Quick Tips for PCR

In this video, you'll learn some important practical considerations and quick tips to keep in mind when preparing your PCR Mastermix.
This takes into account the same scenario presented in the PCR Calculations video: an experiment to clone the same gene from multiple bacterial strains where the Mastermix is prepared first and the strain specific template DNA will be added separately to each PCR tube later (this step not shown).
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The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada.