 40 plates. I rarely make more than 10 plates at any given time, so often cut the recipe in half.
@@gordotek thank you for your time and help
I love you Gordo
💙
Any links to your parta?
Are Spore prints still available?
I give prints to any patron, its just $2 to join, so you can get the best genetics in the known world for $2, can't beat that.
How long did you let agar rest after pouring into petri dish?
Making and Using Agar: www.patreon.com/posts/56209839
They usually only take about 15 to 20 minutes to cool but leaving them in the laminar flow can also remove condensation from the lids which is good.
Carbon dust is highly explosive. Carbon+O²+ heat source =C0²
Indeed! ;)
shout out gordo!!!!!
Ove-Gloves rock btw.
Agree! ;)
How can i get free print , btw this video helps a lot especially for begginers like me
Contact me either on the patreon site or any social media (see vid description for links)
Went to your patreon and couldn't see anything about the free spore print..
You can contact me (private message) there or on any of the social media platforms (FB, IG, TWTR, RDIT).
Thank you for bid. Followed you on Patreon. Don’t see where to message for spore info.
There should be a message icon on the patreon app and on their website, put GordoTEK in the to box and I should get it or click/tap on my name and then there should be a message creator link. And yes, I am still giving away free prints.
No worries. I don’t see it.
Glucose can be used instead of honey ? I heard honey has anti-fungi properties.. ?
Probably, there are lots of recipes for agar out there, but simple LME seems to work best for most species.
In case you missed it, black agar is NOT recommended.
😂
You don't add any yeast to you LME?
No, but I have experimented with adding a pinch of aged manure and this does seem to speed up growth of pan cyan species anyway.
Don’t remember who said it, but there’s enough air in the dish already that they don’t need FAE.
Probably true, at least for the initial growth. I've never tried making it completely air tight, that would be a nice experiment. It might still grow, but perhaps a bit slower? You'd want to try it side by side with another that was not air tight to compare.
Hey quick question.... why do you think back agar is silly
I just don't think its very useful, I don't have any trouble using regular agar.
Please post your in depth how to grow unlimited mushroom video It taught me everything but I lost my book and can't remember steps :(
All vids (even the banned ones) are listed at the top of my FAQ and still available for free to everyone.
You are looking for "Bulk Mushroom Cultivation Video"
www.patreon.com/posts/22774475
Can replace the agar agar with rice noodles ???
No.
Using Ube potatoes?
Sad that CZcams (google, aka Alphabet inc.) is an American company and makes revenue from American taxpayers yet they do not hold American basic constitutional values. If you're doing business in America and making money from Americans I should be required to hold the most basic of American values. Other countries don't let companies do business inside their borders with our values so why do we put up with it... (Maybe because our laws are bought thru lobbiests....) I digress, thank you for content. I appreciate it.
Rehydration is not necessary from print to agar?
The spores will rehydrate on the agar which contains a lot of moisture.
@@gordotek thanks for the response!!!
I tried to follow you on Patreon like you said but I can’t?
The site/app is a little quirky, but from their own help page: "Log in to your Patreon account.
If you have the creator’s direct URL, you can plug that into your address bar, or you can search the creator’s page name in the search box.
Scroll down and click the Follow button directly under the creator’s membership offerings. When the bottom reads Following, it means you’ve successfully followed the page.
We’ll redirect you to the creator’s membership tiers page where you can become a paying patron if you’d like. If you’re not ready to join, you can exit the page." You can either use their app or their website.
I tried to message on patron but can't figure out 😢
I went to Patreon looking for the free spore print, I cannot find anyway to contact you!
Click the messages icon either on the app or website and enter GordoTEK
patreon.com/GordoTEK
@@gordotek I tried, it say no results found for GordoTek
i just saw this the spore still for grabs lol
Yea sending out "the champ"
@@gordotek how do i get my hands on them
❤❤❤
Why is it you don't recommend black agar?
Its helpful to be able to see the growth from both sides or shine a light from the back illuminating the front. I just don't think black agar helps in any way but if you like it, no biggie, go ahead.
How can i get a print
I still give free prints to all patrons, its as little as $2 and that's below my cost so I just hope enough people stick around for a little while to pay it forward so to speak.
Oh god, what is this used 4? Don't find a single word about that worth? I never ever heard of any of that; and I am really always on the lookout 4 news.
Mycology.
Ouh black smoke, don't breathe this!
Someone remembers?
What you know about gill scott heron💪💯
Real change occurs through active participation and not through passive consumption of media. Decrim movement is a good example.
I always Liked Charcoal Agar why dont you like it?
Just didn't seem particularly useful to me, so why add the extra time and expense to make it? I have no trouble seeing mycelium on normal agar and kind of like how I can shine a light from the back of the plate right on through to illuminate the top of the plate.
I’ve seen recipes that call for 200 gs of ME and agar with 2 gs of yeast I’m guessing that’s wayyy to much?
I guess that all depends on how much water is in the recipe, it can be scaled up or down but most people really don't need to make more than about 10 plates worth of agar for mycology work, so you really should only make when you plan to use immediately. You can easily make 30 plates even with just 16oz of agar, often I cut the recipe in half.