The PhotoFridge - Cubane Ep 13
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- čas přidán 17. 12. 2022
- I'm a simple man trying to drive a simple photochemical ring closure reaction. What could be more simple?
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Music: from the Aphex Twin soundcloud dump. Track name: Pretend Analog Extmix 2b
References for the whole cubane synthesis: 1997 Lab scale paper: www.researchgate.net/publicat...
2013 Pilot Scale: pubs.acs.org/doi/abs/10.1021/...
"Cubane Derivatives for Propellant Applications" 1989
apps.dtic.mil/sti/pdfs/ADA210... - Věda a technologie
Ok important question though: what are we calling the thing that lives in my walls?
Severe lack of medication
A tasty snack.
Johnathan
Quentin Tarrantino
Martin
"sick of this contamination shit" he says as he does organic chem in his garage
crack shack*
with a post apocalyptic looking mobile fridge
Never seen him run a flash chromatography. I understand that it's not very practical nor economical to do while funding the solvents yourself nor remotely safe without a proper fume hood, but you can't expect your product to not be a mess of different compounds after several steps. And it compounds, because without being certain of decent purity after each step you have no way to do the stoichiometry accurately either.
don't forget that nilered once cooked a spider with his boiling mercury
@@takeawaykitty. No no, he's made it very clear there's no drugs. It's a rat shed that also occasionally has snakes.
So I'm a PhD student that has had the misfortune of having to work with compounds with the same functionalities as your product. Trying to analyse them with TLC is a real pain. There is nothing to oxidise, so KMnO4 does nothing. They don't absorb UV-light. Iodine only sometimes work. I saw some other comments suggesting some more exotic stains, which I haven't tried, bit I think there is one more accesible one that you should try first: vanillin. Vanillin is an aldehyde, that, if activated by a strong acid, is a very strong electrophile that reacts with essentially anything nucleophillic. The products are often coloured, so they are very easy to see on TLC. Ketones can under acidic conditions tautomerise to form nucleophillic enols, and I've had some success with it for this type of compounds. It also smells very nice.
To make the stain, just dissolve vanillin in 1% H2SO4 in ethanol. You will need to heat the plate quite a bit to see anything, but if it works, it should be pretty obvious that it did.
Good video as always, BTW, I think you are pretty close to figuring this out now.
Comments so he sees this more bigger
And anisealdehyde
Things are gonna taste yellow
my question is why not spectrum machine? he is still a phd, right?
Yea Vannilin is a good one to try. Neat bonus: it smells really nice!
NileRed: Rents an entire island to boil acetone
Ex&F: Spends years attempting to synthesise an incredibly complex molecule using hot glue and a tin can
The lowest effort
@@mamupelu565low effort? I would say this is high effort to do it in the most complicated way possible
We need to get a "Have you seen this molecule?" message on the back of every milk carton
And then a shirt of such a milk carton image for merch
@@ripvanwando I'd buy that.
That was exactly what I was thinking too 😆
@@ripvanwando seriously, that is *so merch*
@@kumoyuki i'd wear that
Wake up babe new cubane video
I haven't been this excited for a CZcams video in a minute.
Yes wake up from your 7 month coma
LITERALLY
@@stutterpunk9573 punk love!
forsen1 I C BAJS
The project has been going for over 2 years already? Damn life moves fast.
Yeah man too fast
@@ExtractionsAndIre Life moves fast, chemistry doesn't. I live my life a 0.25 mol at a time.
No wonder it feels like this series has been dragging on for so long, these last few steps have put grey hairs on my chin.
I'm glad to see the vids back. I have the playlist saved and every so often I saw it on the list and thought, "hope the cubane guy is doing OK"
2020 didn't happen so we kinda lost a year
I feel like having a good pair of tweezers might improve this man's life significantly.
Yeah they'd make it much easier to catch that funnel web spider hiding in the walls
I was thinking that a pair of kitchen tongs would make it easier to put the cuvette in the photoreactor.
Or even just tongs
@@ryanc473 silicone ones might be useful, if you can get small ones, for friction
The guy has hemostats from an old family vet practice. He just doesn’t use them.
The contamination is probably just the spider pooping in your DCM as revenge.
Albeit a small thing I think what happens at 6:59 is a good descriptor of why we adore your channel. "i have this cuvette full of a solvent that can apparently dissolve my table. let's test if the lid leaks by trying above said table". Just the right amount of carelesness that paints you as a real human who isn't forced by some higher up lab person to do stuff as the protocol says. Thanks for your work, E&F!
im just here for the spiders mate
and I had the metal lid RIGHT THERE but no I had to test it over the paint - why did i do that
@@ExtractionsAndIre it's funny because it literally is paint stripper
@@Mister_Brown It's also delightfully carcinogenic, and absolutely stellar for smoothing and solvent welding PLA.
@@AlexanderTzalumen It’s still funny to me that I can buy cans of the stuff down at Bunnings lol. Useful as hell though but Christ distilling it S U C K S
Ep 13 He is in my walls.... Ep14 turns out she was in my walls... now they are everywhere
Ep15 - Trying to kill the things in my walls with white phosphorus
@@RussellTeapot Ep16 - I’m going to The Hague! (not for a conference)
Ep 15 Drums in the deep - they are coming.
hey, lab assistants!
Lucky he a found a box of deadly, questionable pesticides....
Speaking as an electronics engineering tech, I have a weird amount of pride watching someone learn and build a simple circuit. This definitely made up for last episode, lmao.
haha yes redemption !!
@@ExtractionsAndIre I mean that resistor spider made me weep, but at least it didn't make cry out like yeeting a whole amp through an LED did
@@ExtractionsAndIre use a quartz vessel, glass absorbs UV
@@fss1704 He is.
@@Tunkkis i saw that later, forgot to delete the comment.
Came for the chemistry, stayed for the curated Aphex Twin soundcloud dump playlist
this
@@adfaklsdjf he always does that for this side channel, its great
i love the victoria bitter in the background while synthesizing an explosive chemical. thats my aesthetic right there
Bikie chic
The real question being what's actually in that bottle.....
I started watching this because of organic chem in 1st semester, I am now about to start writing my bachelor's. How time flies
I started watching this channel at the beginning of my bachelor too, hoping that I would some day understand all the chemistry he's doing. I'm now in the process of writing my master's and I still have no fucking clue what's going on in this man's lab
yeah and im just starting my bachelors in chem and i've been following this for a few years now. Every now and then I get excited because I understood one thing or remembered a term :D
Hi I’m a Bachelor’s student in chemistry and over the summer I worked on some photochemistry stuff and last year got into a lab doing some cubane-like synthesis. As commented before, resulting to TLC is sometimes very difficult with these syntheses and if you can, I’d honestly recommend seeing if you could have your friend with access to the NMR do some characterization. Also! Please wrap your reagents and products in aluminum foil if they’re photoreactive. Most photochemical reactions are reversible and if you expose them to a continuous spectrum like white light, you’re just asking to undo your progress. Hope this helps.
Mate, if he hasn't wrapped in foil after two years. He ain't gonna wrap them. He's a PHD too.
he did mention in the video that he stores them in a place that doesn't receive light.
@@TheBoshy he's not a chemistry PHD tho, he did his in physics. He's a good chemist, but no where near PHD level.
Pretty sure NMR is out of question if he wants to keep this “out of my garage” theme going, hence why he’s sticking to TLC, unless he pulls out a rusty NMR machine from the university kerb. That said, would GC-MS be feasible? Pretty sure there are companies you can submit samples to for analysis (and to keep with the theme)?
@@ghindegaming2222 I have a feeling these molecules are too heavy for GC, but maybe normal phase LC could work. That's more expensive and specialized though.
I love the "found footage from a meth lab" energy of this series
Using hot glue on heat sinks is probably one of the biggest DIY power moves, kudos. Also the new lab coat looks nice
it's an indicating adhesive, if it gets too hot it will melt the glue
@@Mister_Brown Thermal fuse, exactly!
I feel like we're somehow watching the first channel able to rebroadcast after The Incident, streaming back in time from 2054.
So that’s what happened in the few days that turned into months in there.
the cataclysm has destroyed the concept of time during portal storms
DO NOT TALK ABOUT THE EVENT. They don't like it when you talk about the event!
@@sir_vixRemain indoors!
Hello, good evening and remain indoors! czcams.com/video/wnd1jKcfBRE/video.html
I love the juxtaposition of buying expensive quartz containers for your food-can-and-hot-glue-and-archeological-relic-fridge photoreactor.
Not pHd level anything, but just a heads up on using the ir thermometer: the laser is deceptive because they actually read in a cone shape (the angle of which will be on the label), and in addition aluminium is relatively reflective. So when you're measuring the heatsink temperature with the gun, you're likely actually measuring the heatsink and maybe the a 50c coin area around it on the Milo tin (and that temperature will also be skewed to the temperature of the tabletop by reflection).
Yeah I feel that. But it mostly is there to try and prevent me from grabbing things to see if they are super hot or are room temp
@@ExtractionsAndIre didn't look like it lmao
@@crackedemerald4930 Tbf, he did write "try".
Yeah, this.. I'm saddened by the major caveats that come with these "laser" thermometers :( you can compensate for the cone/spread by moving it closer, but the emissivity issue foils me. Would marking it with a sharpie help? Piece of masking tape?
@@adfaklsdjf black electrical tape works pretty well
> hmmm how can I keep this cold?
> pulls out WW2 era fridge that is clearly home to 1000 redbacks
It’s certainly a solution
That compound is a ketone, so it should give a spot with Brady's Reagent. Also' if your solvent is giving a spot then you should distil it. Maybe the manufacturer has added a stabilizer such as hydroquinone.
Ok great I’ll check it out!
And the solvent is fine by itself, but not after it has spent any time in the cuvette with the lid on
@@ExtractionsAndIre maybe try washing the cubette with some other solvents instead because they could potentially dissolve it better than the dcm.
Also try putting the stopper in some normal glassware and filling it with dcm before running the tlc on that dcm to determine if it really is the stopper.
I like this idea- probably the most low tech and solution to elegant solution to the visualization problem
@@Stalutes64 Perhaps it's not the stopper, but residue that's leached out of the teflon tape. If that's the case, removing the residue might be a possibility.
@@PSUQDPICHQIEIWC in fairness if the stain isn’t working maybe the quartz glassware isn’t actually necessary and it just wasn’t being detected when using borosilicate ?
Yeah bro, UV chem in the fridge. What a day to be alive
Watched the whole thing and loved it
speed run
24 minute video in less than a minute, time traveler confirmed
@@thom534 Time traveler or early access Patreon?
@@sammiller6631 way to make me expose myself as not being a patron
Love when the fridge looks like it still uses banned refrigerant lol
Netflix needs to upload this series at some point on their site.
As someone who’s made cubane, I find that recrystallizing the intermediate right before the UV cycloaddition is incredibly helpful. You can use UVA lamps for it (I think the key wavelength region for this reaction is 300-350 nm). I run this step in methanol with a slight amount of HCl (1 mL for 300 mL MeOH). You should start with a colorless solution and end up with a yellow solution that concentrates to an orange sludge if it works. Just need to blast it with photons. Tsanaktsidis has a great prep from 1997 in the Australian Journal of Chemistry
I have so much confidence that I will bet someone money that Tom successfully synthesizes cubane before 2056. Any takers?
I think by 2043 it will be available in his nearest hardware store :)
Though it will soon get banned for being the nearest precursor to a very potent euphoric designer drugs family of cubane-propan-amines ;)
Did you try Cerium Molybdate (Hanessian's Stain)? Works really well on things KMno4/ I2 wont spot. Heres a recipe: To 235 mL of distilled water was added 12 g of ammonium molybdate, 0.5
g of ceric ammonium molybdate, and 15 mL of concentrated sulfuric acid.
From my mom, who is ABD in analytic photo-electro-chemistry, after watching:
"The quartz cuvette and buying cheap low quality materials... This is my phd omfg lol I can relate sooooo much!!!
Also, a uv-vis absorption detector had to be fairly cheap nowadays... HAS to be better than running endless TLC plates and trying to dye them???"
I crave the cubes! I'm so happy this series is continuing. Also that game cube into will never not have me in stitches.
Möthër, Ï crävë C Ü B Ë
Its another Cubane episode, hype time boys!
There is an unmistakable correlation between the end of the academic year, and an increase in E&F videos...
Gotta love the old Engel that looks like it's been at the bottom of the ocean for 30 years that still works perfectly
11:00 bro was definitely not a funnelweb, just a brown/grey house spider (Badumna longinqua or similar). They just kinda build their webs like that
Funnelwebs live under rocks and logs and shit exclusively on the ground, also they hate people and are only found indoors when its a male trying to find a mate
When you run a chemistry channel you're gonna get some bio nerds too 🤓 🤓
This legit just made my day. Thank you, you chad genius.
Holy shit, I hadn't been following the synth for a couple episodes, so I was catching up last night. Wake up to the first upload in 7 months and it's the next episode. Something, something, energy manifesting Aussie organic chemistry man slowly progressing towards very tiny cubes.
I was literally just re-watching threw the cubane series last night. I love watching your videos keep up then great work.
Your soldering iron looks like it could burst into flames any second
Don't worry, the crust acts as a fire retardant
Looks pretty normal actually. Actually, soldering looks pretty good, at least at a glance. Clean(ish) tinned iron, good solder coverage, using what's needed not globby. Not bad for a filthy chemist!
2 year old reagents was as much of a reality check for me as for you I think, I've been following the whole way. Kudos for having the determination to grind through this, and I super duper hope you get your miracle reference sample.
You couldn’t be more different from NileRed, yet you’re equally as fun to watch 😂
You couldn’t be more different from NileRed, E&I actually posts videos occasionally.
No. He's way better than NileRed
Nigel is a craftsman, Tom is an artist,
Old NileRed vids were a lot like this chemstry wise, he just went to normie chem after chem channels started being shut down by youtube (Rip ChemPlayer)
@@Kwazzaaap is Nurdrage still around?
i understad less than 80-90 % of the thing that happens/ is talked about in your videos, and yet i love watching them. GREAT work, keep it up
@extractions&Ire I’ll make you a Quartz test tube…..shoot; I’ll make a whole set for you. What dimensions do you want?
This is probably not the comment you're looking for, but as a multi-disciplinary nerd I can say that you have the *best* reality TV show ever. I am so hooked with all these cliff-hangers! :)
If you ever feel the need to upload to the other channel to blow off some steam (as it were), I'll be there for that too )
1:55 "It wasnt very hard, I just didnt think about it properly"
Thats kind of what this channel is all about isnt it
This is a Christmas miracle. A joy to see.
You legend.
Light the Cubane cigars boys, the chemical kangeroo is back!
When I saw the latch on the fridge, my immediate thought was "must be to keep kangaroos out". Not sure how I feel about myself right now.
Love your content... I get more excited when I see a new E&I video than any other channel. This has been your best series. Don't give up
Love the Channel have been watching this since the start! Trying to figure out how to show this to my Chem class (High School level) to show them real Lab Chem and stick-to-itness as well as the NaCl (y) language that we all hear in the LAB!!!! Again great stuff!
Suggestion: invite (Kidnap) Nilered and have him be your lab assistant. That way, if the synthesis continues to bedevil you, you can blame incompetent lab help for dodgy results.
I don't fully understand why but I find your videos really soothing and relaxing. Thank you, you make me proud to be Australian 😂
you are my hands down favorite content creator!!! Thank you!!!!
Ive been following this series for so long lmfao, thanks for not giving up on this pipe dream xP
Always love to see when an upload of yours reaches us Tom❤
You actually were part of the reason i decided to go and go do a bachelors study myself (3/4 year RN) and its been a pretty okay few years so far (not looking forward to the thesis tho😂)
Try a vanillin stain, 1M vanillin + 1% conc. H2SO4 in ethanol... they show up "most things" with some pretty colours. Baseline spot implies the product is being oxidised as whatever you're forming is polar as hell - dissolved O2 is usually a killer of photochemical reactions, so you could always try sonicating / sparging with argon before it goes in the reactor. Bromocresol green is good for carboxyllic acids, so it could definitely show you what you're not supposed to have :)
You’re one of the creators I get really excited when I see a new video comes out!
I get so happy whenever a new cubane episode drops
Watching you shake your fist in frustration not at the experiment itself but the failing methods of determining if the experiment is even working is either validating or relatable or both haha
I got a job in molecular/cell biology as a stoic exercise and I say just quit being a coward and bet it all on an un-stainable clear colorless solution. Pretty much what I do all day for a living.
Haha yeah I like that approach. Just go onto the next step after putting it in front of the lamps for a few hours, no checking, just believing the product is there. Manifesting it
Absolutely love your work and your videos. I wish I understood chemistry at the level you do but it’s epic to learn and watch. Have a great Christmas
ngl you make very enjoyable videos and I love to whatch them, thanks for uploading once a year or so
I really love your videos. You have so much knowledge about chemistry, and present it in a way that makes it a lot more realistic than anything else I've seen. A quick note about those resistors: I'm not sure how the LED clusters are wired but I'm pretty sure you're not driving them as hard as they should be. 19V - Forward voltage = 16V. 16V / 1k = 16mA. So you could drive them twice as hard if you wanted to. Not sure if this helps, but if it were me I'd try some 470 Ohm resistors instead of the 1K resistors you've got.
