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How to Make Mushroom Culture Slants for Long Term Storage
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- čas přidán 8. 08. 2024
- In this video I show you how to make agar culture slants by allowing the agar to cool with the tube laying at an angle, resulting in a large surface area for spreading a culture.
Agar slants are better suited to maintain stock cultures due to the capability of the slant containers to be capped or sealed, preventing the slant from drying out while also preventing contaminants from entering the media. Slant cultures are also manageable to store due to their small size.
Some advantages over agar plates include less storage space used, less cell division, less drying out, and less chance of fruiting in storage.
Agar Recipe:
10 grams of LME + 10 grams of Agar Agar powder + 500 ml boiling water.
*Make sure to mix while hot and dissolve completely before pouring into the tubes. Make sure to work fast so that the agar does not solidify.
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Chapters:
0:00 Culture Slant Intro
0:24 Supplies
0:53 Popsicle Sticks
1:00 Jar Lids
1:12 Making Agar
6:12 Sterilization
7:23 Making The Slant
10:11 Verify Clean Slants
10:52 Inoculation
14:55 Colonization
15:10 Outro
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Music by:
Chillpeach - Mushroom's Life: • Video
Excellent..!!! This is the way knowledge should be given. Direct and simple. Thanks
Thank you! 🙏
There are only a few mycology channels that I find to be the most informative with practical demonstrations for the hobbyist. Yours is one. Thank you for making the time to think these through and share the details
Along with Philly golden teacher and 90 second mycology
@@chicofromph33nix64 I have not found them to be so. They are mostly focused on recreational psychoactives
I’ve always wondered why ppl use these over plates . This is pretty cool . May have to try it out !
You answered questions i had that i could not find in other videos . I like that you mention every part of the process including mentioning incubation , parafilm , lifespan etc . Well done and thank you
Thank you for watching and for the feedback! Glad it was helpful!
Thanks for the LC!!!!
Excellent video🍄
Thanks for watching! 🙏
Thanks it was informative
Thanks for watching! 🍄
Retired Semi-Pro Tip: For LOOONG term storage, a little mineral oil over the slant's colonized surface will prevent the culture from drying out.
I guess you should sterilise the mineral oil too?
Thank you 😊
Thanks for watching!
Thank you for this! It’s a game changer.
My question is : so I where I live I don’t have access to a lot of things. So for my agar recipe I use boiled potato water with agar.
Do you think it’s alright to boil the potato and the pop sticks together and mix agar into to that medium?
I think that would work fine!
You prefer the 50 ml slant over the 15 ml? I see you use both, you notice any difference between the two sizes
Is it best practice to go from an agar plate to slant instead of fruit body sample to slant, to identify contaminants?
Yes
Would you use the premade agar/supplement powder that you showed in your agar petri dish video for slants?
Yeah that works good
What molecule is your tattoo? Also great video!
Question: sorry if I missed the part but what temperature do you recommend for the refrigerator / long term storage? Great video👌
39 degrees F is good.
@@SporenSproutwithout fridge how much time a slant would last? (room temperature)
Hey dude! Sorry to no blasting your comment section but I need that tool you use to transfer agar wedges. What’s it called?
What is the popsicle stick for?
What is the type of malt extract used? Is it from local stores or laboratory grade?
Light malt extract from local brewery supply store or online here: amzn.to/4bB7Ict
Hey brother hope you are well
Badass content!
Mad luv
Peace out
Mad scientist organic grower✌️
Mush love brother
@@SporenSprout ahahaha well said, keep it up. Mad luv
whats the name of the tool used, to cut the wedges
Sterilized disposable scalpel is good to use.
Why use the water you boiled the sticks in and not use fresh water instead?
Just to save time, doesn’t really matter.
Why do people put popsicle sticks in there slants???
The mycelium will colonize the stick and if the mycelium on the surface dries up over a long period of time you will still be able to pull the stick out and put it on an agar plate to revive it.
How long can this slant stay sterile before inoculating
12 months or longer
My slants warped or became concave in the middle. Any clue why that would happen?
Didn’t loosen the caps enough to let gas escape maybe?
Why can’t I just load the tubes into the PC?
What is the purpose for adding the popsicle sticks?
Basically the mycelium permeates the stick and is able to survive for longer than the mycelium on the agar surface. It provides a protective barrier from outside elements and disturbance. Its like a fail safe for your culture in case the agar dries up you can pull the colonized stick out many years later and revive the mycelium.
@@SporenSprout Very cool, Thanks for the response.
@@SporenSproutfascinating. Where did you learn about mycology? Do you recommend any books?
Hey brotha, so I made slants today for my gen1 LM, but I forgot the damn popsicle sticks!!! How viable are these gonna be for long term storage? They are the same tubes you got.
They should still be viable after one year. I would transfer it to another fresh slant next year.
@@SporenSprout thank you sir! I just watched your video on the top 4 medicinal mushrooms and I think I want to try growing them. I’m gonna try my hand at a double extraction tincture w some of the lions mane I grew this weekend. Well, at least get it started.
@@sugaslate14 Thanks for watching that video! 🙏 Congratulations on your successful lions mane grow! That’s awesome 👏 🍄. Dual extracts are the best way to go, good luck!
@@SporenSprout hey brotha, how can I store my lions mane after harvesting. At least for a few days without them turning brown or getting fucked up? I’ve got this second flush wrapped loosely in parchment paper, and in a bowl on the counter
@@SporenSprout hey man so it’s been I think 6 days and my agar slants aren’t growing any mycelium. I took tissue samples and have em in the proper environment. Did I do something wrong?