How to Set Up the PCR Reaction

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This video is the second part of a 3-part Getting started with PCR series that shows how to set up and run a PCR experiment.
The success and failure of any PCR experiment is dependent on preparation. This particular video shows the different components that go into a PCR reaction, how each component contributes to the experiment, and lastly, when to set up a master mix.

[@gowheregodgoes]

:( All I could think about, was the tip boxes are open the whole time.... Can't concentrate!

[@Tepmodify]

Thank you ...good vedeo.
When is you protect contamination.

[@lancelyde]

great video! I have a PCR lab tomorrow

[@user-sz5it4wm9f]

Very helpful video on PCR. Many many thanks for uploading this.

[@corbinmerrill1127]

Would this be the step that can be considered creating dilutions. I am doing a independent project for my school and it ahs to do with PCR and Electrophoresis. I am stumped on the Quantification, standardization, and dilutions part. I did this project a year ago and I am using it again but I do not have all of my data I used when doing this and it is bit of a headache.

[@kailashpatibhandari6561]

Nice..... informative and helpful video. Thanks

[@thermofisher]

Glad you enjoyed it! Thank you for watching.

[@iam3711]

best video on PCR, many thanks

[@thermofisher]

You're welcome! Thank YOU for watching.

[@coachjeremydraws4880]

Does that meant the template DNA was added to the tube?

[@thermofisher]

Thanks for your question Jeremy.
In a PCR reaction, template DNA is indeed added to the reaction tube.
For additional technical questions, please contact us at thermofisher.com/askaquestion.

[@setanta1966]

stopped short on stating NUMBERS of cycles used 🤔🤔

[@thermofisher]

Hi D Bond,
Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion for additional information. Thank you!

[@oyenimeapreala6460]

ThankS to DR Rorpopor Herbal on CZcams who helps me cured my PCR God bless you DR Rorpopor Herbal his herbal medication is very infective and active✨✨✨✨✨

[@ayashkair1641]

where can I do these experiments?❤

[@lancelyde]

in university level microbiology classes

[@nutribox4535]

in pathology labs

[@user-dg5sg7un7v]

I need translate to arbic😞

[@rimodotika4123]

why is she using glasse during manipulation ?

[@junbecks]

Depends on which university or institute you go to, they will have different requirements. Its not standard all over the world.

[@VladimirGent]

awful, sorry... it is for Chinese students in university , not for DIY-Bio amateurs.

[@sjdjjdaaasd3772]

what do you mean by chinese students?

[@Mirrori]

I'm only graduated laboratory technician and i could understand everything that's happening in this video. We did very similar things in school.