That was my impression too. Intensity seemed rather low.
A CHALLENGER APPROACHES. Lumiray has started a cubane synthesis video too. Let's see who can get it done first!
Love these videos man, may your products be always of high purity
Absolutely love the narration. Brilliant!
great tune choice as usual, hopefully warp wont complain as this one was also included on the re-release of orphaned deejay selek
Ooh yeah I hope not! Have got into issues with a Soundcloud song before that was similar to a release or was on a release I think
Hot glue on the hot sink oh Tom you make me holler :’D Also this is my favorite series but for your sake I hope it ends within the year mate
I always get so excited to see your videos pop up, you made my day sir
I just binged every episode of the cubane ExPeRiEnCe. What a journey. I'm so damn invested in all of it
I feel like your previous episodes were just yesterday 😭 Its been 2 years?!?!? Im getting old... Atleast every upload of this project makes me feel young again.
Would the next step be as hard to confirm as this one? Perhaps it would make sense to just try it as you suspect that you may be making the right product but not detecting it.
I have no helpful advice, just wanted to say I love your videos and can't believe I've been following the cubane series for two years now!
Chemistry postdoc here. Potassium permanganate is a good stain for your starting material but probably not your product (as you've already discovered). Vanillin could work also. 2,4-dinitrophenylhydrazine should be a good stain for both the SM and product, but I've never used this before. Hanessian's Stain could be good because it should give different colours, like the vanillin stain.
The easiest way to get a good idea of product formation would be FTIR (infrared spectroscopy) because you should see 2 distinct carbonyl peaks, run this on your SM and also some crude product - you've used help from friends at the uni before so could be useful again? NMR would also be useful (but hard to interpret), and LCMS would be great because you'd just be looking for a single mass of your product. Not sure if you have access to this equipment though.
Lastly, PLEASE use PTFE tubing as a reactor and not some cuvette or other vessel (glass/quartz whatever). You can get a spool of really small diameter PTFE (or PFA may work too) tubing for cheap, like if you get 1/32" inner diameter tubing and use this as a reaction vessel you get insanely good UV penetration into the reaction medium. Inject your reaction mixture into some tubing, cap both ends with PTFE fittings, place it in your fridge reactor and let it go. Look up 'continuous flow photochemistry' on Google Scholar for more information.
Really love the vids and want this to succeed, good luck!!
I've honestly never though about directly using PTFE as a reaction vessel... but if it gets good UV penetration, it's a great idea!!
Also I think Vanillin stain is a great suggestion
You'll get there Tom, believe that truly your level of awesomeness cannot be surpassed
I’m working on cubane myself but probably won’t be at this step until you’ve figured it out. Have honestly considered using carbon tet. Wanted to try and follow the paper as close as possible and then tinker around with the UV step and different solvents to see if there’s a good way to go about it. Looking to get all the way to cubane of course but what I’m really interested in is the material in the last step because you can do some funky stuff with that to make different cubes.
Best of luck with it mate! Yeah if we could work out some good conditions and how to easily detect the product with TLC, I think the whole cubane synthesis could become quite repeatable and accessible for the home lab
@@ExtractionsAndIre based on the small amount of products you're getting for each step, will you be able to properly test the final octonitro compound? If not I've heard that cubane is available from some pyrotechnic supply sites.
@@ExtractionsAndIre agreed! Will share any findings. Definitely plan to mess around with that step a bit! The answer is out there somewhere lol
have you considered setting it up the other way round - i.e. using a UVC bulb, shielded by a quartz tube glued onto a ground glass joint, and inserted into a borosilicate flask?
I've given some serious thought about how to carry out the Pettit synthesis of cubane at home, and this is the most foolproof design for a photoreactor that I came up with
I think this would work but the heat sinks would have to go somewhere. As it is the bulb would heat up and evaporate the solvent
@@forwhomthetacobelltolls9789 you could immerse the borosilicate flask in an ice bath
also, the UVC tubes I've come across have been LED/mercury vapour hybrids, with a power rating of 20 watts or less, so they shouldn't produce huge amounts of heat
i thought this series was lost to time! so happy! lets watch!
