How to Make No-Pour Agar Plates for Growing Mushrooms
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- čas přidán 29. 06. 2024
- This method of making re-usable agar plates is super easy and reliable. You don't need a laminar flow hood or still air box to pour the agar plates because they are poured before sterilization. The twist top eliminates the need for parafilm around the edges. I have not noticed any problems from lack of air exchange for the short term. If you plan on using them for longer term storage i would loosen the lid and wrap in micropore tape or parafilm.
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Ive been using this technique for a couple months. I ended up saving a good amount of money but it was a bit challenging for a novice at sterile technique. The drawbacks are condensation and difficulty working with one hand.
For condensation: When my plates leave the cooker they are stored upside down so any residual water falls to the lid. When I use a plate I unscew it upside down and right it. Then I let the water drip off the lid before I screw it on.
One handed: When I use traditional petri dishes its so much easier because one hand can hold a scalpel and one can manipulate the plate. Unscrewing these with one hand is possible but it takes a little dexterity.
Keep in mind that these are smaller than petri dishes so I typically use the whole plate to innoculate grain.
Buy more than you think you’ll need and buy your agar mix in bulk to actually save money.
Nice, I first saw these containers being used by Mossy Creek. They provided a bulk warehouse link that is way too costly for me. Thanks for sharing this Amazon link. Will try!
Omg, I'm obsessed with your mushroomy heat mat!!
Great idea I will try it 🙌
Thank you for the video
Thanks for watching! 🍄
(1st Question) How long can you wait between pouring these and pressure cooking them (2nd Question)How long can you store them after pressure cooking before you use them in a SAB or in front of a Flow Hood. And would you recommend learning to do Agar or Liquid Culture Method first? (I know people often use Agar to LC, or Agar to Grain). I'm Currently Working on growing Yellow Oyster Mushrooms with your instructions!
Thats great
Thanks for watching! 🍄
Great videos and products. 🍄🟫
Thank you! 🙏 🍄
Lolz amazon needs to give you a discount, since you shared those twist tops everyone bought them all up. Its been 3 weeks and they stay sold out .😂.
Dang that’s crazy 😂
I love this, its a great way to cut down on the waste we produce. What are your thoughts on how thick that your agar is? Is that just using what you make or do you actually like it that thick? I usually pour my plates about 2mm and ketchup cups are thicker which I like for keeping samples longer. Keep up the great work.
Yeah I was just trying to use most of what was made but I do like thicker agar so it lasts longer and doesn’t dry up.
Could you do this with glass petries and parafilm? Just load them up before sterilization?
thanks for this video! typically use ketchup cups since they're easily accessible for me and less hassle than the petridishes but these look fantastic. I'll definitely be giving them a try, are they able to be sanitized and reused?
Yes they are awesome! They can be cleaned and reused.
like your agar content! Are you from SagNasty? i grew up there
Thanks for watching! Yes I am 😂 that’s crazy!
Hi,
Might be a stupid question but what are the main reasons for using plates, liquid mycelium, and grain?
How do you use them, and why?
Many thanks
Watch this video:
czcams.com/video/EARNzNHAzf0/video.html
It will answer all of your questions.
Don't they loss their shape a little bit when sterilizing because of the temperature and pressure? I know it's PP, but I use another brand of PP plates for agar, and they get deformed a little bit each time I sterilize them.
No these containers don’t warp or become deformed after pressure cooking. I have re used them multiple times and haven’t noticed any difference.
Hey men ! I already tried with these plates in a presión cooker, however, when i opened it after cooled down all the plates were deformed from the pressure and the agar was still liquid. Any idea why?
Sounds like you didn’t leave the lids loose while pressure cooking
Wish I could get containers like this in Australia, but for some reason this seems to be a US only product for now
ScienceEquip online store sells polypropylene petri dishes, it's just they are not twist top. I have a bunch at home, so will test it out and see at some point using the above method, except sealing once sterilised. Also might need a weight on top so there is no boil over in the pressure cooker.
@@mlowes23 the twist top is the major selling point for me - I'd be happy to never have to cut and wrap parafilm
@@ahosie I use glad wrap, but agree and would also eliminate more plastic waste with the twist top
on ebay there are aussie made ones (can see Product of Australia in the photo) but i'm waiting shipping so grain of salt
"250ml 500ml Plastic Clear PP Round Jar 90mm Diameter Screw Lid Food Storage Box"
different dimensions but look usable and are PP dishwasher/microwave stuff, seems legit
Try Temu!! They have all sorts of containers.
This seems great, but I just can't get them to work out. Almost every one has water sloshing around on top of the agar, even if I let the plates cool considerably before pressure cooking. Any advice?
After pressure cooking and solidifying, place them in a hot room. That will solve the problem, agar will absorb the moisture over a few days in hot room
@@SporenSprout I'll try that out and see how it goes! Thanks!
Will they last 45+ mins at 15psi, say if I wanted to sterilize some grains at the same time?
Yes definitely
@@SporenSprout awesome they are in there now, thanks for bringing this tek to my attention.
Is it enough to use only agar agar???
Agar agar and water will make “water agar” which is good for cleaning contaminated cultures but produce weak mycelium with no nutrients. The mycelium still will grow but faintly
How u iniculate the container
Just twist off the top and put a drop from a syringe in the middle and put the top back on.
I put an injection port on mine and a filter just like I do on my grain jars.
Ok so I'm confused. It's "no pour" but you are pouring it into the dishes. So how is it "no pour"?
No pour refers to not pouring post sterilization. He pours pre sterilization thus reducing the chance for contamination
@@GrowYourOwnFun still doesnt make sense to call them "no pours"
How else would he get them in to the plates? Lol
I earnestly agree, the term should be changed as it makes zero sense. The method should be called "pre-PC pour".
@@MissBlackMetal I think pre-pour would suffice, but I agree completely with you
hey i tried the discord link, it links to your discord but it says unable to accept invite
Thanks for letting me know, here is an invite link: discord.gg/atfa4BZpBM
I’m not sure why that happens sometimes.