Determination of Unsaponifiable Matter of an Oil or Fat Sample (Part-1)_AOAC 933.08 & IS 548-1

The term "unsaponifiable matter" refers to those substances present in oils or fats that are not saponified by alkali hydroxides and are extractable into ether. It is a very important testing parameter for oil or fat sample. This video represents Part-1 for the determination of unsaponifiable matter of an oil or fat sample. The video link of Part-2 for the determination of unsaponifiable matter is given below. __________________________________________________________________________________________
**** Video Links ****
Unsaponifiable Matter (Part-2): • Determination of Unsap...
Saponification Value: • Determination of Sapon...
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[@annag3373]

Do you know if carrot juices require saponification, too? Do you extract beta-caroten from fruit juices only by centrifugation without saponification?

[@Titi-je3ff]

Thanks a lot

[@MicroChemsExperiments]

Kasthuri kk, its pleasure

[@roshan.Lab-91ok]

Thanx for upload this vedio

[@MicroChemsExperiments]

Thanks

[@purnimapatel3483]

I used petroleum ether for extraction. I got separate layer bt by washing ethanol and distilled water It did not separate what can I do.

[@MicroChemsExperiments]

Ethanol is fully miscible with water. So you will not found separate layer between ethanol and water. During this test, ethanol will be discarded along with the water. Just collect the hexane layer after washing.

[@beatrizcampelodasilva6536]

after the saponification step, my solution turn solid, forming like a gel, when cooled. what may had happen?

[@MicroChemsExperiments]

If you face to form gel in the separation funnel then it is called emulsion. To remove emulsion, add ethanol and shake again

[@RadheySingh-bg4ed]

Very nice and work helpfull

[@MicroChemsExperiments]

Thanks a lot

[@asifashraf6235]

Sir i shall be thankful to u kindly send the testing method of fragrace purity

[@MicroChemsExperiments]

Sorry, we don't provide any document

[@narendarpratapyadav9908]

Hello
Sir give me some information of NPN check in maize gluten please
Thanku..

[@MicroChemsExperiments]

We will make video for NPN later. Stay with us

[@alaminlemon8544]

Please make video 0.1 NAOH solution with Standardization

[@MicroChemsExperiments]

Alamin Lemon, we used commercial standard 0.1N NOH solution (ready made) in this test, that's why we didn't standardize our solution. In future, we will make video of a procedure for standardize different solutions including sodium hydroxide.

[@amrishsinghparihaar6529]

Thanks

[@MicroChemsExperiments]

You are welcome

[@alibhutta2799]

while performing experiment everytime emulsion is formed in which step am doing mistake pls Guide

[@MicroChemsExperiments]

Please add extra ethanol to the emulsion and shake. The emulsion will be cleared out

[@alibhutta2799]

After 14th wash no clear layer formed at top what will be the reason ?

[@MicroChemsExperiments]

Loss of hexan with emulsion during washing the extract.
Wash it more carefully

[@goda2002]

thanks

[@MicroChemsExperiments]

You're welcome!

[@alibhutta2799]

Pls Guide can I use 0.5M ethanolic KOh instead of 0.5M Aqueous KOh

[@MicroChemsExperiments]

No. You can't

[@alibhutta2799]

@@MicroChemsExperiments JazakAllah

[@user-gw8ce9je3k]

what is the purity of the KOH pelltet?

[@MicroChemsExperiments]

99%

[@felipebrondani8167]

Hi. Can I do with less than 2.5g of oil?

[@MicroChemsExperiments]

Yes. You can do

[@Wardi_lovers]

Sir please provide us "how to analys sugar percentage in crude"
I hope you upload the analys video of crude sugar percentage soon.
Thank you 🙏🏻

[@MicroChemsExperiments]

Noted. We will upload the test as early as possible. Thanks for being with us.

[@MicroChemsExperiments]

Note. We will work on it soon. Stay with us.

[@pranaysarkar1611]

Lots of love

[@MicroChemsExperiments]

Pranay Sarkar, Thank you so much.

[@hafizishfaq8319]

Thanks. Very interesting method

[@MicroChemsExperiments]

@@hafizishfaq8319 It's our pleasure. Stay with us.

[@purnimapatel3483]

Please send me reply as soon as possible, because I m doing this practical now.

[@MicroChemsExperiments]

Already replied

[@manoranjansahoo8586]

Engine oil test

[@MicroChemsExperiments]

Noted

[@harshitakaushik9058]

According to IS 548, nowhere they have used hexane. Why is this video using hexane?

[@MicroChemsExperiments]

Petroleum ether and hexane are very similar in chemical properties. You can use both

[@harshitakaushik9058]

Great, thanks. I'll perform this experiment today and comment here if I have any doubts

[@MicroChemsExperiments]

@@harshitakaushik9058 go for it

[@samiyainspiration]

Thanks a lot

[@MicroChemsExperiments]

Most welcome