I'm more excited for this video than Christmas morning!
If you put some silica with the iodine in ur chamber and then shake it lightly to move the silica over the plate, it will stain 100x faster! Just the vapors sometimes do a horrifically slow job ;(
ay pro trip, thanks!
Personally, I would work to clean up your lab area first. Seriously fix things up. Fill, seal, and paint the walls. Seal the room from the outside. Maybe put an air purifier in there. Might just be dust that's getting into your stuff and making a mystery dot on the TLC's.
Somehow I found this channel about 5 years ago and it's still gripping
YES what a great day! A new Extractions & Ire video! Thank you for posting
iirc, some people in the comments of soome of the other videos suggested that you could get quartz tubing from UV sanitizer replacement tubing as the UV sanitizers use quartz tubing in them. Did you ever check that out as a possible avenue?
Possibly! But how do I seal it well in a way that keeps the volatile solvent in?
No of course not
@@ExtractionsAndIresince you only need the part of the tube holding the solvent to be UV transparent, you could always take a hacksaw to some (clean) broken test tubes of roughly the same diameter and melt the rim of the cut line on the test tube to the rim of the cut line of the tube make a weird ad hoc chimera ampule where the bottom is the quartz glass and the top is normal glass. If both sides of the quartz tube are open, you can melt the bottom of a broken beaker to one of the sides before putting the solvent in to act as a base.
Sounds perfectly in line with the rest of the stuff you make ngl.
@@ExtractionsAndIre I’ll make you some Quartz test tubes if you want.
@@meme__supreme3373 is it possible to adhere dissimilar materials like that?
Maybe reach out to thylabs and see if he's got any advice/the molecule you're looking for. He's doing a cubane synthesis right now, though I think it's a different route than you're taking.
yes I talk to him and maybe together we can get past this bloody step!
@@ExtractionsAndIre I'm sure you'll get it figured out bud. Good luck! Thanks for the reply
9:18 *slaps top of fridge* You can fit so many Cubanes in this baby!
I gleefully giggled giddily when I saw this pop up in my feed. Welcome back Cubane.
Cool
Do UV lasers come at the wavelength you need? Its been so long i don’t remember the range you need
300-350nm. Not really an easy laser wavelength unfortunately!
@@ExtractionsAndIre what about a laser diode from a dvd/cd burner? My dad and I used to rip open anything with a disk drive for the diodes, half of them produced UV, not too sure of the average range I suppose.
@@azraellie_ around 650-675 on dvd and blu ray.
@@ExtractionsAndIre what about a bandpass filter?
You just gifted me a good night of sleep. Thank you.
I do not know what the hell you are doing, or why. However, I am thoroughly entertained! Glad to see another video from you!
I uh dont know why I'm subscribed. Who are you again?
This was worth being @all’d
This is the first time anyone has every said this in the history of discord
thank you for coming back and sharing chemistry science
Great job finding the impurities and contaminations! Those things drive me crazy. I'll never forget being stuck with the one breadboard in electronics lab with the broken row.
The row that only makes a connection 90% of the time
for the uv side of things have you considered a curing machine for resin 3d printing something like the Elgoo Mercury
Love your videos. Best wishes on an ambitious project.
If you can buy curcumin powder from a health-food store or the supplement isle in a grocery store, you can make a saturated solution of it in absolute ethanol containing 1-2% sulfuric acid. You should be able to then dip your TLC plates in it or spray it on a warmed (45 C) TLC plate (pre-heating it helps “fix” the spots so they don’t run like wet paint). Re-warming the plate carefully should reveal any ketones as deeply colored spots on a bright yellow background. Amino acids should turn bluish. Ketones should become deep red or deep orange.
Curcumin is a beta-diketone that has a very nucleophilic enol, and a very electrophilic oxonium ion (a proton gets coordinated between the keto and enol oxygens, making a very electrophilic system). If water is present during heating with acid, it hydrolyzes to even more reactive substances (a substituted acetoacetone and a molecule of vanillin), hence you don’t need to hunt down vanillin. It’s like vanillin (a solid vanillin precursor) because with water, acid and heat it should generate vanillin right on the TLC plate. (Caveat: I've tried it in a limited number of situations and it worked, but I've never seen a literature reference, nor a recipe for it online).
This is the most suspenseful series ever.
Nice to see ya buddy, ol pal, commenting before watching. My day got 15% more brighter cause of this.